Multiplex high-resolution melting analysis as a diagnostic tool for detection of plasmid-mediated AmpC β-lactamase genes

Chelsie N. Geyer, Nancy D. Hanson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

High-resolution melting (HRM) analysis can be a diagnostic tool to evaluate the presence of resistance genes with the added bonus of discriminating sequence modifications. A real-time, multiplex PCR assay using HRM was designed for the detection of plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 96% and 100%, respectively.

Original languageEnglish
Pages (from-to)1262-1265
Number of pages4
JournalJournal of Clinical Microbiology
Volume52
Issue number4
DOIs
StatePublished - 2014

Fingerprint

Freezing
Plasmids
Multiplex Polymerase Chain Reaction
Genes
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Medicine(all)

Cite this

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abstract = "High-resolution melting (HRM) analysis can be a diagnostic tool to evaluate the presence of resistance genes with the added bonus of discriminating sequence modifications. A real-time, multiplex PCR assay using HRM was designed for the detection of plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 96{\%} and 100{\%}, respectively.",
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