Molecular epidemiological analysis of Escherichia coli sequence type ST131 (O25

H4) and bla CTX-M-15among extended-spectrum-β- lactamase-producing E. coli from the United States, 2000 to 2009

James R. Johnson, Carl Urban, Scott J. Weissman, James H. Jorgensen, James S. Lewis, Glen Hansen, Paul H. Edelstein, Ari Robicsek, Timothy Cleary, Javier Adachi, David Paterson, John Quinn, Nancy D. Hanson, Brian D. Johnston, Connie Clabots, Michael A. Kuskowski, Robert L. Bergsbaken, Thomas M. Hooton, Michelle Hulse, Karen Lolans & 3 others Rob Owens, Elizabeth Palavecino, Karen Vigil

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Escherichia coli sequence type ST131 (from phylogenetic group B2), often carrying the extended-spectrum-β-lactamase (ESBL) gene bla CTX-M-15, is an emerging globally disseminated pathogen that has received comparatively little attention in the United States. Accordingly, a convenience sample of 351 ESBL-producing E. coli isolates from 15 U.S. centers (collected in 2000 to 2009) underwent PCR-based phylotyping and detection of ST131 and bla CTX-M-15. A total of 200 isolates, comprising 4 groups of 50 isolates each that were (i) bla CTX-M-15 negative non-ST131, (ii) bla CTX-M-15 positive non-ST131, (iii) bla CTX-M-15 negative ST131, or (iv) bla CTX-M-15 positive ST131, also underwent virulence genotyping, antimicrobial susceptibility testing, and pulsed-field gel electrophoresis (PFGE). Overall, 201 (57%) isolates exhibited bla CTX-M-15, whereas 165 (47%) were ST131. ST131 accounted for 56% of bla CTX-M-15-positive-versus 35% of bla CTX-M-15-negative isolates (PCTX-M-15 status, with groups A (bla CTX-M-15-positive isolates) andD(bla CTX-M-15- negative isolates) predominating. Both bla CTX-M-15 and ST131 occurred at all participating centers, were recovered from children and adults, increased significantly in prevalence post-2003, and were associated with molecularly inferred virulence. Compared with non- ST131 isolates, ST131 isolates had higher virulence scores, distinctive virulence profiles, and more-homogeneous PFGE profiles. bla CTX-M-15 was associated with extensive antimicrobial resistance and ST131 with fluoroquinolone resistance. Thus, E. coli ST131 and bla CTX-M-15 are emergent, widely distributed, and predominant among ESBL-positive E. coli strains in the United States, among children and adults alike. Enhanced virulence and antimicrobial resistance have likely promoted the epidemiological success of these emerging public health threats.

Original languageEnglish
Pages (from-to)2364-2370
Number of pages7
JournalAntimicrobial Agents and Chemotherapy
Volume56
Issue number5
DOIs
StatePublished - May 2012

Fingerprint

Escherichia coli
Virulence
Pulsed Field Gel Electrophoresis
galantide
Fluoroquinolones
Public Health
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

Cite this

Molecular epidemiological analysis of Escherichia coli sequence type ST131 (O25 : H4) and bla CTX-M-15among extended-spectrum-β- lactamase-producing E. coli from the United States, 2000 to 2009. / Johnson, James R.; Urban, Carl; Weissman, Scott J.; Jorgensen, James H.; Lewis, James S.; Hansen, Glen; Edelstein, Paul H.; Robicsek, Ari; Cleary, Timothy; Adachi, Javier; Paterson, David; Quinn, John; Hanson, Nancy D.; Johnston, Brian D.; Clabots, Connie; Kuskowski, Michael A.; Bergsbaken, Robert L.; Hooton, Thomas M.; Hulse, Michelle; Lolans, Karen; Owens, Rob; Palavecino, Elizabeth; Vigil, Karen.

In: Antimicrobial Agents and Chemotherapy, Vol. 56, No. 5, 05.2012, p. 2364-2370.

Research output: Contribution to journalArticle

Johnson, JR, Urban, C, Weissman, SJ, Jorgensen, JH, Lewis, JS, Hansen, G, Edelstein, PH, Robicsek, A, Cleary, T, Adachi, J, Paterson, D, Quinn, J, Hanson, ND, Johnston, BD, Clabots, C, Kuskowski, MA, Bergsbaken, RL, Hooton, TM, Hulse, M, Lolans, K, Owens, R, Palavecino, E & Vigil, K 2012, 'Molecular epidemiological analysis of Escherichia coli sequence type ST131 (O25: H4) and bla CTX-M-15among extended-spectrum-β- lactamase-producing E. coli from the United States, 2000 to 2009', Antimicrobial Agents and Chemotherapy, vol. 56, no. 5, pp. 2364-2370. https://doi.org/10.1128/AAC.05824-11
Johnson, James R. ; Urban, Carl ; Weissman, Scott J. ; Jorgensen, James H. ; Lewis, James S. ; Hansen, Glen ; Edelstein, Paul H. ; Robicsek, Ari ; Cleary, Timothy ; Adachi, Javier ; Paterson, David ; Quinn, John ; Hanson, Nancy D. ; Johnston, Brian D. ; Clabots, Connie ; Kuskowski, Michael A. ; Bergsbaken, Robert L. ; Hooton, Thomas M. ; Hulse, Michelle ; Lolans, Karen ; Owens, Rob ; Palavecino, Elizabeth ; Vigil, Karen. / Molecular epidemiological analysis of Escherichia coli sequence type ST131 (O25 : H4) and bla CTX-M-15among extended-spectrum-β- lactamase-producing E. coli from the United States, 2000 to 2009. In: Antimicrobial Agents and Chemotherapy. 2012 ; Vol. 56, No. 5. pp. 2364-2370.
@article{e98b9f0588d1411b9db5518e344ded06,
title = "Molecular epidemiological analysis of Escherichia coli sequence type ST131 (O25: H4) and bla CTX-M-15among extended-spectrum-β- lactamase-producing E. coli from the United States, 2000 to 2009",
abstract = "Escherichia coli sequence type ST131 (from phylogenetic group B2), often carrying the extended-spectrum-β-lactamase (ESBL) gene bla CTX-M-15, is an emerging globally disseminated pathogen that has received comparatively little attention in the United States. Accordingly, a convenience sample of 351 ESBL-producing E. coli isolates from 15 U.S. centers (collected in 2000 to 2009) underwent PCR-based phylotyping and detection of ST131 and bla CTX-M-15. A total of 200 isolates, comprising 4 groups of 50 isolates each that were (i) bla CTX-M-15 negative non-ST131, (ii) bla CTX-M-15 positive non-ST131, (iii) bla CTX-M-15 negative ST131, or (iv) bla CTX-M-15 positive ST131, also underwent virulence genotyping, antimicrobial susceptibility testing, and pulsed-field gel electrophoresis (PFGE). Overall, 201 (57{\%}) isolates exhibited bla CTX-M-15, whereas 165 (47{\%}) were ST131. ST131 accounted for 56{\%} of bla CTX-M-15-positive-versus 35{\%} of bla CTX-M-15-negative isolates (PCTX-M-15 status, with groups A (bla CTX-M-15-positive isolates) andD(bla CTX-M-15- negative isolates) predominating. Both bla CTX-M-15 and ST131 occurred at all participating centers, were recovered from children and adults, increased significantly in prevalence post-2003, and were associated with molecularly inferred virulence. Compared with non- ST131 isolates, ST131 isolates had higher virulence scores, distinctive virulence profiles, and more-homogeneous PFGE profiles. bla CTX-M-15 was associated with extensive antimicrobial resistance and ST131 with fluoroquinolone resistance. Thus, E. coli ST131 and bla CTX-M-15 are emergent, widely distributed, and predominant among ESBL-positive E. coli strains in the United States, among children and adults alike. Enhanced virulence and antimicrobial resistance have likely promoted the epidemiological success of these emerging public health threats.",
author = "Johnson, {James R.} and Carl Urban and Weissman, {Scott J.} and Jorgensen, {James H.} and Lewis, {James S.} and Glen Hansen and Edelstein, {Paul H.} and Ari Robicsek and Timothy Cleary and Javier Adachi and David Paterson and John Quinn and Hanson, {Nancy D.} and Johnston, {Brian D.} and Connie Clabots and Kuskowski, {Michael A.} and Bergsbaken, {Robert L.} and Hooton, {Thomas M.} and Michelle Hulse and Karen Lolans and Rob Owens and Elizabeth Palavecino and Karen Vigil",
year = "2012",
month = "5",
doi = "10.1128/AAC.05824-11",
language = "English",
volume = "56",
pages = "2364--2370",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Molecular epidemiological analysis of Escherichia coli sequence type ST131 (O25

T2 - H4) and bla CTX-M-15among extended-spectrum-β- lactamase-producing E. coli from the United States, 2000 to 2009

AU - Johnson, James R.

AU - Urban, Carl

AU - Weissman, Scott J.

AU - Jorgensen, James H.

AU - Lewis, James S.

AU - Hansen, Glen

AU - Edelstein, Paul H.

AU - Robicsek, Ari

AU - Cleary, Timothy

AU - Adachi, Javier

AU - Paterson, David

AU - Quinn, John

AU - Hanson, Nancy D.

AU - Johnston, Brian D.

AU - Clabots, Connie

AU - Kuskowski, Michael A.

AU - Bergsbaken, Robert L.

AU - Hooton, Thomas M.

AU - Hulse, Michelle

AU - Lolans, Karen

AU - Owens, Rob

AU - Palavecino, Elizabeth

AU - Vigil, Karen

PY - 2012/5

Y1 - 2012/5

N2 - Escherichia coli sequence type ST131 (from phylogenetic group B2), often carrying the extended-spectrum-β-lactamase (ESBL) gene bla CTX-M-15, is an emerging globally disseminated pathogen that has received comparatively little attention in the United States. Accordingly, a convenience sample of 351 ESBL-producing E. coli isolates from 15 U.S. centers (collected in 2000 to 2009) underwent PCR-based phylotyping and detection of ST131 and bla CTX-M-15. A total of 200 isolates, comprising 4 groups of 50 isolates each that were (i) bla CTX-M-15 negative non-ST131, (ii) bla CTX-M-15 positive non-ST131, (iii) bla CTX-M-15 negative ST131, or (iv) bla CTX-M-15 positive ST131, also underwent virulence genotyping, antimicrobial susceptibility testing, and pulsed-field gel electrophoresis (PFGE). Overall, 201 (57%) isolates exhibited bla CTX-M-15, whereas 165 (47%) were ST131. ST131 accounted for 56% of bla CTX-M-15-positive-versus 35% of bla CTX-M-15-negative isolates (PCTX-M-15 status, with groups A (bla CTX-M-15-positive isolates) andD(bla CTX-M-15- negative isolates) predominating. Both bla CTX-M-15 and ST131 occurred at all participating centers, were recovered from children and adults, increased significantly in prevalence post-2003, and were associated with molecularly inferred virulence. Compared with non- ST131 isolates, ST131 isolates had higher virulence scores, distinctive virulence profiles, and more-homogeneous PFGE profiles. bla CTX-M-15 was associated with extensive antimicrobial resistance and ST131 with fluoroquinolone resistance. Thus, E. coli ST131 and bla CTX-M-15 are emergent, widely distributed, and predominant among ESBL-positive E. coli strains in the United States, among children and adults alike. Enhanced virulence and antimicrobial resistance have likely promoted the epidemiological success of these emerging public health threats.

AB - Escherichia coli sequence type ST131 (from phylogenetic group B2), often carrying the extended-spectrum-β-lactamase (ESBL) gene bla CTX-M-15, is an emerging globally disseminated pathogen that has received comparatively little attention in the United States. Accordingly, a convenience sample of 351 ESBL-producing E. coli isolates from 15 U.S. centers (collected in 2000 to 2009) underwent PCR-based phylotyping and detection of ST131 and bla CTX-M-15. A total of 200 isolates, comprising 4 groups of 50 isolates each that were (i) bla CTX-M-15 negative non-ST131, (ii) bla CTX-M-15 positive non-ST131, (iii) bla CTX-M-15 negative ST131, or (iv) bla CTX-M-15 positive ST131, also underwent virulence genotyping, antimicrobial susceptibility testing, and pulsed-field gel electrophoresis (PFGE). Overall, 201 (57%) isolates exhibited bla CTX-M-15, whereas 165 (47%) were ST131. ST131 accounted for 56% of bla CTX-M-15-positive-versus 35% of bla CTX-M-15-negative isolates (PCTX-M-15 status, with groups A (bla CTX-M-15-positive isolates) andD(bla CTX-M-15- negative isolates) predominating. Both bla CTX-M-15 and ST131 occurred at all participating centers, were recovered from children and adults, increased significantly in prevalence post-2003, and were associated with molecularly inferred virulence. Compared with non- ST131 isolates, ST131 isolates had higher virulence scores, distinctive virulence profiles, and more-homogeneous PFGE profiles. bla CTX-M-15 was associated with extensive antimicrobial resistance and ST131 with fluoroquinolone resistance. Thus, E. coli ST131 and bla CTX-M-15 are emergent, widely distributed, and predominant among ESBL-positive E. coli strains in the United States, among children and adults alike. Enhanced virulence and antimicrobial resistance have likely promoted the epidemiological success of these emerging public health threats.

UR - http://www.scopus.com/inward/record.url?scp=84860204494&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860204494&partnerID=8YFLogxK

U2 - 10.1128/AAC.05824-11

DO - 10.1128/AAC.05824-11

M3 - Article

VL - 56

SP - 2364

EP - 2370

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 5

ER -