Abstract
Organisms encoding multiple antibiotic resistance genes are becoming increasingly prevalent. In this report we describe a multiply resistant Klebsiella pneumoniae which possesses at least five different β-lactamase genes. Isoelectric focusing, polymerase chain reaction and restriction fragment length polymorphism analysis identified TEM-1, multiple SHVs, OXA-9 and a plasmid-mediated ampC β-lactamase. Furthermore, Southern analysis and conjugation experiments established that most of the resistance genes were encoded on one large transferable plasmid. This report demonstrates the complexity of multiply resistant organisms.
| Original language | English |
|---|---|
| Pages (from-to) | 377-380 |
| Number of pages | 4 |
| Journal | Journal of Antimicrobial Chemotherapy |
| Volume | 44 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1999 |
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All Science Journal Classification (ASJC) codes
- Pharmacology
- Microbiology
Cite this
Molecular characterization of a multiply resistant Klebsiella pneumoniae encoding ESBLs and a plasmid-mediated AmpC. / Hanson, Nancy D.; Thomson, Kenneth S.; Moland, Ellen Smith; Sanders, Christine C.; Berthold, Gerald; Penn, Robert G.
In: Journal of Antimicrobial Chemotherapy, Vol. 44, No. 3, 1999, p. 377-380.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular characterization of a multiply resistant Klebsiella pneumoniae encoding ESBLs and a plasmid-mediated AmpC
AU - Hanson, Nancy D.
AU - Thomson, Kenneth S.
AU - Moland, Ellen Smith
AU - Sanders, Christine C.
AU - Berthold, Gerald
AU - Penn, Robert G.
PY - 1999
Y1 - 1999
N2 - Organisms encoding multiple antibiotic resistance genes are becoming increasingly prevalent. In this report we describe a multiply resistant Klebsiella pneumoniae which possesses at least five different β-lactamase genes. Isoelectric focusing, polymerase chain reaction and restriction fragment length polymorphism analysis identified TEM-1, multiple SHVs, OXA-9 and a plasmid-mediated ampC β-lactamase. Furthermore, Southern analysis and conjugation experiments established that most of the resistance genes were encoded on one large transferable plasmid. This report demonstrates the complexity of multiply resistant organisms.
AB - Organisms encoding multiple antibiotic resistance genes are becoming increasingly prevalent. In this report we describe a multiply resistant Klebsiella pneumoniae which possesses at least five different β-lactamase genes. Isoelectric focusing, polymerase chain reaction and restriction fragment length polymorphism analysis identified TEM-1, multiple SHVs, OXA-9 and a plasmid-mediated ampC β-lactamase. Furthermore, Southern analysis and conjugation experiments established that most of the resistance genes were encoded on one large transferable plasmid. This report demonstrates the complexity of multiply resistant organisms.
UR - http://www.scopus.com/inward/record.url?scp=0032836269&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032836269&partnerID=8YFLogxK
U2 - 10.1093/jac/44.3.377
DO - 10.1093/jac/44.3.377
M3 - Article
C2 - 10511405
AN - SCOPUS:0032836269
VL - 44
SP - 377
EP - 380
JO - Journal of Antimicrobial Chemotherapy
JF - Journal of Antimicrobial Chemotherapy
SN - 0305-7453
IS - 3
ER -