Molecular characterization of a multiply resistant Klebsiella pneumoniae encoding ESBLs and a plasmid-mediated AmpC

Nancy D. Hanson, Kenneth S. Thomson, Ellen Smith Moland, Christine C. Sanders, Gerald Berthold, Robert G. Penn

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Organisms encoding multiple antibiotic resistance genes are becoming increasingly prevalent. In this report we describe a multiply resistant Klebsiella pneumoniae which possesses at least five different β-lactamase genes. Isoelectric focusing, polymerase chain reaction and restriction fragment length polymorphism analysis identified TEM-1, multiple SHVs, OXA-9 and a plasmid-mediated ampC β-lactamase. Furthermore, Southern analysis and conjugation experiments established that most of the resistance genes were encoded on one large transferable plasmid. This report demonstrates the complexity of multiply resistant organisms.

Original languageEnglish
Pages (from-to)377-380
Number of pages4
JournalJournal of Antimicrobial Chemotherapy
Volume44
Issue number3
DOIs
StatePublished - 1999

Fingerprint

Klebsiella pneumoniae
Plasmids
Genes
Isoelectric Focusing
Microbial Drug Resistance
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology

Cite this

Molecular characterization of a multiply resistant Klebsiella pneumoniae encoding ESBLs and a plasmid-mediated AmpC. / Hanson, Nancy D.; Thomson, Kenneth S.; Moland, Ellen Smith; Sanders, Christine C.; Berthold, Gerald; Penn, Robert G.

In: Journal of Antimicrobial Chemotherapy, Vol. 44, No. 3, 1999, p. 377-380.

Research output: Contribution to journalArticle

Hanson, Nancy D. ; Thomson, Kenneth S. ; Moland, Ellen Smith ; Sanders, Christine C. ; Berthold, Gerald ; Penn, Robert G. / Molecular characterization of a multiply resistant Klebsiella pneumoniae encoding ESBLs and a plasmid-mediated AmpC. In: Journal of Antimicrobial Chemotherapy. 1999 ; Vol. 44, No. 3. pp. 377-380.
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