Abstract
A number of sibling species in the Simulium damnosum species complex transmit River Blindness (onchocerciasis) in tropical Africa. Black fly sibling species can be reliably recognized only through analysis of the larval polytene chromosomes. The routine, unambiguous assignment of the potentially disease-transmitting adult females to a particular species is virtually impossible. Two molecular assays to identify the sibling species of biting female flies are presented. The first relies on PCR-mediated amplification of internal transcribed spacers of the nuclear rDNA loci while the second involves immunoblotting of silk proteins which are expressed in both larvae and adult females. Both assays yield sibling-specific size markers that can be used to identify the sibling species of biting adult females. To illustrate the use of these assays, a molecular characterization is given for two S. damnosum siblings (squamosum, sirbanum) collected from cytologically verified populations, and a partial characterization of a third sibling (damnosum s. str.) found as a contaminant in these samples.
| Original language | English |
|---|---|
| Pages (from-to) | 628-634 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 194 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 30 1993 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Biophysics
- Biochemistry
Cite this
Molecular Identification of Onchocerciasis Vector Sibling Species in Black Flies (Diptera : Simuliidae). / Brockhouse, Charles; Vajime, C. G.; Marin, R.; Tanguay, R. M.
In: Biochemical and Biophysical Research Communications, Vol. 194, No. 2, 30.07.1993, p. 628-634.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular Identification of Onchocerciasis Vector Sibling Species in Black Flies (Diptera
T2 - Simuliidae)
AU - Brockhouse, Charles
AU - Vajime, C. G.
AU - Marin, R.
AU - Tanguay, R. M.
PY - 1993/7/30
Y1 - 1993/7/30
N2 - A number of sibling species in the Simulium damnosum species complex transmit River Blindness (onchocerciasis) in tropical Africa. Black fly sibling species can be reliably recognized only through analysis of the larval polytene chromosomes. The routine, unambiguous assignment of the potentially disease-transmitting adult females to a particular species is virtually impossible. Two molecular assays to identify the sibling species of biting female flies are presented. The first relies on PCR-mediated amplification of internal transcribed spacers of the nuclear rDNA loci while the second involves immunoblotting of silk proteins which are expressed in both larvae and adult females. Both assays yield sibling-specific size markers that can be used to identify the sibling species of biting adult females. To illustrate the use of these assays, a molecular characterization is given for two S. damnosum siblings (squamosum, sirbanum) collected from cytologically verified populations, and a partial characterization of a third sibling (damnosum s. str.) found as a contaminant in these samples.
AB - A number of sibling species in the Simulium damnosum species complex transmit River Blindness (onchocerciasis) in tropical Africa. Black fly sibling species can be reliably recognized only through analysis of the larval polytene chromosomes. The routine, unambiguous assignment of the potentially disease-transmitting adult females to a particular species is virtually impossible. Two molecular assays to identify the sibling species of biting female flies are presented. The first relies on PCR-mediated amplification of internal transcribed spacers of the nuclear rDNA loci while the second involves immunoblotting of silk proteins which are expressed in both larvae and adult females. Both assays yield sibling-specific size markers that can be used to identify the sibling species of biting adult females. To illustrate the use of these assays, a molecular characterization is given for two S. damnosum siblings (squamosum, sirbanum) collected from cytologically verified populations, and a partial characterization of a third sibling (damnosum s. str.) found as a contaminant in these samples.
UR - http://www.scopus.com/inward/record.url?scp=0027226139&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027226139&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1993.1867
DO - 10.1006/bbrc.1993.1867
M3 - Article
C2 - 8343149
AN - SCOPUS:0027226139
VL - 194
SP - 628
EP - 634
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -