Abstract
Neuronal properties were investigated through patch-clamp recording in situ in surgical specimens of the human lateral amygdala (LA) obtained from patients with intractable temporal lobe epilepsy. Projection neurons displayed spiny dendrites, action potentials with varying degree of frequency adaptation, and an inwardly rectifying K+ (Kir) conductance coupled to GABAB receptors. In interneurons, dendrites were spineless or sparsely spiny, action potentials were shorter than those in projection neurons and often occurred spontaneously, and GABAB receptor-mediated responses were lacking. Single-cell RT-PCR demonstrated expression of Kir channel subunits Kir3.1 and Kir3.2 and of vesicular glutamate transporters VGLUT1 and VGLUT2 in projection neurons. It is concluded that projection neurons and interneurons of the human LA can be distinguished based upon morphological, electrophysiological, and molecular biological criteria. The most striking difference relates to the expression of postsynaptic GABAB receptors coupled to Kir3 channels in projection neurons and the lack of functional GABAB receptors in interneurons.
| Original language | English |
|---|---|
| Pages (from-to) | 210-217 |
| Number of pages | 8 |
| Journal | Molecular and Cellular Neurosciences |
| Volume | 31 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2006 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cellular and Molecular Neuroscience
- Developmental Neuroscience
Cite this
Molecular and functional properties of neurons in the human lateral amygdala. / Hüttmann, Kerstin; Yilmazer-Hanke, Deniz; Seifert, Gerald; Schramm, Johannes; Pape, Hans Christian; Steinhäuser, Christian.
In: Molecular and Cellular Neurosciences, Vol. 31, No. 2, 02.2006, p. 210-217.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular and functional properties of neurons in the human lateral amygdala
AU - Hüttmann, Kerstin
AU - Yilmazer-Hanke, Deniz
AU - Seifert, Gerald
AU - Schramm, Johannes
AU - Pape, Hans Christian
AU - Steinhäuser, Christian
PY - 2006/2
Y1 - 2006/2
N2 - Neuronal properties were investigated through patch-clamp recording in situ in surgical specimens of the human lateral amygdala (LA) obtained from patients with intractable temporal lobe epilepsy. Projection neurons displayed spiny dendrites, action potentials with varying degree of frequency adaptation, and an inwardly rectifying K+ (Kir) conductance coupled to GABAB receptors. In interneurons, dendrites were spineless or sparsely spiny, action potentials were shorter than those in projection neurons and often occurred spontaneously, and GABAB receptor-mediated responses were lacking. Single-cell RT-PCR demonstrated expression of Kir channel subunits Kir3.1 and Kir3.2 and of vesicular glutamate transporters VGLUT1 and VGLUT2 in projection neurons. It is concluded that projection neurons and interneurons of the human LA can be distinguished based upon morphological, electrophysiological, and molecular biological criteria. The most striking difference relates to the expression of postsynaptic GABAB receptors coupled to Kir3 channels in projection neurons and the lack of functional GABAB receptors in interneurons.
AB - Neuronal properties were investigated through patch-clamp recording in situ in surgical specimens of the human lateral amygdala (LA) obtained from patients with intractable temporal lobe epilepsy. Projection neurons displayed spiny dendrites, action potentials with varying degree of frequency adaptation, and an inwardly rectifying K+ (Kir) conductance coupled to GABAB receptors. In interneurons, dendrites were spineless or sparsely spiny, action potentials were shorter than those in projection neurons and often occurred spontaneously, and GABAB receptor-mediated responses were lacking. Single-cell RT-PCR demonstrated expression of Kir channel subunits Kir3.1 and Kir3.2 and of vesicular glutamate transporters VGLUT1 and VGLUT2 in projection neurons. It is concluded that projection neurons and interneurons of the human LA can be distinguished based upon morphological, electrophysiological, and molecular biological criteria. The most striking difference relates to the expression of postsynaptic GABAB receptors coupled to Kir3 channels in projection neurons and the lack of functional GABAB receptors in interneurons.
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UR - http://www.scopus.com/inward/citedby.url?scp=32244444735&partnerID=8YFLogxK
U2 - 10.1016/j.mcn.2005.09.011
DO - 10.1016/j.mcn.2005.09.011
M3 - Article
C2 - 16214367
AN - SCOPUS:32244444735
VL - 31
SP - 210
EP - 217
JO - Molecular and Cellular Neurosciences
JF - Molecular and Cellular Neurosciences
SN - 1044-7431
IS - 2
ER -