TY - JOUR
T1 - Nitric oxide concentrations and cerebrospinal fluid parameters in an experimental animal model of Streptococcus pneumoniae meningitis
AU - Destache, Christopher J.
AU - Pakiz, Catherine B.
AU - Dash, Alekha K.
AU - Larsen, Christine
PY - 1998/5/1
Y1 - 1998/5/1
N2 - Streptococcus pneumoniae is a common cause of meningitis. Nitric oxide (NO) has been implicated in causing cerebral edema. Modulating NO production in cerebrospinal fluid (CSF) may have a role in the treatment of bacterial meningitis. Experimental S. pneumoniae meningitis was induced in a rabbit model to determine CSF parameters and NO concentrations. An electrochemical probe in the CSF throughout the 7-hour experiment monitored NO concentrations. The animals had S. pneumoniae (10
5) injected intracisternally and incubated for 1 hour. Cerebrospinal fluid 200-300 μl was obtained by intracisternal puncture at zero, 2, 4, and 7 hours after drug administration to measure glucose, protein, and lactic acid by standard chemical methods. White blood cell count was measured by hemocytometry. Three groups of five animals were used - control (C), ceftriaxone (CTX), and ceftriaxone plus dexamethasone (CTX+D). Ceftriaxone concentrations in CSF were obtained by microdialysis and analyzed by high-performance liquid chromatography. Mean (± SEM) CSF white blood cell count was significantly higher at 2 hours in the C group than in the other two groups (C 7307 ± 1302, CTX 605 ± 345, CTX+D 730 ± 43/mm
3, p0.05), whereas they did not rise significantly until 7 hours in the CTX group (CTX 18.7 ± 0.7, C 8.9 ± 0.4 μM, p=0.055; CTX+D 8.1 ± 2.2 μM, p
AB - Streptococcus pneumoniae is a common cause of meningitis. Nitric oxide (NO) has been implicated in causing cerebral edema. Modulating NO production in cerebrospinal fluid (CSF) may have a role in the treatment of bacterial meningitis. Experimental S. pneumoniae meningitis was induced in a rabbit model to determine CSF parameters and NO concentrations. An electrochemical probe in the CSF throughout the 7-hour experiment monitored NO concentrations. The animals had S. pneumoniae (10
5) injected intracisternally and incubated for 1 hour. Cerebrospinal fluid 200-300 μl was obtained by intracisternal puncture at zero, 2, 4, and 7 hours after drug administration to measure glucose, protein, and lactic acid by standard chemical methods. White blood cell count was measured by hemocytometry. Three groups of five animals were used - control (C), ceftriaxone (CTX), and ceftriaxone plus dexamethasone (CTX+D). Ceftriaxone concentrations in CSF were obtained by microdialysis and analyzed by high-performance liquid chromatography. Mean (± SEM) CSF white blood cell count was significantly higher at 2 hours in the C group than in the other two groups (C 7307 ± 1302, CTX 605 ± 345, CTX+D 730 ± 43/mm
3, p0.05), whereas they did not rise significantly until 7 hours in the CTX group (CTX 18.7 ± 0.7, C 8.9 ± 0.4 μM, p=0.055; CTX+D 8.1 ± 2.2 μM, p
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M3 - Article
C2 - 9620112
AN - SCOPUS:0031981875
VL - 18
SP - 612
EP - 619
JO - Pharmacotherapy
JF - Pharmacotherapy
SN - 0277-0008
IS - 3
ER -