Mycobacterial antigens attenuate late phase response, airway hyperresponsiveness, and bronchoalveolar lavage eosinophilia in a mouse model of bronchial asthma

Michael T. Hopfenspirger, Sharidan K. Parr, Russell J. Hopp, Robert G. Townley, Devendra K. Agrawal

Research output: Contribution to journalArticle

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Abstract

Allergens, in combination with genetic predisposition, drive undifferentiated T cells towards the type 2 T cells. Some childhood infections may activate the production of a type 1 T cell profile. It is reasonable to speculate that a decrease in childhood infections may increase the incidence of allergy by allowing the immune balance to shift towards the type 2 T cells. We hypothesized that pre-exposure of mycobacterial antigens in sensitized mice would prevent the development of asthma-like conditions. Specifically, we examined the effect of mycobacterial antigens, Bacillus Calmette-Guérin (BCG) vaccine and Mycobacterium vaccae, on antigen-induced bronchoconstriction, airway hyperresponsiveness to methacholine, bronchoalveolar lavage eosinophilia, and plasma IL-4 and IL-12 levels in ovalbumin (OVA)-sensitized and challenged Balb/c mice. Challenge with OVA produced a 2-3-fold increase in bronchoconstriction within 3-5 min, followed by a delayed response after 60 min, the latter of which was significantly attenuated by both BCG and M. vaccae. Airway hyperresponsiveness to methacholine 24 h after OVA challenge was prevented by BCG and M. vaccae. Airway eosinophilia was also prevented by BCG and M. vaccae. The plasma IL-12 levels were significantly increased and plasma IL-4 levels were significantly decreased following BCG or M. vaccae administration in OVA-sensitized and challenged mice. Interestingly, a significant increase in plasma IL-12 was observed with BCG as compared to M. vaccae administration, suggesting a stronger type 1 response to BCG. These data support our hypothesis and suggest that BCG and M. vaccae may prevent the underlying pathophysiological changes in asthma.

Original languageEnglish
Pages (from-to)1743-1751
Number of pages9
JournalInternational Immunopharmacology
Volume1
Issue number9-10
DOIs
StatePublished - 2001

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Eosinophilia
Bronchoalveolar Lavage
Bacillus
Asthma
Antigens
Ovalbumin
Interleukin-12
T-Lymphocytes
Bronchoconstriction
Methacholine Chloride
Interleukin-4
BCG Vaccine
Genetic Predisposition to Disease
Mycobacterium
Infection
Allergens
Hypersensitivity
Incidence

All Science Journal Classification (ASJC) codes

  • Immunology
  • Pharmacology

Cite this

Mycobacterial antigens attenuate late phase response, airway hyperresponsiveness, and bronchoalveolar lavage eosinophilia in a mouse model of bronchial asthma. / Hopfenspirger, Michael T.; Parr, Sharidan K.; Hopp, Russell J.; Townley, Robert G.; Agrawal, Devendra K.

In: International Immunopharmacology, Vol. 1, No. 9-10, 2001, p. 1743-1751.

Research output: Contribution to journalArticle

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