Modulation by Topiramate of AMPA and Kainate Mediated Calcium Influx in Cultured Cerebral Cortical, Hippocampal and Cerebellar Neurons

Claus F. Poulsen, Timothy Simeone, Thomas E. Maar, Virginia Smith-Swintosky, H. Steven White, Arne Schousboe

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The effect of the antiepileptic drug topiramate on Ca2+ uptake through (RS)-2-amino-3-(3-hydroxy-5-methylisoxazol-4-yl)propionate (AMPA) and kainate (KA) receptors was investigated in different cell culture systems consisting of neurons from the cerebral cortex, hippocampus, and cerebellum. Ca2+ influx was assayed using a fluorescent Ca2+ chelator to monitor changes in the intracellular Ca2+ concentration or cobalt staining to assess the effect of topiramate on Ca2+-permeable AMPA/ KA receptors. In all types of neuronal cultures studied, AMPA and KA were found to elicit an influx of Ca2+ in a subset of the neuronal population. Topiramate, at concentrations of 30 and 100 μM, inhibited Ca2+ influx by up to 60%. Modulation of AMPA and KA-evoked Ca2+ influx may contribute to both the antiepileptic and neuroprotective properties of topiramate.

Original languageEnglish
Pages (from-to)275-282
Number of pages8
JournalNeurochemical Research
Volume29
Issue number1
DOIs
StatePublished - Jan 2004

Fingerprint

alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Kainic Acid
Neurons
Modulation
Calcium
Kainic Acid Receptors
AMPA Receptors
Anticonvulsants
Propionates
Chelating Agents
Cobalt
Cell culture
Cerebral Cortex
Cerebellum
Hippocampus
Cell Culture Techniques
Staining and Labeling
topiramate
Population

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry

Cite this

Modulation by Topiramate of AMPA and Kainate Mediated Calcium Influx in Cultured Cerebral Cortical, Hippocampal and Cerebellar Neurons. / Poulsen, Claus F.; Simeone, Timothy; Maar, Thomas E.; Smith-Swintosky, Virginia; White, H. Steven; Schousboe, Arne.

In: Neurochemical Research, Vol. 29, No. 1, 01.2004, p. 275-282.

Research output: Contribution to journalArticle

Poulsen, Claus F. ; Simeone, Timothy ; Maar, Thomas E. ; Smith-Swintosky, Virginia ; White, H. Steven ; Schousboe, Arne. / Modulation by Topiramate of AMPA and Kainate Mediated Calcium Influx in Cultured Cerebral Cortical, Hippocampal and Cerebellar Neurons. In: Neurochemical Research. 2004 ; Vol. 29, No. 1. pp. 275-282.
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