Abstract
Concentrated cell-free filtrates, prepared from a human strain of enterotoxigenic Escherichia coli, were subjected to isoelectric focusing, molecular sieve chromatography, and polyacrylamide disc gel electrophoresis. Isoelectric focusing in a pH 3 to 5 gradient resulted in two biologically active peaks, I and II, that electrofocused at pI 1.5 and 3.8, respectively. Molecular sieve chromatography of the major enterotoxic peak (II) at pH 3.8 indicated a molecular weight of 2,500. Polyacrylamide disc gel electrophoresis of ST revealed a single protein band containing enterotoxic activity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1051-1053 |
| Number of pages | 3 |
| Journal | Infection and Immunity |
| Volume | 28 |
| Issue number | 3 |
| State | Published - Jan 1 1980 |
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All Science Journal Classification (ASJC) codes
- Immunology
Cite this
Physiochemical properties of a heat-stable enterotoxin produced by Escherichia coli of human origin. / Madsen, G. L.; Knoop, Floyd C.
In: Infection and Immunity, Vol. 28, No. 3, 01.01.1980, p. 1051-1053.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Physiochemical properties of a heat-stable enterotoxin produced by Escherichia coli of human origin
AU - Madsen, G. L.
AU - Knoop, Floyd C.
PY - 1980/1/1
Y1 - 1980/1/1
N2 - Concentrated cell-free filtrates, prepared from a human strain of enterotoxigenic Escherichia coli, were subjected to isoelectric focusing, molecular sieve chromatography, and polyacrylamide disc gel electrophoresis. Isoelectric focusing in a pH 3 to 5 gradient resulted in two biologically active peaks, I and II, that electrofocused at pI 1.5 and 3.8, respectively. Molecular sieve chromatography of the major enterotoxic peak (II) at pH 3.8 indicated a molecular weight of 2,500. Polyacrylamide disc gel electrophoresis of ST revealed a single protein band containing enterotoxic activity.
AB - Concentrated cell-free filtrates, prepared from a human strain of enterotoxigenic Escherichia coli, were subjected to isoelectric focusing, molecular sieve chromatography, and polyacrylamide disc gel electrophoresis. Isoelectric focusing in a pH 3 to 5 gradient resulted in two biologically active peaks, I and II, that electrofocused at pI 1.5 and 3.8, respectively. Molecular sieve chromatography of the major enterotoxic peak (II) at pH 3.8 indicated a molecular weight of 2,500. Polyacrylamide disc gel electrophoresis of ST revealed a single protein band containing enterotoxic activity.
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UR - http://www.scopus.com/inward/citedby.url?scp=0018869977&partnerID=8YFLogxK
M3 - Article
C2 - 6995319
AN - SCOPUS:0018869977
VL - 28
SP - 1051
EP - 1053
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 3
ER -