PCNA structure and function

insights from structures of PCNA complexes and post-translationally modified PCNA.

Lynne Dieckman, Bret D. Freudenthal, M. Todd Washington

Research output: Contribution to journalReview article

40 Citations (Scopus)

Abstract

Proliferating cell nuclear antigen (PCNA), the eukaryotic DNA sliding clamp, forms a ring-shaped homo-trimer that encircles double-stranded DNA. This protein is best known for its ability to confer high processivity to replicative DNA polymerases. However, it does far more than this, because it forms a mobile platform on the DNA that recruits many of the proteins involved in DNA replication, repair, and recombination to replication forks. X-ray crystal structures of PCNA bound to PCNA-binding proteins have provided insights into how PCNA recognizes its binding partners and recruits them to replication forks. More recently, X-ray crystal structures of ubiquitin-modified and SUMO-modified PCNA have provided insights into how these post-translational modifications alter the specificity of PCNA for some of its binding partners. This article focuses on the insights gained from structural studies of PCNA complexes and post-translationally modified PCNA.

Original languageEnglish
Pages (from-to)281-299
Number of pages19
JournalSub-Cellular Biochemistry
Volume62
StatePublished - 2012
Externally publishedYes

Fingerprint

Proliferating Cell Nuclear Antigen
DNA
Crystal structure
X-Rays
X rays
Clamping devices
DNA-Directed DNA Polymerase
Post Translational Protein Processing
Ubiquitin
DNA Replication
DNA Repair
Genetic Recombination
Carrier Proteins
Proteins
Repair

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

PCNA structure and function : insights from structures of PCNA complexes and post-translationally modified PCNA. / Dieckman, Lynne; Freudenthal, Bret D.; Washington, M. Todd.

In: Sub-Cellular Biochemistry, Vol. 62, 2012, p. 281-299.

Research output: Contribution to journalReview article

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