TY - JOUR
T1 - PAF receptors and G-proteins in human blood eosinophils and neutrophils
AU - Agrawal, Devendra K.
AU - Ali, N.
AU - Numao, T.
PY - 1992
Y1 - 1992
N2 - Identification of G-proteins and coupling of PAF receptors to G-proteins have been examined in the membranes of human blood eosinophils and neutrophils. Heterotrimeric G-proteins, G(i) and G(s), were present in both cell types, as demonstrated by immunoblotting and ADP-ribosylation with pertussis toxin. In addition, a group of low molecular mass (18-28 kDa) monomeric G-proteins was also identified. Pertussis toxin and GTPγS attenuated the specific binding of [3H]PAF, suggesting the occurrence of coupling between pertussis toxin-sensitive G(i) protein and PAF receptors in eosinophils and neutrophils.
AB - Identification of G-proteins and coupling of PAF receptors to G-proteins have been examined in the membranes of human blood eosinophils and neutrophils. Heterotrimeric G-proteins, G(i) and G(s), were present in both cell types, as demonstrated by immunoblotting and ADP-ribosylation with pertussis toxin. In addition, a group of low molecular mass (18-28 kDa) monomeric G-proteins was also identified. Pertussis toxin and GTPγS attenuated the specific binding of [3H]PAF, suggesting the occurrence of coupling between pertussis toxin-sensitive G(i) protein and PAF receptors in eosinophils and neutrophils.
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M3 - Article
C2 - 1326343
AN - SCOPUS:0026741243
VL - 5
SP - 101
EP - 104
JO - Journal of Lipid Mediators and Cell Signalling
JF - Journal of Lipid Mediators and Cell Signalling
SN - 1098-8823
IS - 2
ER -