Novel aquatic silk genes from Simulium (Psilozia) vittatum (Zett) Diptera

Simuliidae

Alexie Papanicolaou, Angelica Woo, Brianna Brei, Danjun Ma, Andrius Masedunskas, Elmer Gray, Gary Guishan Xiao, Soochin Cho, Charles Brockhouse

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The silks of arthropods have an elementary role in the natural history of the organisms that spin them, yet they are coded by rapidly evolving genes leading some authors to speculate that silk proteins are non-homologous proteins co-opted multiple times independently for similar functions. However, some general structural patterns are emerging. In this work we identified three major silk gland proteins using a combined biochemical, proteomic, next-generation sequencing and bioinformatic approach. Biochemical characterization determined that they were phosphorylated with multiple isoforms and potentially differential phosphorylation. Structural characterization showed that their structure was more similar to silk proteins from distantly related aquatic Trichopteran species than more closely related terrestrial or aquatic Diptera. Overall, our approach is easily transferable to any non-model species and if used across a larger number of aquatic species, we will be able to better understand the processes involved in linking the secondary structure of silk proteins with their function between in an organisms and its habitat.

Original languageEnglish
Pages (from-to)1181-1188
Number of pages8
JournalInsect Biochemistry and Molecular Biology
Volume43
Issue number12
DOIs
StatePublished - Dec 2013

Fingerprint

Simulium vittatum
silk proteins
Simuliidae
Silk
silk
Diptera
Genes
silk glands
Proteins
genes
organisms
natural history
bioinformatics
proteomics
arthropods
phosphorylation
Secondary Protein Structure
proteins
Phosphorylation
Arthropods

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Insect Science

Cite this

Novel aquatic silk genes from Simulium (Psilozia) vittatum (Zett) Diptera : Simuliidae. / Papanicolaou, Alexie; Woo, Angelica; Brei, Brianna; Ma, Danjun; Masedunskas, Andrius; Gray, Elmer; Xiao, Gary Guishan; Cho, Soochin; Brockhouse, Charles.

In: Insect Biochemistry and Molecular Biology, Vol. 43, No. 12, 12.2013, p. 1181-1188.

Research output: Contribution to journalArticle

Papanicolaou, Alexie ; Woo, Angelica ; Brei, Brianna ; Ma, Danjun ; Masedunskas, Andrius ; Gray, Elmer ; Xiao, Gary Guishan ; Cho, Soochin ; Brockhouse, Charles. / Novel aquatic silk genes from Simulium (Psilozia) vittatum (Zett) Diptera : Simuliidae. In: Insect Biochemistry and Molecular Biology. 2013 ; Vol. 43, No. 12. pp. 1181-1188.
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