TY - JOUR
T1 - Molecular typing of methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis
T2 - Comparison of results obtained in a multilaboratory effort using identical protocols and MRSA strains
AU - Multilaboratory Project Collaborators
AU - Chung, Marilyn
AU - De Lencastre, Hermínia
AU - Matthews, Peter
AU - Tomasz, Alexander
AU - Adamsson, Inger
AU - Aires de Sousa, Marta
AU - Camou, Teresa
AU - Cocuzza, Clementina
AU - Corso, Alejandra
AU - Couto, Isabel
AU - Dominguez, Angeles
AU - Gniadkowski, Marek
AU - Goering, Richard
AU - Gomes, Ana
AU - Kikuchi, Ken
AU - Marchese, Anna
AU - Mato, Rosario
AU - Melter, Oto
AU - Oliveira, Duarte
AU - Sá-Leão, Raquel
AU - Santos Sanches, Ilda
AU - Santos Sanches, Ilda
AU - Tassios, Panayotis T.
AU - Villari, Paolo
PY - 2009/1/28
Y1 - 2009/1/28
N2 - Pulsed-field gel electrophoresis (PFGE) has become the gold standard of molecular methods in epidemiological investigations. In spite of its high resolving power, use of the method has been hampered by inadequate laboratory-to-laboratory reproducibility. In the project described here we have addressed this problem by organizing a multilaboratory effort in which the same bacterial strains (subtype variants of the Iberian and Brazilian methicillin-resistant Staphylococcus aureus-MRSA-clones) were analyzed by twenty Investigators in thirteen different laboratories according to an indentical protocol, which is reproduced here in detail. PFGE patterns obtained were analyzed at a central laboratory in order to identify specific technical problems that produced substandard macrorestriction patterns. The results including the specific technical problems and their most likely causes are described in this communication. Also listed are seven major epidemic clones of MRSA which have been characterized by molecular fingerprinting techniques and the prototypes of which have been deposited at the American Type Culture Collection, from where they will be available for interested investigators for the purpose of typing MRSA isolates. It is hoped that this communication will contribute to the improvement of the reproducibility and technical/aesthetic quality of PFGE analysis.
AB - Pulsed-field gel electrophoresis (PFGE) has become the gold standard of molecular methods in epidemiological investigations. In spite of its high resolving power, use of the method has been hampered by inadequate laboratory-to-laboratory reproducibility. In the project described here we have addressed this problem by organizing a multilaboratory effort in which the same bacterial strains (subtype variants of the Iberian and Brazilian methicillin-resistant Staphylococcus aureus-MRSA-clones) were analyzed by twenty Investigators in thirteen different laboratories according to an indentical protocol, which is reproduced here in detail. PFGE patterns obtained were analyzed at a central laboratory in order to identify specific technical problems that produced substandard macrorestriction patterns. The results including the specific technical problems and their most likely causes are described in this communication. Also listed are seven major epidemic clones of MRSA which have been characterized by molecular fingerprinting techniques and the prototypes of which have been deposited at the American Type Culture Collection, from where they will be available for interested investigators for the purpose of typing MRSA isolates. It is hoped that this communication will contribute to the improvement of the reproducibility and technical/aesthetic quality of PFGE analysis.
UR - http://www.scopus.com/inward/record.url?scp=0033664479&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033664479&partnerID=8YFLogxK
U2 - 10.1089/mdr.2000.6.189
DO - 10.1089/mdr.2000.6.189
M3 - Article
C2 - 11144419
AN - SCOPUS:0033664479
VL - 6
SP - 189
EP - 198
JO - Microbial Drug Resistance
JF - Microbial Drug Resistance
SN - 1076-6294
IS - 3
ER -