MicroRNas associated with shoulder tendon matrisome disorganization in glenohumeral arthritis

Finosh G. Thankam, Chandra S. Boosani, Matthew Dilisio, Nicholas E. Dietz, Devendra K. Agrawal

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The extracellular matrix (ECM) provides core support which is essential for the cell and tissue architectural development. The role of ECM in many pathological conditions has been well established and ECM-related abnormalities leading to serious consequences have been identified. Though much has been explored in regards to the role of ECM in soft tissue associated pathologies, very little is known about its role in inflammatory disorders in tendon. In this study, we performed microRNA (miRNA) expression analysis in the long head of the human shoulder biceps tendon to identify key genes whose expression was altered during inflammation in patients with glenohumeral arthritis. We identified differential regulation of matrix metalloproteinases (MMPs) that could be critical in collagen type replacement during tendinopathy. The miRNA profiling showed consistent results between the groups and revealed significant changes in the expression of seven different miRNAs in the inflamed tendons. Interestingly, all of these seven miRNAs were previously reported to have either a direct or indirect role in regulating the ECM organization in other pathological disorders. In addition, these miRNAs were also found to alter the expression levels of MMPs, which are the key matrix degrading enzymes associated with ECM-related abnormalities and pathologies. To our knowledge, this is the first report which identifies specific miRNAs associated with inflammation and the matrix reorganization in the tendons. Furthermore, the findings also support the potential role of these miRNAs in altering the collagen type ratio in the tendons during inflammation which is accompanied with differential expression of MMPs.

Original languageEnglish (US)
Article numbere0168077
JournalPLoS One
Volume11
Issue number12
DOIs
StatePublished - Dec 1 2016

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Tendons
arthritis
tendons
MicroRNAs
shoulders
microRNA
extracellular matrix
Arthritis
Extracellular Matrix
metalloproteinases
Matrix Metalloproteinases
inflammation
Pathology
Inflammation
collagen
Collagen
Tissue
Tendinopathy
Gene expression
gene expression

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

MicroRNas associated with shoulder tendon matrisome disorganization in glenohumeral arthritis. / Thankam, Finosh G.; Boosani, Chandra S.; Dilisio, Matthew; Dietz, Nicholas E.; Agrawal, Devendra K.

In: PLoS One, Vol. 11, No. 12, e0168077, 01.12.2016.

Research output: Contribution to journalArticle

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