Abstract
Detection of extended-spectrum β-lactamases (ESBLs) in AmpC-producing Enterobacteriaceae is problematic. A modification of the double-disk test (MDDT) has been developed for successful detection of ESBLs in gram-negative bacilli producing well-characterized β-lactamases as well as 212 clinical isolates of Enterobacter cloacae, Enterobacter aerogenes, Serratia marcescens, and Citrobacter freundii. MDDT accurately differentiated between ESBL producers and derepressed chromosomal AmpC mutants. MDDT provides a cost-effective alternative approach for clinical microbiology laboratories for routine susceptibility testing with simultaneous detection of ESBLs in Enterobacteriaceae.
| Original language | English |
|---|---|
| Pages (from-to) | 3933-3935 |
| Number of pages | 3 |
| Journal | Journal of Clinical Microbiology |
| Volume | 41 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 1 2003 |
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All Science Journal Classification (ASJC) codes
- Microbiology (medical)
- Microbiology
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Modification of the double-disk test for detection of Enterobacteriaceae producing extended-spectrum and AmpC β-lactamases. / Pitout, J. D D; Reisbig, Mark; Venter, E. C.; Church, D. L.; Hanson, Nancy D.
In: Journal of Clinical Microbiology, Vol. 41, No. 8, 01.08.2003, p. 3933-3935.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Modification of the double-disk test for detection of Enterobacteriaceae producing extended-spectrum and AmpC β-lactamases
AU - Pitout, J. D D
AU - Reisbig, Mark
AU - Venter, E. C.
AU - Church, D. L.
AU - Hanson, Nancy D.
PY - 2003/8/1
Y1 - 2003/8/1
N2 - Detection of extended-spectrum β-lactamases (ESBLs) in AmpC-producing Enterobacteriaceae is problematic. A modification of the double-disk test (MDDT) has been developed for successful detection of ESBLs in gram-negative bacilli producing well-characterized β-lactamases as well as 212 clinical isolates of Enterobacter cloacae, Enterobacter aerogenes, Serratia marcescens, and Citrobacter freundii. MDDT accurately differentiated between ESBL producers and derepressed chromosomal AmpC mutants. MDDT provides a cost-effective alternative approach for clinical microbiology laboratories for routine susceptibility testing with simultaneous detection of ESBLs in Enterobacteriaceae.
AB - Detection of extended-spectrum β-lactamases (ESBLs) in AmpC-producing Enterobacteriaceae is problematic. A modification of the double-disk test (MDDT) has been developed for successful detection of ESBLs in gram-negative bacilli producing well-characterized β-lactamases as well as 212 clinical isolates of Enterobacter cloacae, Enterobacter aerogenes, Serratia marcescens, and Citrobacter freundii. MDDT accurately differentiated between ESBL producers and derepressed chromosomal AmpC mutants. MDDT provides a cost-effective alternative approach for clinical microbiology laboratories for routine susceptibility testing with simultaneous detection of ESBLs in Enterobacteriaceae.
UR - http://www.scopus.com/inward/record.url?scp=0042023190&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0042023190&partnerID=8YFLogxK
U2 - 10.1128/JCM.41.8.3933-3935.2003
DO - 10.1128/JCM.41.8.3933-3935.2003
M3 - Article
C2 - 12904422
AN - SCOPUS:0042023190
VL - 41
SP - 3933
EP - 3935
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 8
ER -