MicroRNA-98 and let-7 regulate expression of suppressor of cytokine signaling 4 in biliary epithelial cells in response to Cryptosporidium parvum infection

Guoku Hu, Rui Zhou, Jun Liu, Ai Y. Gong, Xian-Ming Chen

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Expression of the cytokine-inducible Src homology 2 (CIS) protein and suppressors of cytokine signaling (SOCS) proteins represents an important element of host cell reactions in response to infection. We have demonstrated previously that Cryptosporidium parvum infection down-regulates microRNA-98 (miR-98) and let-7 to induce CIS expression in biliary epithelial cells. We report here that down-regulation of miR-98 and let-7 also coordinates epithelial expression of SOCS4 after C. parvum infection. Targeting of the SOCS4 3′ untranslated region by miR-98 or let-7 resulted in translational repression. Functional manipulation of miR98 caused reciprocal alterations in SOCS4 protein expression. Transfection of miR-98 precursor abolished C. parvwm-stimulated SOCS4 up-regulation. Moreover, expression of SOCS4 in epithelial cells showed an inhibitory effect on phosphorylation of signal transducers and activators of transcription proteins induced by C. parvum. These data suggest that miRNAs play an important role in the coordinated regulation of CIS and SOCS expression in epithelial cells in response to C. parvum infection.

Original languageEnglish
Pages (from-to)125-135
Number of pages11
JournalJournal of Infectious Diseases
Volume202
Issue number1
DOIs
StatePublished - Jul 1 2010

Fingerprint

Cryptosporidium parvum
MicroRNAs
Epithelial Cells
Cytokines
Infection
Down-Regulation
Suppressor of Cytokine Signaling Proteins
STAT Transcription Factors
3' Untranslated Regions
Protein C
Transfection
Proteins
Up-Regulation
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Immunology and Allergy
  • Infectious Diseases

Cite this

MicroRNA-98 and let-7 regulate expression of suppressor of cytokine signaling 4 in biliary epithelial cells in response to Cryptosporidium parvum infection. / Hu, Guoku; Zhou, Rui; Liu, Jun; Gong, Ai Y.; Chen, Xian-Ming.

In: Journal of Infectious Diseases, Vol. 202, No. 1, 01.07.2010, p. 125-135.

Research output: Contribution to journalArticle

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abstract = "Expression of the cytokine-inducible Src homology 2 (CIS) protein and suppressors of cytokine signaling (SOCS) proteins represents an important element of host cell reactions in response to infection. We have demonstrated previously that Cryptosporidium parvum infection down-regulates microRNA-98 (miR-98) and let-7 to induce CIS expression in biliary epithelial cells. We report here that down-regulation of miR-98 and let-7 also coordinates epithelial expression of SOCS4 after C. parvum infection. Targeting of the SOCS4 3′ untranslated region by miR-98 or let-7 resulted in translational repression. Functional manipulation of miR98 caused reciprocal alterations in SOCS4 protein expression. Transfection of miR-98 precursor abolished C. parvwm-stimulated SOCS4 up-regulation. Moreover, expression of SOCS4 in epithelial cells showed an inhibitory effect on phosphorylation of signal transducers and activators of transcription proteins induced by C. parvum. These data suggest that miRNAs play an important role in the coordinated regulation of CIS and SOCS expression in epithelial cells in response to C. parvum infection.",
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