Abstract
Lipophilic fluorescent dyes have been used to trace neuronal connections because of their ability to diffuse laterally within nerve cell membranes. Given the hundreds to thousands of connections that a typical neuron makes with its neighbours, a diffusion-matched set of spectrally distinct dyes is desirable. To extend a set of these dyes to obtain six independent labels, we have characterized the properties of novel violet and near-infrared candidates. By combining two-photon and confocal microscopy all of these candidates can be imaged using a single Titanium Sapphire laser. Here we present measurements of the two-photon action cross-sections and diffusion properties of the dyes, using either the relative diffusion distance or fluorescence recovery after photobleaching techniques, and demonstrate six-colour neuronal tracing within the spinal cord and brain tissue.
Original language | English (US) |
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Pages (from-to) | 117-134 |
Number of pages | 18 |
Journal | Journal of Microscopy |
Volume | 239 |
Issue number | 2 |
DOIs | |
State | Published - Aug 2010 |
All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Histology