PLCγ-activated signalling is essential for TrkB mediated sensory neuron structural plasticity

Carla Sciarretta, Bernd Fritzsch, Kirk Beisel, Sonia Rocha-Sanchez, Annalisa Buniello, Jacqueline M. Horn, Liliana Minichiello

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background. The vestibular system provides the primary input of our sense of balance and spatial orientation. Dysfunction of the vestibular system can severely affect a person's quality of life. Therefore, understanding the molecular basis of vestibular neuron survival, maintenance, and innervation of the target sensory epithelia is fundamental. Results. Here we report that a point mutation at the phospholipase C (PLC) docking site in the mouse neurotrophin tyrosine kinase receptor TrkB (Ntrk2) specifically impairs fiber guidance inside the vestibular sensory epithelia, but has limited effects on the survival of vestibular sensory neurons and growth of afferent processes toward the sensory epithelia. We also show that expression of the TRPC3 cation calcium channel, whose activity is known to be required for nerve-growth cone guidance induced by brain-derived neurotrophic factor (BDNF), is altered in these animals. In addition, we find that absence of the PLC mediated TrkB signalling interferes with the transformation of bouton type afferent terminals of vestibular dendrites into calyces (the largest synaptic contact of dendrites known in the mammalian nervous system) on type I vestibular hair cells; the latter are normally distributed in these mutants as revealed by an unaltered expression pattern of the potassium channel KCNQ4 in these cells. Conclusions. These results demonstrate a crucial involvement of the TrkB/PLC-mediated intracellular signalling in structural aspects of sensory neuron plasticity.

Original languageEnglish
Article number103
JournalBMC Developmental Biology
Volume10
DOIs
StatePublished - 2010

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Type C Phospholipases
Sensory Receptor Cells
Epithelium
Dendrites
Vestibular Hair Cells
Growth Cones
Potassium Channels
Brain-Derived Neurotrophic Factor
Nerve Growth Factors
Receptor Protein-Tyrosine Kinases
Calcium Channels
Point Mutation
Nervous System
Maintenance
Quality of Life
Neurons
Growth

All Science Journal Classification (ASJC) codes

  • Developmental Biology

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PLCγ-activated signalling is essential for TrkB mediated sensory neuron structural plasticity. / Sciarretta, Carla; Fritzsch, Bernd; Beisel, Kirk; Rocha-Sanchez, Sonia; Buniello, Annalisa; Horn, Jacqueline M.; Minichiello, Liliana.

In: BMC Developmental Biology, Vol. 10, 103, 2010.

Research output: Contribution to journalArticle

Sciarretta, Carla ; Fritzsch, Bernd ; Beisel, Kirk ; Rocha-Sanchez, Sonia ; Buniello, Annalisa ; Horn, Jacqueline M. ; Minichiello, Liliana. / PLCγ-activated signalling is essential for TrkB mediated sensory neuron structural plasticity. In: BMC Developmental Biology. 2010 ; Vol. 10.
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