PKCγ regulates syndecan-2 inside-out signaling during xenopus left-right development

Ken Kramer, Janet E. Barnette, H. Joseph Yost

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

The transmembrane proteoglycan syndecan-2 cell nonautonomously regulates left-right (LR) development in migrating mesoderm by an unknown mechanism, leading to LR asymmetric gene expression and LR orientation of the heart and gut. Here, we demonstrate that protein kinase C γ (PKCγ) mediates phosphorylation of the cytoplasmic domain of syndecan-2 in right, but not left, animal cap ectodermal cells. Notably, both phosphorylation states of syndecan-2 are obligatory for normal LR development, with PKCγ-dependent phosphorylated syndecan-2 in right ectodermal cells and nonphosphorylated syndecan-2 in left cells. The ectodermal cells contact migrating mesodermal cells during early gastrulation, concurrent with the transmission of LR information. This precedes the appearance of monocilia and is one of the earliest steps of LR development. These results demonstrate that PKCγ regulates the cytoplasmic phosphorylation of syndecan-2 and, consequently, syndecan-2-mediated inside-out signaling to adjacent cells.

Original languageEnglish
Pages (from-to)981-990
Number of pages10
JournalCell
Volume111
Issue number7
DOIs
StatePublished - Dec 27 2002
Externally publishedYes

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Syndecan-2
Xenopus
Protein Kinase C
Phosphorylation
Gastrulation
Mesoderm
Proteoglycans
Gene expression
Animals
Gene Expression

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Molecular Biology

Cite this

PKCγ regulates syndecan-2 inside-out signaling during xenopus left-right development. / Kramer, Ken; Barnette, Janet E.; Yost, H. Joseph.

In: Cell, Vol. 111, No. 7, 27.12.2002, p. 981-990.

Research output: Contribution to journalArticle

Kramer, Ken ; Barnette, Janet E. ; Yost, H. Joseph. / PKCγ regulates syndecan-2 inside-out signaling during xenopus left-right development. In: Cell. 2002 ; Vol. 111, No. 7. pp. 981-990.
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