Mir-133a in human circulating monocytes

A potential biomarker associated with postmenopausal osteoporosis

Yang Wang, Ling Li, Benjamin T. Moore, Xian Hao Peng, Xiang Fang, Joan M. Lappe, Robert R. Recker, Peng Xiao

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Background: Osteoporosis mainly occurs in postmenopausal women, which is characterized by low bone mineral density (BMD) due to unbalanced bone resorption by osteoclasts and formation by osteoblasts. Circulating monocytes play important roles in osteoclastogenesis by acting as osteoclast precursors and secreting osteoclastogenic factors, such as IL-1, IL-6 and TNF-α. MicroRNAs (miRNAs) have been implicated as important biomarkers in various diseases. The present study aimed to find significant miRNA biomarkers in human circulating monocytes underlying postmenopausal osteoporosis. Methodology/Principal Findings: We used ABI TaqMan® miRNA array followed by qRT-PCR validation in circulating monocytes to identify miRNA biomarkers in 10 high and 10 low BMD postmenopausal Caucasian women. MiR-133a was upregulated (P=0.007) in the low compared with the high BMD groups in the array analyses, which was also validated by qRT-PCR (P=0.044). We performed bioinformatic target gene analysis and found three potential osteoclast-related target genes, CXCL11, CXCR3 and SLC39A1. In addition, we performed Pearson correlation analyses between the expression levels of miR-133a and the three potential target genes in the 20 postmenopausal women. We did find negative correlations between miR-133a and all the three genes though not significant. Conclusions/Significance: This is the first in vivo miRNA expression analysis in human circulating monocytes to identify novel miRNA biomarkers underlying postmenopausal osteoporosis. Our results suggest that miR-133a in circulating monocytes is a potential biomarker for postmenopausal osteoporosis.

Original languageEnglish
Article numbere34641
JournalPLoS One
Volume7
Issue number4
DOIs
StatePublished - Apr 10 2012

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Postmenopausal Osteoporosis
osteoporosis
Biomarkers
MicroRNAs
microRNA
monocytes
Monocytes
biomarkers
osteoclasts
bone density
Bone
Osteoclasts
Genes
Bone Density
Minerals
Osteogenesis
genes
Polymerase Chain Reaction
bone resorption
osteoblasts

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Mir-133a in human circulating monocytes : A potential biomarker associated with postmenopausal osteoporosis. / Wang, Yang; Li, Ling; Moore, Benjamin T.; Peng, Xian Hao; Fang, Xiang; Lappe, Joan M.; Recker, Robert R.; Xiao, Peng.

In: PLoS One, Vol. 7, No. 4, e34641, 10.04.2012.

Research output: Contribution to journalArticle

Wang, Yang ; Li, Ling ; Moore, Benjamin T. ; Peng, Xian Hao ; Fang, Xiang ; Lappe, Joan M. ; Recker, Robert R. ; Xiao, Peng. / Mir-133a in human circulating monocytes : A potential biomarker associated with postmenopausal osteoporosis. In: PLoS One. 2012 ; Vol. 7, No. 4.
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