Abstract
Three peptides with growth-inhibitory activity towards the Gram-negative bacterium Eschericia coli were isolated from electrically stimulated secretions from the skin of the southern leopard frog, Rana sphenocephala. Structural characterization demonstrated that the peptides [brevinin-1Sa, minimum inhibitory concentration (MIC) = 55μM; brevinin-lSb, MIC = 17 μM; brevinin-lSc, MIC = 14 μM] represent new members of the brevinin-1 family of antimicrobial peptides, previously isolated from several other species of frogs of the genus Rana. Their high concentration in skin secretions and extreme variability in amino acid sequence suggest that the brevinin family of peptides may be of value as molecular markers for the identification and taxonomic classification of Ranid frogs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 522-527 |
| Number of pages | 6 |
| Journal | Journal of Peptide Research |
| Volume | 54 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 13 1999 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Endocrinology
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Peptides with antimicrobial activity of the brevinin-1 family isolated from skin secretions of the southern leopard frog, Rana sphenocephala. / Conlon, J. M.; Halverson, T.; Dulka, J.; Platz, J. E.; Knoop, Floyd C.
In: Journal of Peptide Research, Vol. 54, No. 6, 13.12.1999, p. 522-527.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Peptides with antimicrobial activity of the brevinin-1 family isolated from skin secretions of the southern leopard frog, Rana sphenocephala
AU - Conlon, J. M.
AU - Halverson, T.
AU - Dulka, J.
AU - Platz, J. E.
AU - Knoop, Floyd C.
PY - 1999/12/13
Y1 - 1999/12/13
N2 - Three peptides with growth-inhibitory activity towards the Gram-negative bacterium Eschericia coli were isolated from electrically stimulated secretions from the skin of the southern leopard frog, Rana sphenocephala. Structural characterization demonstrated that the peptides [brevinin-1Sa, minimum inhibitory concentration (MIC) = 55μM; brevinin-lSb, MIC = 17 μM; brevinin-lSc, MIC = 14 μM] represent new members of the brevinin-1 family of antimicrobial peptides, previously isolated from several other species of frogs of the genus Rana. Their high concentration in skin secretions and extreme variability in amino acid sequence suggest that the brevinin family of peptides may be of value as molecular markers for the identification and taxonomic classification of Ranid frogs.
AB - Three peptides with growth-inhibitory activity towards the Gram-negative bacterium Eschericia coli were isolated from electrically stimulated secretions from the skin of the southern leopard frog, Rana sphenocephala. Structural characterization demonstrated that the peptides [brevinin-1Sa, minimum inhibitory concentration (MIC) = 55μM; brevinin-lSb, MIC = 17 μM; brevinin-lSc, MIC = 14 μM] represent new members of the brevinin-1 family of antimicrobial peptides, previously isolated from several other species of frogs of the genus Rana. Their high concentration in skin secretions and extreme variability in amino acid sequence suggest that the brevinin family of peptides may be of value as molecular markers for the identification and taxonomic classification of Ranid frogs.
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UR - http://www.scopus.com/inward/citedby.url?scp=0032714837&partnerID=8YFLogxK
U2 - 10.1034/j.1399-3011.1999.00123.x
DO - 10.1034/j.1399-3011.1999.00123.x
M3 - Article
C2 - 10604597
AN - SCOPUS:0032714837
VL - 54
SP - 522
EP - 527
JO - Chemical Biology and Drug Design
JF - Chemical Biology and Drug Design
SN - 1747-0277
IS - 6
ER -