PAF receptors and G-proteins in human blood eosinophils and neutrophils

Devendra K. Agrawal, N. Ali, T. Numao

    Research output: Contribution to journalArticlepeer-review

    18 Scopus citations

    Abstract

    Identification of G-proteins and coupling of PAF receptors to G-proteins have been examined in the membranes of human blood eosinophils and neutrophils. Heterotrimeric G-proteins, G(i) and G(s), were present in both cell types, as demonstrated by immunoblotting and ADP-ribosylation with pertussis toxin. In addition, a group of low molecular mass (18-28 kDa) monomeric G-proteins was also identified. Pertussis toxin and GTPγS attenuated the specific binding of [3H]PAF, suggesting the occurrence of coupling between pertussis toxin-sensitive G(i) protein and PAF receptors in eosinophils and neutrophils.

    Original languageEnglish
    Pages (from-to)101-104
    Number of pages4
    JournalJournal of Lipid Mediators
    Volume5
    Issue number2
    StatePublished - 1992

    All Science Journal Classification (ASJC) codes

    • Immunology
    • Pharmacology

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