Abstract
Identification of G-proteins and coupling of PAF receptors to G-proteins have been examined in the membranes of human blood eosinophils and neutrophils. Heterotrimeric G-proteins, G(i) and G(s), were present in both cell types, as demonstrated by immunoblotting and ADP-ribosylation with pertussis toxin. In addition, a group of low molecular mass (18-28 kDa) monomeric G-proteins was also identified. Pertussis toxin and GTPγS attenuated the specific binding of [3H]PAF, suggesting the occurrence of coupling between pertussis toxin-sensitive G(i) protein and PAF receptors in eosinophils and neutrophils.
| Original language | English |
|---|---|
| Pages (from-to) | 101-104 |
| Number of pages | 4 |
| Journal | Journal of Lipid Mediators |
| Volume | 5 |
| Issue number | 2 |
| State | Published - 1992 |
All Science Journal Classification (ASJC) codes
- Immunology
- Pharmacology
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Agrawal, D. K., Ali, N., & Numao, T. (1992). PAF receptors and G-proteins in human blood eosinophils and neutrophils. Journal of Lipid Mediators, 5(2), 101-104.