Nuclear delivery of parasite Cdg2_FLc_0220 RNA transcript to epithelial cells during Cryptosporidium parvum infection modulates host gene transcription

Guang Hui Zhao, Ai Yu Gong, Yang Wang, Xin Tian Zhang, Min Li, Nicholas W. Mathy, Xian-Ming Chen

Research output: Contribution to journalArticle

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Abstract

Intestinal infection by the zoonotic protozoan, Cryptosporidium parvum, causes significant alterations in the gene expression profile in host epithelial cells. The molecular mechanisms of how C. parvum may modulate host cell gene transcription and the pathological significance of such alterations are largely unclear. Previous studies demonstrate that a panel of parasite RNA transcripts are delivered into infected host cells and may modulate host gene transcription. Using in vitro models of intestinal cryptosporidiosis, in this study, we analyzed the impact of host delivery of C. parvum Cdg2_FLc_0220 RNA transcript on host gene expression profile. We found that alterations in host gene expression profile following C. parvum infection were partially associated with the nuclear delivery of Cdg2_FLc_0220. Specifically, we identified a total of 46 overlapping upregulated genes and 8 overlapping downregulated genes in infected cells and cells transfected with Full-Cdg2_FLc_0220. Trans-suppression of the DAZ interacting zinc finger protein 1 like (DZIP1L) gene, the top overlapping downregulated gene in host cells following C. parvum infection and cells transfected with Full-Cdg2_FLc_0220, was mediated by G9a, independent of PRDM1. Cdg2_FLc_0220-mediated trans-suppression of the DZIP1L gene was independent of H3K9 and H3K27 methylation. Data from this study provide additional evidence that delivery of C. parvum Cdg2_FLc_0220 RNA transcript in infected epithelial cells modulates the transcription of host genes, contributing to the alterations in the gene expression profile in host epithelial cells during C. parvum infection.

Original languageEnglish (US)
Pages (from-to)27-33
Number of pages7
JournalVeterinary Parasitology
Volume251
DOIs
StatePublished - Feb 15 2018

Fingerprint

Cryptosporidium parvum
messenger RNA
Parasites
epithelial cells
transcription (genetics)
Epithelial Cells
RNA
parasites
Overlapping Genes
Transcriptome
Infection
infection
Genes
genes
Zinc Fingers
gene expression
zinc finger motif
cells
Down-Regulation
Protozoan Infections

All Science Journal Classification (ASJC) codes

  • Parasitology
  • veterinary(all)

Cite this

Nuclear delivery of parasite Cdg2_FLc_0220 RNA transcript to epithelial cells during Cryptosporidium parvum infection modulates host gene transcription. / Zhao, Guang Hui; Gong, Ai Yu; Wang, Yang; Zhang, Xin Tian; Li, Min; Mathy, Nicholas W.; Chen, Xian-Ming.

In: Veterinary Parasitology, Vol. 251, 15.02.2018, p. 27-33.

Research output: Contribution to journalArticle

Zhao, Guang Hui ; Gong, Ai Yu ; Wang, Yang ; Zhang, Xin Tian ; Li, Min ; Mathy, Nicholas W. ; Chen, Xian-Ming. / Nuclear delivery of parasite Cdg2_FLc_0220 RNA transcript to epithelial cells during Cryptosporidium parvum infection modulates host gene transcription. In: Veterinary Parasitology. 2018 ; Vol. 251. pp. 27-33.
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abstract = "Intestinal infection by the zoonotic protozoan, Cryptosporidium parvum, causes significant alterations in the gene expression profile in host epithelial cells. The molecular mechanisms of how C. parvum may modulate host cell gene transcription and the pathological significance of such alterations are largely unclear. Previous studies demonstrate that a panel of parasite RNA transcripts are delivered into infected host cells and may modulate host gene transcription. Using in vitro models of intestinal cryptosporidiosis, in this study, we analyzed the impact of host delivery of C. parvum Cdg2_FLc_0220 RNA transcript on host gene expression profile. We found that alterations in host gene expression profile following C. parvum infection were partially associated with the nuclear delivery of Cdg2_FLc_0220. Specifically, we identified a total of 46 overlapping upregulated genes and 8 overlapping downregulated genes in infected cells and cells transfected with Full-Cdg2_FLc_0220. Trans-suppression of the DAZ interacting zinc finger protein 1 like (DZIP1L) gene, the top overlapping downregulated gene in host cells following C. parvum infection and cells transfected with Full-Cdg2_FLc_0220, was mediated by G9a, independent of PRDM1. Cdg2_FLc_0220-mediated trans-suppression of the DZIP1L gene was independent of H3K9 and H3K27 methylation. Data from this study provide additional evidence that delivery of C. parvum Cdg2_FLc_0220 RNA transcript in infected epithelial cells modulates the transcription of host genes, contributing to the alterations in the gene expression profile in host epithelial cells during C. parvum infection.",
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AU - Mathy, Nicholas W.

AU - Chen, Xian-Ming

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