Peptidyl-glycine α-amidating monooxygenase is present in islet secretory granules of the anglerfish, Lophius americanus

Robert Mackin, Jonathan M. Flacker, Julie A. Mackin, Bryan D. Noe

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Anglerfish islet secretory granules have been examined for the presence of an enzyme which could perform C-terminal amidation of glucagon-like peptide II and possibly anglerfish peptide Y. Using [125I]d-Tyr-Val-Gly as substrate, a peptidyl-glycine α-amidating monooxygenase (PAM) was detected in islet secretory granule lysates. The enzyme is active between pH 6.0 and 8.5 and exhibits maximal activity near pH 7.0. The islet PAM requires Cu2+, ascorbate, and molecular oxygen for activity. Other divalent metal ions and redox cofactors were tested and found to be inactive in the assay. Even though added Cu2+ and ascorbate are required for detecting islet PAM activity, when these factors were incubated with substrate in the absence of secretory granule lysate, no activity was observed. It was also found that the addition of higher than optimal concentrations of either Cu2+ or ascorbate inhibited amidating activity. The results demonstrate that a PAM is present in secretory granules of anglerfish islet tissue. The characteristics of the islet PAM are similar to those of PAMs identified and characterized in other tissues which produce bioactive C-terminally amidated peptides.

Original languageEnglish
Pages (from-to)263-269
Number of pages7
JournalGeneral and Comparative Endocrinology
Volume67
Issue number2
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Lophius americanus
secretory granules
Secretory Vesicles
Mixed Function Oxygenases
Glycine
peptides
Glucagon-Like Peptides
enzymes
metal ions
Peptides
Enzymes
Oxidation-Reduction
oxygen
Metals
Ions
Oxygen
assays

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Peptidyl-glycine α-amidating monooxygenase is present in islet secretory granules of the anglerfish, Lophius americanus. / Mackin, Robert; Flacker, Jonathan M.; Mackin, Julie A.; Noe, Bryan D.

In: General and Comparative Endocrinology, Vol. 67, No. 2, 1987, p. 263-269.

Research output: Contribution to journalArticle

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