Abstract
Identification of G-proteins and coupling of PAF receptors to G-proteins have been examined in the membranes of human blood eosinophils and neutrophils. Heterotrimeric G-proteins, G(i) and G(s), were present in both cell types, as demonstrated by immunoblotting and ADP-ribosylation with pertussis toxin. In addition, a group of low molecular mass (18-28 kDa) monomeric G-proteins was also identified. Pertussis toxin and GTPγS attenuated the specific binding of [3H]PAF, suggesting the occurrence of coupling between pertussis toxin-sensitive G(i) protein and PAF receptors in eosinophils and neutrophils.
| Original language | English |
|---|---|
| Pages (from-to) | 101-104 |
| Number of pages | 4 |
| Journal | Journal of Lipid Mediators |
| Volume | 5 |
| Issue number | 2 |
| State | Published - 1992 |
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All Science Journal Classification (ASJC) codes
- Immunology
- Pharmacology
Cite this
PAF receptors and G-proteins in human blood eosinophils and neutrophils. / Agrawal, Devendra K.; Ali, N.; Numao, T.
In: Journal of Lipid Mediators, Vol. 5, No. 2, 1992, p. 101-104.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - PAF receptors and G-proteins in human blood eosinophils and neutrophils
AU - Agrawal, Devendra K.
AU - Ali, N.
AU - Numao, T.
PY - 1992
Y1 - 1992
N2 - Identification of G-proteins and coupling of PAF receptors to G-proteins have been examined in the membranes of human blood eosinophils and neutrophils. Heterotrimeric G-proteins, G(i) and G(s), were present in both cell types, as demonstrated by immunoblotting and ADP-ribosylation with pertussis toxin. In addition, a group of low molecular mass (18-28 kDa) monomeric G-proteins was also identified. Pertussis toxin and GTPγS attenuated the specific binding of [3H]PAF, suggesting the occurrence of coupling between pertussis toxin-sensitive G(i) protein and PAF receptors in eosinophils and neutrophils.
AB - Identification of G-proteins and coupling of PAF receptors to G-proteins have been examined in the membranes of human blood eosinophils and neutrophils. Heterotrimeric G-proteins, G(i) and G(s), were present in both cell types, as demonstrated by immunoblotting and ADP-ribosylation with pertussis toxin. In addition, a group of low molecular mass (18-28 kDa) monomeric G-proteins was also identified. Pertussis toxin and GTPγS attenuated the specific binding of [3H]PAF, suggesting the occurrence of coupling between pertussis toxin-sensitive G(i) protein and PAF receptors in eosinophils and neutrophils.
UR - http://www.scopus.com/inward/record.url?scp=0026741243&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026741243&partnerID=8YFLogxK
M3 - Article
VL - 5
SP - 101
EP - 104
JO - Prostaglandins and Other Lipid Mediators
JF - Prostaglandins and Other Lipid Mediators
SN - 1098-8823
IS - 2
ER -