Nucleolus size varies with sex, ploidy and gene dosage in insects

Eugenia Elisabeth Montiel, Maria Inmaculada Manrique-Poyato, Sonia Rocha-Sanchez, Maria Dolores López-León, Josefa Cabrero, Francisco Perfectti, Juan Pedro M Camacho

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The nucleolus constitutes a cytologically visible phenotype for ribosomal DNA (rDNA). Nucleolar size, as determined by silver staining, is a good indicator of cell proliferation rate and biosynthetic activity. Nevertheless, the relationship between rDNA content and sexual dimorphism for nucleolar size is not well documented. In the present study, the impact of sex and ploidy level on nucleolar size is investigated in three haplo/diploid and three diplo/diploid species of insect. Nucleolar sizes are found to be proportional to ploidy level in the haplo/diploid hymenopterans Trypoxylon albitarse and Nasonia vitripennis. Conversely, in the ant Messor barbarus, nucleolar sizes are larger in haploid males (winged) than diploid females (apterous). Among the diplo/diploid species, evidence for gene dosage compensation on nucleolar activity is suggested by the absence of sex differences in Drosophila simulans, a species in which rDNA is limited to the X chromosome. By contrast, in the grasshopper Stenobothrus festivus, another species with rRNA genes restricted to the X chromosome, the size of the nucleolus is significantly larger in females than in males. Additionally, in the grasshopper Chorthippus parallelus, where rDNA is distributed evenly on several autosomes of males and females, the females also show larger nucleoli than males. In both grasshopper species, the magnitude of the female/male ratio for nucleolus area is very similar to the body size ratio, suggesting that body size, as well as sex, ploidy, gene dosage and physiological activity, may be an important determinant of nucleolus area.

Original languageEnglish
Pages (from-to)145-152
Number of pages8
JournalPhysiological Entomology
Volume37
Issue number2
DOIs
StatePublished - Jun 2012

Fingerprint

gene dosage
Gene Dosage
ploidy
Ploidies
cell nucleolus
Diploidy
Insects
Ribosomal DNA
diploidy
ribosomal DNA
Grasshoppers
insect
grasshoppers
insects
gene
gender
grasshopper
DNA
Body Size
X Chromosome

All Science Journal Classification (ASJC) codes

  • Insect Science
  • Physiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Montiel, E. E., Manrique-Poyato, M. I., Rocha-Sanchez, S., López-León, M. D., Cabrero, J., Perfectti, F., & Camacho, J. P. M. (2012). Nucleolus size varies with sex, ploidy and gene dosage in insects. Physiological Entomology, 37(2), 145-152. https://doi.org/10.1111/j.1365-3032.2011.00822.x

Nucleolus size varies with sex, ploidy and gene dosage in insects. / Montiel, Eugenia Elisabeth; Manrique-Poyato, Maria Inmaculada; Rocha-Sanchez, Sonia; López-León, Maria Dolores; Cabrero, Josefa; Perfectti, Francisco; Camacho, Juan Pedro M.

In: Physiological Entomology, Vol. 37, No. 2, 06.2012, p. 145-152.

Research output: Contribution to journalArticle

Montiel, EE, Manrique-Poyato, MI, Rocha-Sanchez, S, López-León, MD, Cabrero, J, Perfectti, F & Camacho, JPM 2012, 'Nucleolus size varies with sex, ploidy and gene dosage in insects', Physiological Entomology, vol. 37, no. 2, pp. 145-152. https://doi.org/10.1111/j.1365-3032.2011.00822.x
Montiel EE, Manrique-Poyato MI, Rocha-Sanchez S, López-León MD, Cabrero J, Perfectti F et al. Nucleolus size varies with sex, ploidy and gene dosage in insects. Physiological Entomology. 2012 Jun;37(2):145-152. https://doi.org/10.1111/j.1365-3032.2011.00822.x
Montiel, Eugenia Elisabeth ; Manrique-Poyato, Maria Inmaculada ; Rocha-Sanchez, Sonia ; López-León, Maria Dolores ; Cabrero, Josefa ; Perfectti, Francisco ; Camacho, Juan Pedro M. / Nucleolus size varies with sex, ploidy and gene dosage in insects. In: Physiological Entomology. 2012 ; Vol. 37, No. 2. pp. 145-152.
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