PCNA structure and function: Insights from structures of PCNA complexes and post-translationally modi fied PCNA

Lynne M. Dieckman, Bret D. Freudenthal, M. Todd Washington

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Proliferating cell nuclear antigen (PCNA), the eukaryotic DNA sliding clamp, forms a ring-shaped homo-trimer that encircles double-stranded DNA. This protein is best known for its ability to confer high processivity to replicative DNA polymerases. However, it does far more than this, because it forms a mobile platform on the DNA that recruits many of the proteins involved in DNA replication, repair, and recombination to replication forks. X-ray crystal structures of PCNA bound to PCNA-binding proteins have provided insights into how PCNA recognizes its binding partners and recruits them to replication forks. More recently, X-ray crystal structures of ubiquitin-modified and SUMO-modified PCNA have provided insights into how these post-translational modifications alter the specificity of PCNA for some of its binding partners. This article focuses on the insights gained from structural studies of PCNA complexes and post-translationally modified PCNA.

Original languageEnglish (US)
Pages (from-to)281-299
Number of pages19
JournalSubcellular Biochemistry
Volume62
DOIs
StatePublished - May 29 2012

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology
  • Cancer Research

Cite this