MiR-16 targets transcriptional corepressor SMRT and modulates NF-kappaB-regulated transactivation of interleukin-8 gene

Rui Zhou, Xiaoqing Li, Guoku Hu, Ai Yu Gong, Kristen M. Drescher, Xian-Ming Chen

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The signaling pathways associated with the Toll-like receptors (TLRs) and nuclear factor-kappaB (NF-κB) are essential to pro-inflammatory cytokine and chemokine expression, as well as initiating innate epithelial immune responses. The TLR/NF-κB signaling pathways must be stringently controlled through an intricate network of positive and negative regulatory elements. MicroRNAs (miRNAs) are non-coding small RNAs that regulate the stability and/or translation of protein-coding mRNAs. Herein we report that miR-16 promotes NF-κB-regulated transactivation of the IL-8 gene by suppression of the silencing mediator for retinoid and thyroid hormone receptor (SMRT). LPS stimulation activated miR-16 gene transcription in human monocytes (U937) and biliary epithelial cells (H69) through MAPK-dependent mechanisms. Transfection of cells with the miR-16 precursor promoted LPS-induced production of IL-8, IL-6, and IL-1α, without a significant effect on their RNA stability. Instead, an increase in NF-κB-regulated transactivation of the IL-8 gene was confirmed in cells following transfection of miR-16 precursor. Importantly, miR-16 targeted the 3′-untranslated region of SMRT and caused translational suppression of SMRT. LPS decreased SMRT expression via upregulation of miR-16. Moreover, functional manipulation of SMRT altered NF-κB-regulated transactivation of LPS-induced IL-8 expression. These data suggest that miR-16 targets SMRT and modulates NF-κB-regulated transactivation of the IL-8 gene.

Original languageEnglish
Article numbere30772
JournalPLoS One
Volume7
Issue number1
DOIs
StatePublished - Jan 24 2012

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Co-Repressor Proteins
transcription factor NF-kappa B
NF-kappa B
interleukin-8
transcriptional activation
Interleukin-8
Transcriptional Activation
Genes
Toll-Like Receptors
RNA Stability
genes
Transfection
transfection
Small Untranslated RNA
Thyroid Hormone Receptors
RNA
Retinoids
Protein Biosynthesis
3' Untranslated Regions
Gene Silencing

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

MiR-16 targets transcriptional corepressor SMRT and modulates NF-kappaB-regulated transactivation of interleukin-8 gene. / Zhou, Rui; Li, Xiaoqing; Hu, Guoku; Gong, Ai Yu; Drescher, Kristen M.; Chen, Xian-Ming.

In: PLoS One, Vol. 7, No. 1, e30772, 24.01.2012.

Research output: Contribution to journalArticle

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