Molecular relatedness of Staphylococcus epidermidis isolates obtained during a platelet transfusion-associated episode of sepsis

M. Shayegani, L. M. Parsons, A. L. Waring, J. Donhowe, Richard V. Goering, W. A. Archinal, J. Linden

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Abstract

Staphylococcus epidermidis was isolated from the blood of a 25-year-old pregnant woman following the administration of eight units of platelets. She had developed chills and a fever of 41.4°C soon after the transfusions were completed. S. epidermidis was also obtained from all eight platelet units, as well as from the packed-erythrocyte unit associated with the first unit of platelets. The isolation of the same organism from these epidemiologically related sources provided us with the opportunity to phenotypically and genetically characterize the isolates. Several typing methods, including four molecular techniques, were used to increase our chances of finding any differences between the isolates under investigation. Phenotypic analyses demonstrated that S. epidermidis isolates from the patient, platelet units, and erythrocyte unit reacted in exactly the same manner in 15 biochemical tests, exhibited slime production, and had the same antibiotic susceptibility pattern. Genetic analyses, which included plasmid profiles, plasmid cross-hybridization, field inversion gel electrophoresis, and ribotyping, substantiated the relationships between the S. epidermidis isolates from the patient, platelet units, and erythrocyte unit. Eight S. epidermidis control strains unrelated to the case were found to differ significantly from the platelet-related strain.

Original languageEnglish
Pages (from-to)2768-2773
Number of pages6
JournalJournal of Clinical Microbiology
Volume29
Issue number12
StatePublished - 1991
Externally publishedYes

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Platelet Transfusion
Staphylococcus epidermidis
Sepsis
Blood Platelets
Erythrocytes
Plasmids
Ribotyping
Chills
Pulsed Field Gel Electrophoresis
Pregnant Women
Fever
Anti-Bacterial Agents

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

Shayegani, M., Parsons, L. M., Waring, A. L., Donhowe, J., Goering, R. V., Archinal, W. A., & Linden, J. (1991). Molecular relatedness of Staphylococcus epidermidis isolates obtained during a platelet transfusion-associated episode of sepsis. Journal of Clinical Microbiology, 29(12), 2768-2773.

Molecular relatedness of Staphylococcus epidermidis isolates obtained during a platelet transfusion-associated episode of sepsis. / Shayegani, M.; Parsons, L. M.; Waring, A. L.; Donhowe, J.; Goering, Richard V.; Archinal, W. A.; Linden, J.

In: Journal of Clinical Microbiology, Vol. 29, No. 12, 1991, p. 2768-2773.

Research output: Contribution to journalArticle

Shayegani, M, Parsons, LM, Waring, AL, Donhowe, J, Goering, RV, Archinal, WA & Linden, J 1991, 'Molecular relatedness of Staphylococcus epidermidis isolates obtained during a platelet transfusion-associated episode of sepsis', Journal of Clinical Microbiology, vol. 29, no. 12, pp. 2768-2773.
Shayegani, M. ; Parsons, L. M. ; Waring, A. L. ; Donhowe, J. ; Goering, Richard V. ; Archinal, W. A. ; Linden, J. / Molecular relatedness of Staphylococcus epidermidis isolates obtained during a platelet transfusion-associated episode of sepsis. In: Journal of Clinical Microbiology. 1991 ; Vol. 29, No. 12. pp. 2768-2773.
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