PKCγ regulates syndecan-2 inside-out signaling during xenopus left-right development

Kenneth L. Kramer; Janet E. Barnette; H. Joseph Yost

doi:10.1016/S0092-8674(02)01200-X

PKCγ regulates syndecan-2 inside-out signaling during xenopus left-right development

Kenneth L. Kramer, Janet E. Barnette, H. Joseph Yost

Research output: Contribution to journal › Article

93 Scopus citations

Abstract

The transmembrane proteoglycan syndecan-2 cell nonautonomously regulates left-right (LR) development in migrating mesoderm by an unknown mechanism, leading to LR asymmetric gene expression and LR orientation of the heart and gut. Here, we demonstrate that protein kinase C γ (PKCγ) mediates phosphorylation of the cytoplasmic domain of syndecan-2 in right, but not left, animal cap ectodermal cells. Notably, both phosphorylation states of syndecan-2 are obligatory for normal LR development, with PKCγ-dependent phosphorylated syndecan-2 in right ectodermal cells and nonphosphorylated syndecan-2 in left cells. The ectodermal cells contact migrating mesodermal cells during early gastrulation, concurrent with the transmission of LR information. This precedes the appearance of monocilia and is one of the earliest steps of LR development. These results demonstrate that PKCγ regulates the cytoplasmic phosphorylation of syndecan-2 and, consequently, syndecan-2-mediated inside-out signaling to adjacent cells.

Original language	English (US)
Pages (from-to)	981-990
Number of pages	10
Journal	Cell
Volume	111
Issue number	7
DOIs	https://doi.org/10.1016/S0092-8674(02)01200-X
State	Published - Dec 27 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.1016/S0092-8674(02)01200-X

Fingerprint Dive into the research topics of 'PKCγ regulates syndecan-2 inside-out signaling during xenopus left-right development'. Together they form a unique fingerprint.

Cite this

Kramer, K. L., Barnette, J. E., & Yost, H. J. (2002). PKCγ regulates syndecan-2 inside-out signaling during xenopus left-right development. Cell, 111(7), 981-990. https://doi.org/10.1016/S0092-8674(02)01200-X

@article{7addaea61c62496a843eeb827f8360e2,

title = "PKCγ regulates syndecan-2 inside-out signaling during xenopus left-right development",

abstract = "The transmembrane proteoglycan syndecan-2 cell nonautonomously regulates left-right (LR) development in migrating mesoderm by an unknown mechanism, leading to LR asymmetric gene expression and LR orientation of the heart and gut. Here, we demonstrate that protein kinase C γ (PKCγ) mediates phosphorylation of the cytoplasmic domain of syndecan-2 in right, but not left, animal cap ectodermal cells. Notably, both phosphorylation states of syndecan-2 are obligatory for normal LR development, with PKCγ-dependent phosphorylated syndecan-2 in right ectodermal cells and nonphosphorylated syndecan-2 in left cells. The ectodermal cells contact migrating mesodermal cells during early gastrulation, concurrent with the transmission of LR information. This precedes the appearance of monocilia and is one of the earliest steps of LR development. These results demonstrate that PKCγ regulates the cytoplasmic phosphorylation of syndecan-2 and, consequently, syndecan-2-mediated inside-out signaling to adjacent cells.",

author = "Kramer, {Kenneth L.} and Barnette, {Janet E.} and Yost, {H. Joseph}",

year = "2002",

month = dec,

day = "27",

doi = "10.1016/S0092-8674(02)01200-X",

language = "English (US)",

volume = "111",

pages = "981--990",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "7",

}

TY - JOUR

T1 - PKCγ regulates syndecan-2 inside-out signaling during xenopus left-right development

AU - Kramer, Kenneth L.

AU - Barnette, Janet E.

AU - Yost, H. Joseph

PY - 2002/12/27

Y1 - 2002/12/27

N2 - The transmembrane proteoglycan syndecan-2 cell nonautonomously regulates left-right (LR) development in migrating mesoderm by an unknown mechanism, leading to LR asymmetric gene expression and LR orientation of the heart and gut. Here, we demonstrate that protein kinase C γ (PKCγ) mediates phosphorylation of the cytoplasmic domain of syndecan-2 in right, but not left, animal cap ectodermal cells. Notably, both phosphorylation states of syndecan-2 are obligatory for normal LR development, with PKCγ-dependent phosphorylated syndecan-2 in right ectodermal cells and nonphosphorylated syndecan-2 in left cells. The ectodermal cells contact migrating mesodermal cells during early gastrulation, concurrent with the transmission of LR information. This precedes the appearance of monocilia and is one of the earliest steps of LR development. These results demonstrate that PKCγ regulates the cytoplasmic phosphorylation of syndecan-2 and, consequently, syndecan-2-mediated inside-out signaling to adjacent cells.

AB - The transmembrane proteoglycan syndecan-2 cell nonautonomously regulates left-right (LR) development in migrating mesoderm by an unknown mechanism, leading to LR asymmetric gene expression and LR orientation of the heart and gut. Here, we demonstrate that protein kinase C γ (PKCγ) mediates phosphorylation of the cytoplasmic domain of syndecan-2 in right, but not left, animal cap ectodermal cells. Notably, both phosphorylation states of syndecan-2 are obligatory for normal LR development, with PKCγ-dependent phosphorylated syndecan-2 in right ectodermal cells and nonphosphorylated syndecan-2 in left cells. The ectodermal cells contact migrating mesodermal cells during early gastrulation, concurrent with the transmission of LR information. This precedes the appearance of monocilia and is one of the earliest steps of LR development. These results demonstrate that PKCγ regulates the cytoplasmic phosphorylation of syndecan-2 and, consequently, syndecan-2-mediated inside-out signaling to adjacent cells.

UR - http://www.scopus.com/inward/record.url?scp=0037184611&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037184611&partnerID=8YFLogxK

U2 - 10.1016/S0092-8674(02)01200-X

DO - 10.1016/S0092-8674(02)01200-X

M3 - Article

C2 - 12507425

AN - SCOPUS:0037184611

VL - 111

SP - 981

EP - 990

JO - Cell

JF - Cell

SN - 0092-8674

IS - 7

ER -