MiR-422a as a potential cellular microRNA biomarker for postmenopausal osteoporosis

Zheng Cao, Benjamin T. Moore, Yang Wang, Xian Hao Peng, Joan M. Lappe, Robert R. Recker, Peng Xiao

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Background: MicroRNAs (miRNAs) are a class of short non-coding RNA molecules that regulate gene expression by targeting mRNAs. Recently, miRNAs have been shown to play important roles in the etiology of various diseases. However, little is known about their roles in the development of osteoporosis. Circulating monocytes are osteoclast precursors that also produce various factors important for osteoclastogenesis. Previously, we have identified a potential biomarker miR-133a in circulating monocytes for postmenopausal osteoporosis. In this study, we aimed to further identify significant miRNA biomarkers in human circulating monocytes underlying postmenopausal osteoporosis. Methodology/Principal Findings: We used ABI TaqMan miRNA array followed by qRT-PCR validation in human circulating monocytes from 10 high BMD and 10 low BMD postmenopausal Caucasian women to identify miRNA biomarkers. MiR-422a was up-regulated with marginal significance (P = 0.065) in the low compared with the high BMD group in the array analysis. However, a significant up-regulation of miR-422a was identified in the low BMD group by qRT-PCR analysis (P = 0.029). We also performed bioinformatic target gene analysis and found several potential target genes of miR-422a which are involved in osteoclastogenesis. Further qRT-PCR analyses of the target genes in the same study subjects showed that the expression of five of these genes (CBL, CD226, IGF1, PAG1, and TOB2) correlated negatively with miR-422a expression. Conclusions/Significance: Our study suggests that miR-422a in human circulating monocytes (osteoclast precursors) is a potential miRNA biomarker underlying postmenopausal osteoporosis.

Original languageEnglish
Article numbere97098
JournalPLoS One
Volume9
Issue number5
DOIs
StatePublished - May 12 2014

Fingerprint

Postmenopausal Osteoporosis
osteoporosis
Biomarkers
MicroRNAs
microRNA
biomarkers
monocytes
Monocytes
Genes
osteoclasts
Osteoclasts
Osteogenesis
Polymerase Chain Reaction
genes
Gene Expression
Untranslated RNA
Gene Targeting
Bioelectric potentials
Bioinformatics
Computational Biology

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

MiR-422a as a potential cellular microRNA biomarker for postmenopausal osteoporosis. / Cao, Zheng; Moore, Benjamin T.; Wang, Yang; Peng, Xian Hao; Lappe, Joan M.; Recker, Robert R.; Xiao, Peng.

In: PLoS One, Vol. 9, No. 5, e97098, 12.05.2014.

Research output: Contribution to journalArticle

Cao, Zheng ; Moore, Benjamin T. ; Wang, Yang ; Peng, Xian Hao ; Lappe, Joan M. ; Recker, Robert R. ; Xiao, Peng. / MiR-422a as a potential cellular microRNA biomarker for postmenopausal osteoporosis. In: PLoS One. 2014 ; Vol. 9, No. 5.
@article{d7aabbff1c1c46879604c49ad080cceb,
title = "MiR-422a as a potential cellular microRNA biomarker for postmenopausal osteoporosis",
abstract = "Background: MicroRNAs (miRNAs) are a class of short non-coding RNA molecules that regulate gene expression by targeting mRNAs. Recently, miRNAs have been shown to play important roles in the etiology of various diseases. However, little is known about their roles in the development of osteoporosis. Circulating monocytes are osteoclast precursors that also produce various factors important for osteoclastogenesis. Previously, we have identified a potential biomarker miR-133a in circulating monocytes for postmenopausal osteoporosis. In this study, we aimed to further identify significant miRNA biomarkers in human circulating monocytes underlying postmenopausal osteoporosis. Methodology/Principal Findings: We used ABI TaqMan miRNA array followed by qRT-PCR validation in human circulating monocytes from 10 high BMD and 10 low BMD postmenopausal Caucasian women to identify miRNA biomarkers. MiR-422a was up-regulated with marginal significance (P = 0.065) in the low compared with the high BMD group in the array analysis. However, a significant up-regulation of miR-422a was identified in the low BMD group by qRT-PCR analysis (P = 0.029). We also performed bioinformatic target gene analysis and found several potential target genes of miR-422a which are involved in osteoclastogenesis. Further qRT-PCR analyses of the target genes in the same study subjects showed that the expression of five of these genes (CBL, CD226, IGF1, PAG1, and TOB2) correlated negatively with miR-422a expression. Conclusions/Significance: Our study suggests that miR-422a in human circulating monocytes (osteoclast precursors) is a potential miRNA biomarker underlying postmenopausal osteoporosis.",
author = "Zheng Cao and Moore, {Benjamin T.} and Yang Wang and Peng, {Xian Hao} and Lappe, {Joan M.} and Recker, {Robert R.} and Peng Xiao",
year = "2014",
month = "5",
day = "12",
doi = "10.1371/journal.pone.0097098",
language = "English",
volume = "9",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",

}

TY - JOUR

T1 - MiR-422a as a potential cellular microRNA biomarker for postmenopausal osteoporosis

AU - Cao, Zheng

AU - Moore, Benjamin T.

AU - Wang, Yang

AU - Peng, Xian Hao

AU - Lappe, Joan M.

AU - Recker, Robert R.

AU - Xiao, Peng

PY - 2014/5/12

Y1 - 2014/5/12

N2 - Background: MicroRNAs (miRNAs) are a class of short non-coding RNA molecules that regulate gene expression by targeting mRNAs. Recently, miRNAs have been shown to play important roles in the etiology of various diseases. However, little is known about their roles in the development of osteoporosis. Circulating monocytes are osteoclast precursors that also produce various factors important for osteoclastogenesis. Previously, we have identified a potential biomarker miR-133a in circulating monocytes for postmenopausal osteoporosis. In this study, we aimed to further identify significant miRNA biomarkers in human circulating monocytes underlying postmenopausal osteoporosis. Methodology/Principal Findings: We used ABI TaqMan miRNA array followed by qRT-PCR validation in human circulating monocytes from 10 high BMD and 10 low BMD postmenopausal Caucasian women to identify miRNA biomarkers. MiR-422a was up-regulated with marginal significance (P = 0.065) in the low compared with the high BMD group in the array analysis. However, a significant up-regulation of miR-422a was identified in the low BMD group by qRT-PCR analysis (P = 0.029). We also performed bioinformatic target gene analysis and found several potential target genes of miR-422a which are involved in osteoclastogenesis. Further qRT-PCR analyses of the target genes in the same study subjects showed that the expression of five of these genes (CBL, CD226, IGF1, PAG1, and TOB2) correlated negatively with miR-422a expression. Conclusions/Significance: Our study suggests that miR-422a in human circulating monocytes (osteoclast precursors) is a potential miRNA biomarker underlying postmenopausal osteoporosis.

AB - Background: MicroRNAs (miRNAs) are a class of short non-coding RNA molecules that regulate gene expression by targeting mRNAs. Recently, miRNAs have been shown to play important roles in the etiology of various diseases. However, little is known about their roles in the development of osteoporosis. Circulating monocytes are osteoclast precursors that also produce various factors important for osteoclastogenesis. Previously, we have identified a potential biomarker miR-133a in circulating monocytes for postmenopausal osteoporosis. In this study, we aimed to further identify significant miRNA biomarkers in human circulating monocytes underlying postmenopausal osteoporosis. Methodology/Principal Findings: We used ABI TaqMan miRNA array followed by qRT-PCR validation in human circulating monocytes from 10 high BMD and 10 low BMD postmenopausal Caucasian women to identify miRNA biomarkers. MiR-422a was up-regulated with marginal significance (P = 0.065) in the low compared with the high BMD group in the array analysis. However, a significant up-regulation of miR-422a was identified in the low BMD group by qRT-PCR analysis (P = 0.029). We also performed bioinformatic target gene analysis and found several potential target genes of miR-422a which are involved in osteoclastogenesis. Further qRT-PCR analyses of the target genes in the same study subjects showed that the expression of five of these genes (CBL, CD226, IGF1, PAG1, and TOB2) correlated negatively with miR-422a expression. Conclusions/Significance: Our study suggests that miR-422a in human circulating monocytes (osteoclast precursors) is a potential miRNA biomarker underlying postmenopausal osteoporosis.

UR - http://www.scopus.com/inward/record.url?scp=84901276445&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901276445&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0097098

DO - 10.1371/journal.pone.0097098

M3 - Article

C2 - 24820117

AN - SCOPUS:84901276445

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 5

M1 - e97098

ER -