TY - JOUR
T1 - Prejunctional inhibitory effects of isoprostanes on dopaminergic neurotransmission in bovine retinae, in vitro
AU - Liu, Hong
AU - Zhao, Min
AU - Opere, Catherine A.
PY - 2008/1
Y1 - 2008/1
N2 - We investigated the effect of isoprostanes (IsoPs) on potassium (K +)-depolarization-evoked release of [3H]dopamine from isolated bovine retinae. Isolated retinae were preloaded with [ 3H]dopamine and then prepared for studies of [3H]dopamine release using the superfusion method. 8-iso(15R)PGF2α, 8-isoPGE2, 8-isoPGE1 and 8-isoPGF2α attenuated [3H]dopamine release from isolated bovine retinae. At a concentration of 1 μM, the rank order of activity displayed by IsoP agonists was: 8-iso(15R)PGF2α > 8-isoPGE2 > 8-isoPGE1 > 8-isoPGF2α. Inhibition of cyclooxygenase (COX) with flurbiprofen reversed the effects caused by 8-isoPGE2 (10 nM and 10 μM), 8-iso(15R)PGF2α (1 μM) and 8-isoPGE1 (1 μM). Although the EP1/EP2 antagonist, AH 6809 (10 μM) had no significant effect on K+-induced [ 3H]dopamine release, it blocked the inhibitory effect of both 8-isoPGE1 (10 μM) and 8-isoPGE2 (10 μM). In conclusion, IsoPs attenuate K+-induced [3H]dopamine release in isolated bovine retinae, presumably via an indirect action on COX pathway leading to the production of prostanoids, which in turn, activates EP receptors.
AB - We investigated the effect of isoprostanes (IsoPs) on potassium (K +)-depolarization-evoked release of [3H]dopamine from isolated bovine retinae. Isolated retinae were preloaded with [ 3H]dopamine and then prepared for studies of [3H]dopamine release using the superfusion method. 8-iso(15R)PGF2α, 8-isoPGE2, 8-isoPGE1 and 8-isoPGF2α attenuated [3H]dopamine release from isolated bovine retinae. At a concentration of 1 μM, the rank order of activity displayed by IsoP agonists was: 8-iso(15R)PGF2α > 8-isoPGE2 > 8-isoPGE1 > 8-isoPGF2α. Inhibition of cyclooxygenase (COX) with flurbiprofen reversed the effects caused by 8-isoPGE2 (10 nM and 10 μM), 8-iso(15R)PGF2α (1 μM) and 8-isoPGE1 (1 μM). Although the EP1/EP2 antagonist, AH 6809 (10 μM) had no significant effect on K+-induced [ 3H]dopamine release, it blocked the inhibitory effect of both 8-isoPGE1 (10 μM) and 8-isoPGE2 (10 μM). In conclusion, IsoPs attenuate K+-induced [3H]dopamine release in isolated bovine retinae, presumably via an indirect action on COX pathway leading to the production of prostanoids, which in turn, activates EP receptors.
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U2 - 10.1007/s11064-007-9404-z
DO - 10.1007/s11064-007-9404-z
M3 - Article
C2 - 17674205
AN - SCOPUS:36949001117
VL - 33
SP - 37
EP - 42
JO - Neurochemical Research
JF - Neurochemical Research
SN - 0364-3190
IS - 1
ER -