PAF receptors and G-proteins in human blood eosinophils and neutrophils

Devendra K. Agrawal, N. Ali, T. Numao

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Abstract

Identification of G-proteins and coupling of PAF receptors to G-proteins have been examined in the membranes of human blood eosinophils and neutrophils. Heterotrimeric G-proteins, G(i) and G(s), were present in both cell types, as demonstrated by immunoblotting and ADP-ribosylation with pertussis toxin. In addition, a group of low molecular mass (18-28 kDa) monomeric G-proteins was also identified. Pertussis toxin and GTPγS attenuated the specific binding of [3H]PAF, suggesting the occurrence of coupling between pertussis toxin-sensitive G(i) protein and PAF receptors in eosinophils and neutrophils.

Original languageEnglish
Pages (from-to)101-104
Number of pages4
JournalJournal of Lipid Mediators
Volume5
Issue number2
Publication statusPublished - 1992

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All Science Journal Classification (ASJC) codes

  • Immunology
  • Pharmacology

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