Abstract
Concentrated cell-free filtrates, prepared from a human strain of enterotoxigenic Escherichia coli, were subjected to isoelectric focusing, molecular sieve chromatography, and polyacrylamide disc gel electrophoresis. Isoelectric focusing in a pH 3 to 5 gradient resulted in two biologically active peaks, I and II, that electrofocused at pI 1.5 and 3.8, respectively. Molecular sieve chromatography of the major enterotoxic peak (II) at pH 3.8 indicated a molecular weight of 2,500. Polyacrylamide disc gel electrophoresis of ST revealed a single protein band containing enterotoxic activity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1051-1053 |
| Number of pages | 3 |
| Journal | Infection and Immunity |
| Volume | 28 |
| Issue number | 3 |
| Publication status | Published - Jan 1 1980 |
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All Science Journal Classification (ASJC) codes
- Immunology
Cite this
Madsen, G. L., & Knoop, F. C. (1980). Physiochemical properties of a heat-stable enterotoxin produced by Escherichia coli of human origin. Infection and Immunity, 28(3), 1051-1053.