Prejunctional α2-adrenoceptors and peroxide-induced potentiation of norepinephrine release from the bovine iris

Catherine A. Opere, Sunny E. Ohia

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Peroxides can enhance field-stimulated [3H]norepinephrine ([3H]NE) release in isolated irides from several mammalian species. In the present study, we investigated the role of prejunctional α2-adrenoceptors in peroxide-induced potentiation of sympathetic neurotransmission in bovine isolated irides. Isolated hemi-irides were incubated in a Krebs buffered- solution containing [3H]NE and prepared for studies of neurotransmitter release using the superfusion method. α2-Adrenoceptor agonists, oxymetazoline, UK-14304 and clonidine inhibited field-stimulated [3H]NE overflow without affecting basal tritium efflux. Pretreatment of tissues with H2O2 (300 μM) had no effect on inhibition of evoked [3H]NE release caused by the α2-adrenergic agonists. However, H2O2 (300 μM) caused significant (P <0.01) leftward shifts of excitatory concentration-response curves to yohimbine (10 nM-1 μM). In contrast, yohimbine (1 μM) did not prevent the enhancement of evoked [3H]NE overflow induced by H2O2 (300 μM). In conclusion, excitatory effects of peroxides on sympathetic neurotransmission in bovine irides are not mediated by prejunctional α2-adrenoceptors.

Original languageEnglish (US)
Pages (from-to)1093-1098
Number of pages6
JournalNeurochemical Research
Volume23
Issue number8
DOIs
StatePublished - Aug 8 1998

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

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