Pertussis toxin in the A10 region increases dopamine synthesis and metabolism

Jeffery D. Steketee, Caryn D. Striplin, Thomas F. Murray, Peter W. Kalivas

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Inhibitory regulation of dopamine neurons is mediated by dopamine autoreceptor and γ-aminobutyric acidB receptor opening of potassium channels. Increased potassium conductance by either receptor is G protein dependent. To evaluate the role of G proteins in vivo, pertussis toxin (PTX) was microinjected into the A10 dopamine region and changes in dopamine metabolism and synthesis measured. PTX produced an elevation in dopamine metabolism and synthesis in the A10 region and nucleus accumbens for up to 4 days after injection. By day 7 the levels of the dopamine precursor and metabolites had returned to normal. A less consistent increase was also measured in the A9 dopamine region and the prefrontal cortex. Although dopamine synthesis and metabolism had returned to normal by day 7, the in vitro ADP-ribosylation of G proteins in the A10 region by PTX remained depressed by approximately 50% from day 1 to day 14 after administration, returning to normal by day 30. The data suggest that in vivo ribosylation of G proteins may lead to a short-term attenuation of the tonic inhibitory control of dopamine neurons, which can be compensated for by PTX-insensitive mechanisms.

Original languageEnglish
Pages (from-to)811-816
Number of pages6
JournalJournal of Neurochemistry
Volume58
Issue number3
StatePublished - Mar 1992
Externally publishedYes

Fingerprint

antineoplaston A10
Pertussis Toxin
Metabolism
Dopamine
GTP-Binding Proteins
Dopaminergic Neurons
Neurons
Autoreceptors
Potassium Channels
Nucleus Accumbens
Prefrontal Cortex
Adenosine Diphosphate
Potassium
Metabolites
Injections

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Steketee, J. D., Striplin, C. D., Murray, T. F., & Kalivas, P. W. (1992). Pertussis toxin in the A10 region increases dopamine synthesis and metabolism. Journal of Neurochemistry, 58(3), 811-816.

Pertussis toxin in the A10 region increases dopamine synthesis and metabolism. / Steketee, Jeffery D.; Striplin, Caryn D.; Murray, Thomas F.; Kalivas, Peter W.

In: Journal of Neurochemistry, Vol. 58, No. 3, 03.1992, p. 811-816.

Research output: Contribution to journalArticle

Steketee, JD, Striplin, CD, Murray, TF & Kalivas, PW 1992, 'Pertussis toxin in the A10 region increases dopamine synthesis and metabolism', Journal of Neurochemistry, vol. 58, no. 3, pp. 811-816.
Steketee, Jeffery D. ; Striplin, Caryn D. ; Murray, Thomas F. ; Kalivas, Peter W. / Pertussis toxin in the A10 region increases dopamine synthesis and metabolism. In: Journal of Neurochemistry. 1992 ; Vol. 58, No. 3. pp. 811-816.
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