Modification of the double-disk test for detection of Enterobacteriaceae producing extended-spectrum and AmpC β-lactamases

J. D.D. Pitout; M. D. Reisbig; E. C. Venter; D. L. Church; N. D. Hanson

doi:10.1128/JCM.41.8.3933-3935.2003

Modification of the double-disk test for detection of Enterobacteriaceae producing extended-spectrum and AmpC β-lactamases

J. D.D. Pitout, M. D. Reisbig, E. C. Venter, D. L. Church, N. D. Hanson

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

Detection of extended-spectrum β-lactamases (ESBLs) in AmpC-producing Enterobacteriaceae is problematic. A modification of the double-disk test (MDDT) has been developed for successful detection of ESBLs in gram-negative bacilli producing well-characterized β-lactamases as well as 212 clinical isolates of Enterobacter cloacae, Enterobacter aerogenes, Serratia marcescens, and Citrobacter freundii. MDDT accurately differentiated between ESBL producers and derepressed chromosomal AmpC mutants. MDDT provides a cost-effective alternative approach for clinical microbiology laboratories for routine susceptibility testing with simultaneous detection of ESBLs in Enterobacteriaceae.

Original language	English (US)
Pages (from-to)	3933-3935
Number of pages	3
Journal	Journal of clinical microbiology
Volume	41
Issue number	8
DOIs	https://doi.org/10.1128/JCM.41.8.3933-3935.2003
State	Published - Aug 1 2003

All Science Journal Classification (ASJC) codes

Microbiology (medical)

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abstract = "Detection of extended-spectrum β-lactamases (ESBLs) in AmpC-producing Enterobacteriaceae is problematic. A modification of the double-disk test (MDDT) has been developed for successful detection of ESBLs in gram-negative bacilli producing well-characterized β-lactamases as well as 212 clinical isolates of Enterobacter cloacae, Enterobacter aerogenes, Serratia marcescens, and Citrobacter freundii. MDDT accurately differentiated between ESBL producers and derepressed chromosomal AmpC mutants. MDDT provides a cost-effective alternative approach for clinical microbiology laboratories for routine susceptibility testing with simultaneous detection of ESBLs in Enterobacteriaceae.",

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AU - Pitout, J. D.D.

AU - Reisbig, M. D.

AU - Venter, E. C.

AU - Church, D. L.

AU - Hanson, N. D.

PY - 2003/8/1

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AB - Detection of extended-spectrum β-lactamases (ESBLs) in AmpC-producing Enterobacteriaceae is problematic. A modification of the double-disk test (MDDT) has been developed for successful detection of ESBLs in gram-negative bacilli producing well-characterized β-lactamases as well as 212 clinical isolates of Enterobacter cloacae, Enterobacter aerogenes, Serratia marcescens, and Citrobacter freundii. MDDT accurately differentiated between ESBL producers and derepressed chromosomal AmpC mutants. MDDT provides a cost-effective alternative approach for clinical microbiology laboratories for routine susceptibility testing with simultaneous detection of ESBLs in Enterobacteriaceae.

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Modification of the double-disk test for detection of Enterobacteriaceae producing extended-spectrum and AmpC β-lactamases

Abstract

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