Molecular epidemiology of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from global clinical trials

Richard V. Goering, Ribhi M. Shawar, Nicole E. Scangarella, F. Patrick O'Hara, Heather Amrine-Madsen, Joshua M. West, Marybeth Dalessandro, Julie A. Becker, Stacey L. Walsh, Linda A. Miller, Stephanie F. Van Horn, Elizabeth S. Thomas, Monique E. Twynholm

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Abstract

Determining the genetic characteristics of Staphylococcus aureus is important for better understanding of the global and dynamic epidemiology of this organism as we witness the emergence and spread of virulent and antibiotic-resistant clones. We genotyped 292 S. aureus isolates (105 methicillin resistant and 187 methicillin susceptible) using a combination of pulsed-field gel electrophoresis, multilocus sequence typing, and SCCmec typing. In addition, S. aureus isolates were tested for the presence of the Panton-Valentine leukocidin (PVL) genes. Isolates were recovered from patients with uncomplicated skin infections in 10 different countries during five phase III global clinical trials of retapamulin, a new topical antibiotic agent. The most common methicillin-resistant clone had multilocus sequence type 8, pulsed-field type USA300, and SCCmec type IV and possessed the PVL genes. This clone was isolated exclusively in the United States. The most common PVL-positive, methicillin-susceptible clone had multilocus sequence type 121 and pulsed-field type USA1200. This clone was found primarily in South Africa and the Russian Federation. Other clones were found at lower frequencies and were limited in their geographic distribution. Overall, considerable genetic diversity was observed within multilocus sequence type clonal complexes and pulsed-field types.

Original languageEnglish
Pages (from-to)2842-2847
Number of pages6
JournalJournal of Clinical Microbiology
Volume46
Issue number9
DOIs
StatePublished - Sep 2008

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Methicillin Resistance
Methicillin
Molecular Epidemiology
Staphylococcus aureus
Clone Cells
Clinical Trials
Anti-Bacterial Agents
Multilocus Sequence Typing
Phase III Clinical Trials
Pulsed Field Gel Electrophoresis
South Africa
Genes
Epidemiology
Skin
Infection
Panton-Valentine leukocidin

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Molecular epidemiology of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from global clinical trials. / Goering, Richard V.; Shawar, Ribhi M.; Scangarella, Nicole E.; O'Hara, F. Patrick; Amrine-Madsen, Heather; West, Joshua M.; Dalessandro, Marybeth; Becker, Julie A.; Walsh, Stacey L.; Miller, Linda A.; Van Horn, Stephanie F.; Thomas, Elizabeth S.; Twynholm, Monique E.

In: Journal of Clinical Microbiology, Vol. 46, No. 9, 09.2008, p. 2842-2847.

Research output: Contribution to journalArticle

Goering, RV, Shawar, RM, Scangarella, NE, O'Hara, FP, Amrine-Madsen, H, West, JM, Dalessandro, M, Becker, JA, Walsh, SL, Miller, LA, Van Horn, SF, Thomas, ES & Twynholm, ME 2008, 'Molecular epidemiology of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from global clinical trials', Journal of Clinical Microbiology, vol. 46, no. 9, pp. 2842-2847. https://doi.org/10.1128/JCM.00521-08
Goering, Richard V. ; Shawar, Ribhi M. ; Scangarella, Nicole E. ; O'Hara, F. Patrick ; Amrine-Madsen, Heather ; West, Joshua M. ; Dalessandro, Marybeth ; Becker, Julie A. ; Walsh, Stacey L. ; Miller, Linda A. ; Van Horn, Stephanie F. ; Thomas, Elizabeth S. ; Twynholm, Monique E. / Molecular epidemiology of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from global clinical trials. In: Journal of Clinical Microbiology. 2008 ; Vol. 46, No. 9. pp. 2842-2847.
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