NF45 and NF90 bind HIV-1 RNA and modulate HIV gene expression

Research output: Contribution to journalArticle

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Abstract

A previous proteomic screen in our laboratory identified nuclear factor 45 (NF45) and nuclear factor 90 (NF90) as potential cellular factors involved in human immunodeficiency virus type 1 (HIV-1) replication. Both are RNA binding proteins that regulate gene expression; and NF90 has been shown to regulate the expression of cyclin T1 which is required for Tat-dependent trans-activation of viral gene expression. In this study the roles of NF45 and NF90 in HIV replication were investigated through overexpression studies. Ectopic expression of either factor potentiated HIV infection, gene expression, and virus production. Deletion of the RNA binding domains of NF45 and NF90 diminished the enhancement of HIV infection and gene expression. Both proteins were found to interact with the HIV RNA. RNA decay assays demonstrated that NF90, but not NF45, increased the half-life of the HIV RNA. Overall, these studies indicate that both NF45 and NF90 potentiate HIV infection through their RNA binding domains.

Original languageEnglish
Article number23
JournalViruses
Volume8
Issue number2
DOIs
StatePublished - Feb 16 2016

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Nuclear Factor 90 Proteins
HIV-1
HIV
RNA
Gene Expression
HIV Infections
Cyclin T
RNA-Binding Proteins
Viral Genes
RNA Stability
Virus Replication
Proteomics
Half-Life
Viruses

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Virology

Cite this

NF45 and NF90 bind HIV-1 RNA and modulate HIV gene expression. / Li, Yan; Belshan, Michael A.

In: Viruses, Vol. 8, No. 2, 23, 16.02.2016.

Research output: Contribution to journalArticle

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