Molecular characterization of a multiply resistant Klebsiella pneumoniae encoding ESBLs and a plasmid-mediated AmpC

Nancy D. Hanson; Kenneth S. Thomson; Ellen Smith Moland; Christine C. Sanders; Gerald Berthold; Robert G. Penn

doi:10.1093/jac/44.3.377

Molecular characterization of a multiply resistant Klebsiella pneumoniae encoding ESBLs and a plasmid-mediated AmpC

Nancy D. Hanson, Kenneth S. Thomson, Ellen Smith Moland, Christine C. Sanders, Gerald Berthold, Robert G. Penn

Department of Medical Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Organisms encoding multiple antibiotic resistance genes are becoming increasingly prevalent. In this report we describe a multiply resistant Klebsiella pneumoniae which possesses at least five different β-lactamase genes. Isoelectric focusing, polymerase chain reaction and restriction fragment length polymorphism analysis identified TEM-1, multiple SHVs, OXA-9 and a plasmid-mediated ampC β-lactamase. Furthermore, Southern analysis and conjugation experiments established that most of the resistance genes were encoded on one large transferable plasmid. This report demonstrates the complexity of multiply resistant organisms.

Original language	English (US)
Pages (from-to)	377-380
Number of pages	4
Journal	Journal of Antimicrobial Chemotherapy
Volume	44
Issue number	3
DOIs	https://doi.org/10.1093/jac/44.3.377
State	Published - 1999

All Science Journal Classification (ASJC) codes

Pharmacology
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)

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Molecular characterization of a multiply resistant Klebsiella pneumoniae encoding ESBLs and a plasmid-mediated AmpC. / Hanson, Nancy D.; Thomson, Kenneth S.; Moland, Ellen Smith; Sanders, Christine C.; Berthold, Gerald; Penn, Robert G.

In: Journal of Antimicrobial Chemotherapy, Vol. 44, No. 3, 1999, p. 377-380.

Research output: Contribution to journal › Article › peer-review

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AU - Penn, Robert G.

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AB - Organisms encoding multiple antibiotic resistance genes are becoming increasingly prevalent. In this report we describe a multiply resistant Klebsiella pneumoniae which possesses at least five different β-lactamase genes. Isoelectric focusing, polymerase chain reaction and restriction fragment length polymorphism analysis identified TEM-1, multiple SHVs, OXA-9 and a plasmid-mediated ampC β-lactamase. Furthermore, Southern analysis and conjugation experiments established that most of the resistance genes were encoded on one large transferable plasmid. This report demonstrates the complexity of multiply resistant organisms.

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Molecular characterization of a multiply resistant Klebsiella pneumoniae encoding ESBLs and a plasmid-mediated AmpC

Abstract

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