Novel mutation in the KCNQ4 gene in a large kindred with dominant progressive hearing loss

Zohreh Talebizadeh, Philip M. Kelley, James W. Askew, Kirk Beisel, Shelley D. Smith

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Analysis of genotyping of a five-generation American family with nonsyndromic dominant progressive hearing loss indicated linkage to the DFNA2 locus on chromosome 1p34. This kindred consists of 170 individuals, of which 51 are affected. Pure tone audiograms, medical records, and blood samples were obtained from 36 family members. Linkage analysis with five microsatellite markers spanning the region around DFNA2 produced a lod score of 6.6 for the marker MYCL1 at θ = 0.0. Hearing loss in this family showed a very similar pattern as the first reported American family with the same linkage. High frequency hearing loss was detectable as early as 3 years of age, and progressed to severe to profound loss by the fourth decade. Using intronic primers, we screened the coding region of the KCNQ4 gene. Heteroduplex analysis followed by direct sequencing identified a T→C transition at position 842, which would produce an L281S amino acid substitution. The observed mutation was shown to segregate completely with affected status in this family. The L281 residue is significantly conserved among the other members of the voltage-gated K+ channel genes superfamily. Hydrophobicity analysis indicated that L281S substitution would lower formation of the β structure at the P region of this ion channel. Mutation analysis of KCNQ4 was also performed on 80 unrelated probands from families with recessive or dominant nonsyndromic hearing loss. None of these cases showed a truncated mutation in KCNQ4.

Original languageEnglish
Pages (from-to)493-501
Number of pages9
JournalHuman Mutation
Volume14
Issue number6
DOIs
StatePublished - 1999

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Hearing Loss
Mutation
Genes
High-Frequency Hearing Loss
Heteroduplex Analysis
Lod Score
Voltage-Gated Potassium Channels
Amino Acid Substitution
Hydrophobic and Hydrophilic Interactions
Ion Channels
Microsatellite Repeats
Medical Records
Chromosomes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

Novel mutation in the KCNQ4 gene in a large kindred with dominant progressive hearing loss. / Talebizadeh, Zohreh; Kelley, Philip M.; Askew, James W.; Beisel, Kirk; Smith, Shelley D.

In: Human Mutation, Vol. 14, No. 6, 1999, p. 493-501.

Research output: Contribution to journalArticle

Talebizadeh, Zohreh ; Kelley, Philip M. ; Askew, James W. ; Beisel, Kirk ; Smith, Shelley D. / Novel mutation in the KCNQ4 gene in a large kindred with dominant progressive hearing loss. In: Human Mutation. 1999 ; Vol. 14, No. 6. pp. 493-501.
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