Odontogenic tumours in the v-Ha-ras (TG · AC) transgenic mouse

J. T. Wright, Laura A. Hansen, J. Mahler, C. Szczesniak, J. W. Spalding

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A line of homozygous transgenic mice (TG·AC) carrying a v-Ha-ras gene fused to the promoter of the zeta globin gene produces a variety of mesenchymal and epithelial neoplasms including odontogenic tumours. The 1-year incidence of odontogenic tumour formation in these mice was approx. 35%. Tumours formed more often in the mandible than maxilla. The various types of tumours frequently presented with: (1) primarily mesenchymal cells in a dense fibrous-like matrix, or (2) loose stroma surrounded by anastomosing cords of epithelial cells that exhibited squamous differentiation, or (3) odontomas forming mineralized tooth structures by well-differentiated odontoblasts and ameloblasts. Some tumours had areas with all three of these characteristics. Mineralized dentine and enamel in the odontomas were morphologically similar to those of normal murine teeth. Odontogenic tumours expressed the v-Ha-ras transgene that was primarily localized to the mesenchymal cells. Proliferating-cell nuclear antigen immunohistochemistry showed that the mesenchymal cells adjacent to the epithelial cords not only expressed the ras transgene but were also actively proliferating. The TG·AC mouse provides an excellent model for the study of odontogenic tumours and tooth development.

Original languageEnglish
Pages (from-to)631-638
Number of pages8
JournalArchives of Oral Biology
Volume40
Issue number7
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Odontogenic Tumors
Transgenic Mice
Odontoma
Tooth
Transgenes
zeta-Globins
Ameloblasts
Odontoblasts
Neoplasms
Glandular and Epithelial Neoplasms
ras Genes
Proliferating Cell Nuclear Antigen
Maxilla
Dentin
Dental Enamel
Mandible
Epithelial Cells
Immunohistochemistry
Incidence
Genes

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Otorhinolaryngology
  • Dentistry(all)

Cite this

Odontogenic tumours in the v-Ha-ras (TG · AC) transgenic mouse. / Wright, J. T.; Hansen, Laura A.; Mahler, J.; Szczesniak, C.; Spalding, J. W.

In: Archives of Oral Biology, Vol. 40, No. 7, 1995, p. 631-638.

Research output: Contribution to journalArticle

Wright, J. T. ; Hansen, Laura A. ; Mahler, J. ; Szczesniak, C. ; Spalding, J. W. / Odontogenic tumours in the v-Ha-ras (TG · AC) transgenic mouse. In: Archives of Oral Biology. 1995 ; Vol. 40, No. 7. pp. 631-638.
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