Abstract
Lipophilic fluorescent dyes have been used to trace neuronal connections because of their ability to diffuse laterally within nerve cell membranes. Given the hundreds to thousands of connections that a typical neuron makes with its neighbours, a diffusion-matched set of spectrally distinct dyes is desirable. To extend a set of these dyes to obtain six independent labels, we have characterized the properties of novel violet and near-infrared candidates. By combining two-photon and confocal microscopy all of these candidates can be imaged using a single Titanium Sapphire laser. Here we present measurements of the two-photon action cross-sections and diffusion properties of the dyes, using either the relative diffusion distance or fluorescence recovery after photobleaching techniques, and demonstrate six-colour neuronal tracing within the spinal cord and brain tissue.
| Original language | English |
|---|---|
| Pages (from-to) | 117-134 |
| Number of pages | 18 |
| Journal | Journal of Microscopy |
| Volume | 239 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2010 |
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All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Histology
- Medicine(all)
Cite this
Photo- and bio-physical characterization of novel violet and near-infrared lipophilic fluorophores for neuronal tracing. / Tonniges, J.; Hansen, M.; Duncan, J.; Bassett, M. J.; Fritzsch, B.; Gray, B. D.; Easwaran, A.; Nichols, Michael G.
In: Journal of Microscopy, Vol. 239, No. 2, 2010, p. 117-134.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Photo- and bio-physical characterization of novel violet and near-infrared lipophilic fluorophores for neuronal tracing
AU - Tonniges, J.
AU - Hansen, M.
AU - Duncan, J.
AU - Bassett, M. J.
AU - Fritzsch, B.
AU - Gray, B. D.
AU - Easwaran, A.
AU - Nichols, Michael G.
PY - 2010
Y1 - 2010
N2 - Lipophilic fluorescent dyes have been used to trace neuronal connections because of their ability to diffuse laterally within nerve cell membranes. Given the hundreds to thousands of connections that a typical neuron makes with its neighbours, a diffusion-matched set of spectrally distinct dyes is desirable. To extend a set of these dyes to obtain six independent labels, we have characterized the properties of novel violet and near-infrared candidates. By combining two-photon and confocal microscopy all of these candidates can be imaged using a single Titanium Sapphire laser. Here we present measurements of the two-photon action cross-sections and diffusion properties of the dyes, using either the relative diffusion distance or fluorescence recovery after photobleaching techniques, and demonstrate six-colour neuronal tracing within the spinal cord and brain tissue.
AB - Lipophilic fluorescent dyes have been used to trace neuronal connections because of their ability to diffuse laterally within nerve cell membranes. Given the hundreds to thousands of connections that a typical neuron makes with its neighbours, a diffusion-matched set of spectrally distinct dyes is desirable. To extend a set of these dyes to obtain six independent labels, we have characterized the properties of novel violet and near-infrared candidates. By combining two-photon and confocal microscopy all of these candidates can be imaged using a single Titanium Sapphire laser. Here we present measurements of the two-photon action cross-sections and diffusion properties of the dyes, using either the relative diffusion distance or fluorescence recovery after photobleaching techniques, and demonstrate six-colour neuronal tracing within the spinal cord and brain tissue.
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UR - http://www.scopus.com/inward/citedby.url?scp=77954714418&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2818.2009.03363.x
DO - 10.1111/j.1365-2818.2009.03363.x
M3 - Article
VL - 239
SP - 117
EP - 134
JO - Journal of Microscopy
JF - Journal of Microscopy
SN - 0022-2720
IS - 2
ER -