PAF receptors and G-proteins in human blood eosinophils and neutrophils

Devendra K. Agrawal, N. Ali, T. Numao

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Identification of G-proteins and coupling of PAF receptors to G-proteins have been examined in the membranes of human blood eosinophils and neutrophils. Heterotrimeric G-proteins, G(i) and G(s), were present in both cell types, as demonstrated by immunoblotting and ADP-ribosylation with pertussis toxin. In addition, a group of low molecular mass (18-28 kDa) monomeric G-proteins was also identified. Pertussis toxin and GTPγS attenuated the specific binding of [3H]PAF, suggesting the occurrence of coupling between pertussis toxin-sensitive G(i) protein and PAF receptors in eosinophils and neutrophils.

Original languageEnglish
Pages (from-to)101-104
Number of pages4
JournalJournal of Lipid Mediators
Volume5
Issue number2
StatePublished - 1992

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Pertussis Toxin
GTP-Binding Proteins
Eosinophils
Neutrophils
Blood
Heterotrimeric GTP-Binding Proteins
Monomeric GTP-Binding Proteins
Molecular mass
Immunoblotting
Adenosine Diphosphate
Membranes
platelet activating factor receptor
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology
  • Pharmacology

Cite this

PAF receptors and G-proteins in human blood eosinophils and neutrophils. / Agrawal, Devendra K.; Ali, N.; Numao, T.

In: Journal of Lipid Mediators, Vol. 5, No. 2, 1992, p. 101-104.

Research output: Contribution to journalArticle

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