Nasal associated lymphoid tissue of the Syrian golden hamster expresses high levels of PrPC

Melissa D. Clouse, Ronald A. Shikiya, Jason C. Bartz, Anthony Kincaid

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The key event in the pathogenesis of the transmissible spongiform encephalopathies is a template-dependent misfolding event where an infectious isoform of the prion protein (PrPSc) comes into contact with native prion protein (PrPC) and changes its conformation to PrPSc. In many extraneurally inoculated models of prion disease this PrPC misfolding event occurs in lymphoid tissues prior to neuroinvasion. The primary objective of this study was to compare levels of total PrPC in hamster lymphoid tissues involved in the early pathogenesis of prion disease. Lymphoid tissues were collected from golden Syrian hamsters and Western blot analysis was performed to quantify PrPC levels. PrPC immunohistochemistry (IHC) of paraffin embedded tissue sections was performed to identify PrPC distribution in tissues of the lymphoreticular system. Nasal associated lymphoid tissue contained the highest amount of total PrPC followed by Peyer 's patches, mesenteric and submandibular lymph nodes, and spleen. The relative levels of PrPC expression in IHC processed tissue correlated strongly with the Western blot data, with high levels of PrPC corresponding with a higher percentage of PrPC positive B cell follicles. High levels of PrPC in lymphoid tissues closely associated with the nasal cavity could contribute to the relative increased efficiency of the nasal route of entry of prions, compared to other routes of infection.

Original languageEnglish
Article numbere0117935
JournalPLoS One
Volume10
Issue number2
DOIs
StatePublished - Feb 2 2015

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Mesocricetus
Lymphoid Tissue
Nose
Prion Diseases
Tissue
prion diseases
prions
Prions
PrPSc proteins
Western Blotting
Immunohistochemistry
immunohistochemistry
Peyer's Patches
Western blotting
Nasal Cavity
pathogenesis
PrPC Proteins
Tissue Distribution
Cricetinae
Paraffin

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Nasal associated lymphoid tissue of the Syrian golden hamster expresses high levels of PrPC . / Clouse, Melissa D.; Shikiya, Ronald A.; Bartz, Jason C.; Kincaid, Anthony.

In: PLoS One, Vol. 10, No. 2, e0117935, 02.02.2015.

Research output: Contribution to journalArticle

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