Multiple pathogenic factor-induced complications of cirrhosis in rats

A new model of hepatopulmonary syndrome with intestinal endotoxemia

Hui Ying Zhang, De Wu Han, Zhong Fu Zhao, Ming She Liu, Yan Ju Wu, Xian-Ming Chen, Cheng Ji

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Aim: To develop and characterize a practical model of Hepatopulmonary syndrome (HPS) in rats. Methods: The experimental animals were randomized into five feeding groups: (1) control (fed standard diet), (2) control plus intraperitoneal injection with lipopolysaccharide (LPS), (3) cirrhosis (fed a diet of maize flour, lard, cholesterol, and alcohol plus subcutaneously injection with carbon tetrachloride (CCI4) oil solution), (4) cirrhosis plus LPS, and (5) cirrhosis plus glycine and LPS. The blood, liver and lung tissues of rats were sampled for analysis and characterization. Technetium 99m-labeled macroaggregated albumin (Tc99m-MAA) was used to test the dilatation of pulmonary microvasculature. Results: Typical cirrhosis and subsequent hepatopulmonary syndrome was observed in the cirrhosis groups after an 8 wk feeding period. In rats with cirrhosis, there were a decreased PaO2 and PaCO2 in arterial blood, markedly decreased arterial O2 content, a significantly increased alveolar to arterial oxygen gradient, an increased number of bacterial translocated within mesenteric lymph node, a significant higher level of LPS and tumor necrosis factor-α (TNF-α) in plasma, and a significant greater ratio of Tc99m-MAA brain-over-lung radioactivity. After LPS administration in rats with cirrhosis, various pathological parameters got worse and pulmonary edema formed. The predisposition of glycine antagonized the effects of LPS and significantly alleviated various pathological alterations. Conclusion: The results suggest that: (1) a characteristic rat model of HPS can be non-invasively induced by multiple pathogenic factors including high fat diet, alcohol, cholesterol and CCI4; (2) this model can be used for study of hepatopulmonary syndrome and is clinically relevant; and (3) intestinal endotoxemia (IETM) and its accompanying cytokines, such as TNF-α, exert a crucial role in the pathogenesis of HPS in this model.

Original languageEnglish
Pages (from-to)3500-3507
Number of pages8
JournalWorld Journal of Gastroenterology
Volume13
Issue number25
StatePublished - Jul 7 2007

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Hepatopulmonary Syndrome
Endotoxemia
Fibrosis
Lipopolysaccharides
Technetium
Lung
Glycine Agents
Albumins
Tumor Necrosis Factor-alpha
Cholesterol
Alcohols
Diet
Carbon Tetrachloride
High Fat Diet
Flour
Pulmonary Edema
Microvessels
Intraperitoneal Injections
Glycine
Radioactivity

All Science Journal Classification (ASJC) codes

  • Gastroenterology

Cite this

Multiple pathogenic factor-induced complications of cirrhosis in rats : A new model of hepatopulmonary syndrome with intestinal endotoxemia. / Zhang, Hui Ying; Han, De Wu; Zhao, Zhong Fu; Liu, Ming She; Wu, Yan Ju; Chen, Xian-Ming; Ji, Cheng.

In: World Journal of Gastroenterology, Vol. 13, No. 25, 07.07.2007, p. 3500-3507.

Research output: Contribution to journalArticle

Zhang, Hui Ying ; Han, De Wu ; Zhao, Zhong Fu ; Liu, Ming She ; Wu, Yan Ju ; Chen, Xian-Ming ; Ji, Cheng. / Multiple pathogenic factor-induced complications of cirrhosis in rats : A new model of hepatopulmonary syndrome with intestinal endotoxemia. In: World Journal of Gastroenterology. 2007 ; Vol. 13, No. 25. pp. 3500-3507.
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abstract = "Aim: To develop and characterize a practical model of Hepatopulmonary syndrome (HPS) in rats. Methods: The experimental animals were randomized into five feeding groups: (1) control (fed standard diet), (2) control plus intraperitoneal injection with lipopolysaccharide (LPS), (3) cirrhosis (fed a diet of maize flour, lard, cholesterol, and alcohol plus subcutaneously injection with carbon tetrachloride (CCI4) oil solution), (4) cirrhosis plus LPS, and (5) cirrhosis plus glycine and LPS. The blood, liver and lung tissues of rats were sampled for analysis and characterization. Technetium 99m-labeled macroaggregated albumin (Tc99m-MAA) was used to test the dilatation of pulmonary microvasculature. Results: Typical cirrhosis and subsequent hepatopulmonary syndrome was observed in the cirrhosis groups after an 8 wk feeding period. In rats with cirrhosis, there were a decreased PaO2 and PaCO2 in arterial blood, markedly decreased arterial O2 content, a significantly increased alveolar to arterial oxygen gradient, an increased number of bacterial translocated within mesenteric lymph node, a significant higher level of LPS and tumor necrosis factor-α (TNF-α) in plasma, and a significant greater ratio of Tc99m-MAA brain-over-lung radioactivity. After LPS administration in rats with cirrhosis, various pathological parameters got worse and pulmonary edema formed. The predisposition of glycine antagonized the effects of LPS and significantly alleviated various pathological alterations. Conclusion: The results suggest that: (1) a characteristic rat model of HPS can be non-invasively induced by multiple pathogenic factors including high fat diet, alcohol, cholesterol and CCI4; (2) this model can be used for study of hepatopulmonary syndrome and is clinically relevant; and (3) intestinal endotoxemia (IETM) and its accompanying cytokines, such as TNF-α, exert a crucial role in the pathogenesis of HPS in this model.",
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