Notes: In vitro generation of high-titer prions

Ronald A. Shikiya, Jason C. Bartz

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Prions are composed mainly, if not entirely, of PrP Sc, an infectious misfolded isoform of PrP C, the normal isoform of the prion protein. Here we show that protein misfolding cyclic amplification (PMCA)-generated hypertransmissible mink encephalopathy (HY TME) PrP Sc is highly infectious and has a titer that is similar, if not identical, to that associated with brain tissue from animals infected with the HY TME agent that are in the terminal stage of disease. These data demonstrate that PMCA efficiently replicates the prion agent and provide further support for the hypothesis that in vitro-generated prions are bona fide and are not due to contamination.

Original languageEnglish (US)
Pages (from-to)13439-13442
Number of pages4
JournalJournal of Virology
Issue number24
StatePublished - Dec 2011

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology


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