Abstract
The effect of the antiepileptic drug topiramate on Ca2+ uptake through (RS)-2-amino-3-(3-hydroxy-5-methylisoxazol-4-yl)propionate (AMPA) and kainate (KA) receptors was investigated in different cell culture systems consisting of neurons from the cerebral cortex, hippocampus, and cerebellum. Ca2+ influx was assayed using a fluorescent Ca2+ chelator to monitor changes in the intracellular Ca2+ concentration or cobalt staining to assess the effect of topiramate on Ca2+-permeable AMPA/ KA receptors. In all types of neuronal cultures studied, AMPA and KA were found to elicit an influx of Ca2+ in a subset of the neuronal population. Topiramate, at concentrations of 30 and 100 μM, inhibited Ca2+ influx by up to 60%. Modulation of AMPA and KA-evoked Ca2+ influx may contribute to both the antiepileptic and neuroprotective properties of topiramate.
| Original language | English |
|---|---|
| Pages (from-to) | 275-282 |
| Number of pages | 8 |
| Journal | Neurochemical Research |
| Volume | 29 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2004 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
- Biochemistry
Cite this
Modulation by Topiramate of AMPA and Kainate Mediated Calcium Influx in Cultured Cerebral Cortical, Hippocampal and Cerebellar Neurons. / Poulsen, Claus F.; Simeone, Timothy; Maar, Thomas E.; Smith-Swintosky, Virginia; White, H. Steven; Schousboe, Arne.
In: Neurochemical Research, Vol. 29, No. 1, 01.2004, p. 275-282.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Modulation by Topiramate of AMPA and Kainate Mediated Calcium Influx in Cultured Cerebral Cortical, Hippocampal and Cerebellar Neurons
AU - Poulsen, Claus F.
AU - Simeone, Timothy
AU - Maar, Thomas E.
AU - Smith-Swintosky, Virginia
AU - White, H. Steven
AU - Schousboe, Arne
PY - 2004/1
Y1 - 2004/1
N2 - The effect of the antiepileptic drug topiramate on Ca2+ uptake through (RS)-2-amino-3-(3-hydroxy-5-methylisoxazol-4-yl)propionate (AMPA) and kainate (KA) receptors was investigated in different cell culture systems consisting of neurons from the cerebral cortex, hippocampus, and cerebellum. Ca2+ influx was assayed using a fluorescent Ca2+ chelator to monitor changes in the intracellular Ca2+ concentration or cobalt staining to assess the effect of topiramate on Ca2+-permeable AMPA/ KA receptors. In all types of neuronal cultures studied, AMPA and KA were found to elicit an influx of Ca2+ in a subset of the neuronal population. Topiramate, at concentrations of 30 and 100 μM, inhibited Ca2+ influx by up to 60%. Modulation of AMPA and KA-evoked Ca2+ influx may contribute to both the antiepileptic and neuroprotective properties of topiramate.
AB - The effect of the antiepileptic drug topiramate on Ca2+ uptake through (RS)-2-amino-3-(3-hydroxy-5-methylisoxazol-4-yl)propionate (AMPA) and kainate (KA) receptors was investigated in different cell culture systems consisting of neurons from the cerebral cortex, hippocampus, and cerebellum. Ca2+ influx was assayed using a fluorescent Ca2+ chelator to monitor changes in the intracellular Ca2+ concentration or cobalt staining to assess the effect of topiramate on Ca2+-permeable AMPA/ KA receptors. In all types of neuronal cultures studied, AMPA and KA were found to elicit an influx of Ca2+ in a subset of the neuronal population. Topiramate, at concentrations of 30 and 100 μM, inhibited Ca2+ influx by up to 60%. Modulation of AMPA and KA-evoked Ca2+ influx may contribute to both the antiepileptic and neuroprotective properties of topiramate.
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UR - http://www.scopus.com/inward/citedby.url?scp=0442328037&partnerID=8YFLogxK
U2 - 10.1023/B:NERE.0000010456.92887.3b
DO - 10.1023/B:NERE.0000010456.92887.3b
M3 - Article
C2 - 14992287
AN - SCOPUS:0442328037
VL - 29
SP - 275
EP - 282
JO - Neurochemical Research
JF - Neurochemical Research
SN - 0364-3190
IS - 1
ER -