Prejunctional inhibitory effects of isoprostanes on dopaminergic neurotransmission in bovine retinae, in vitro

Hong Liu, Min Zhao, Catherine A. Opere

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We investigated the effect of isoprostanes (IsoPs) on potassium (K +)-depolarization-evoked release of [3H]dopamine from isolated bovine retinae. Isolated retinae were preloaded with [ 3H]dopamine and then prepared for studies of [3H]dopamine release using the superfusion method. 8-iso(15R)PGF, 8-isoPGE2, 8-isoPGE1 and 8-isoPGF attenuated [3H]dopamine release from isolated bovine retinae. At a concentration of 1 μM, the rank order of activity displayed by IsoP agonists was: 8-iso(15R)PGF > 8-isoPGE2 > 8-isoPGE1 > 8-isoPGF. Inhibition of cyclooxygenase (COX) with flurbiprofen reversed the effects caused by 8-isoPGE2 (10 nM and 10 μM), 8-iso(15R)PGF (1 μM) and 8-isoPGE1 (1 μM). Although the EP1/EP2 antagonist, AH 6809 (10 μM) had no significant effect on K+-induced [ 3H]dopamine release, it blocked the inhibitory effect of both 8-isoPGE1 (10 μM) and 8-isoPGE2 (10 μM). In conclusion, IsoPs attenuate K+-induced [3H]dopamine release in isolated bovine retinae, presumably via an indirect action on COX pathway leading to the production of prostanoids, which in turn, activates EP receptors.

Original languageEnglish
Pages (from-to)37-42
Number of pages6
JournalNeurochemical Research
Volume33
Issue number1
DOIs
StatePublished - Jan 2008

Fingerprint

Isoprostanes
Synaptic Transmission
Retina
Dopamine
Dinoprost
Prostaglandin-Endoperoxide Synthases
Flurbiprofen
Depolarization
Prostaglandins
In Vitro Techniques
Potassium

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry

Cite this

Prejunctional inhibitory effects of isoprostanes on dopaminergic neurotransmission in bovine retinae, in vitro. / Liu, Hong; Zhao, Min; Opere, Catherine A.

In: Neurochemical Research, Vol. 33, No. 1, 01.2008, p. 37-42.

Research output: Contribution to journalArticle

@article{dae209560c324d7ba683c2e9a2e7dd4b,
title = "Prejunctional inhibitory effects of isoprostanes on dopaminergic neurotransmission in bovine retinae, in vitro",
abstract = "We investigated the effect of isoprostanes (IsoPs) on potassium (K +)-depolarization-evoked release of [3H]dopamine from isolated bovine retinae. Isolated retinae were preloaded with [ 3H]dopamine and then prepared for studies of [3H]dopamine release using the superfusion method. 8-iso(15R)PGF2α, 8-isoPGE2, 8-isoPGE1 and 8-isoPGF2α attenuated [3H]dopamine release from isolated bovine retinae. At a concentration of 1 μM, the rank order of activity displayed by IsoP agonists was: 8-iso(15R)PGF2α > 8-isoPGE2 > 8-isoPGE1 > 8-isoPGF2α. Inhibition of cyclooxygenase (COX) with flurbiprofen reversed the effects caused by 8-isoPGE2 (10 nM and 10 μM), 8-iso(15R)PGF2α (1 μM) and 8-isoPGE1 (1 μM). Although the EP1/EP2 antagonist, AH 6809 (10 μM) had no significant effect on K+-induced [ 3H]dopamine release, it blocked the inhibitory effect of both 8-isoPGE1 (10 μM) and 8-isoPGE2 (10 μM). In conclusion, IsoPs attenuate K+-induced [3H]dopamine release in isolated bovine retinae, presumably via an indirect action on COX pathway leading to the production of prostanoids, which in turn, activates EP receptors.",
author = "Hong Liu and Min Zhao and Opere, {Catherine A.}",
year = "2008",
month = "1",
doi = "10.1007/s11064-007-9404-z",
language = "English",
volume = "33",
pages = "37--42",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - Prejunctional inhibitory effects of isoprostanes on dopaminergic neurotransmission in bovine retinae, in vitro

AU - Liu, Hong

AU - Zhao, Min

AU - Opere, Catherine A.

PY - 2008/1

Y1 - 2008/1

N2 - We investigated the effect of isoprostanes (IsoPs) on potassium (K +)-depolarization-evoked release of [3H]dopamine from isolated bovine retinae. Isolated retinae were preloaded with [ 3H]dopamine and then prepared for studies of [3H]dopamine release using the superfusion method. 8-iso(15R)PGF2α, 8-isoPGE2, 8-isoPGE1 and 8-isoPGF2α attenuated [3H]dopamine release from isolated bovine retinae. At a concentration of 1 μM, the rank order of activity displayed by IsoP agonists was: 8-iso(15R)PGF2α > 8-isoPGE2 > 8-isoPGE1 > 8-isoPGF2α. Inhibition of cyclooxygenase (COX) with flurbiprofen reversed the effects caused by 8-isoPGE2 (10 nM and 10 μM), 8-iso(15R)PGF2α (1 μM) and 8-isoPGE1 (1 μM). Although the EP1/EP2 antagonist, AH 6809 (10 μM) had no significant effect on K+-induced [ 3H]dopamine release, it blocked the inhibitory effect of both 8-isoPGE1 (10 μM) and 8-isoPGE2 (10 μM). In conclusion, IsoPs attenuate K+-induced [3H]dopamine release in isolated bovine retinae, presumably via an indirect action on COX pathway leading to the production of prostanoids, which in turn, activates EP receptors.

AB - We investigated the effect of isoprostanes (IsoPs) on potassium (K +)-depolarization-evoked release of [3H]dopamine from isolated bovine retinae. Isolated retinae were preloaded with [ 3H]dopamine and then prepared for studies of [3H]dopamine release using the superfusion method. 8-iso(15R)PGF2α, 8-isoPGE2, 8-isoPGE1 and 8-isoPGF2α attenuated [3H]dopamine release from isolated bovine retinae. At a concentration of 1 μM, the rank order of activity displayed by IsoP agonists was: 8-iso(15R)PGF2α > 8-isoPGE2 > 8-isoPGE1 > 8-isoPGF2α. Inhibition of cyclooxygenase (COX) with flurbiprofen reversed the effects caused by 8-isoPGE2 (10 nM and 10 μM), 8-iso(15R)PGF2α (1 μM) and 8-isoPGE1 (1 μM). Although the EP1/EP2 antagonist, AH 6809 (10 μM) had no significant effect on K+-induced [ 3H]dopamine release, it blocked the inhibitory effect of both 8-isoPGE1 (10 μM) and 8-isoPGE2 (10 μM). In conclusion, IsoPs attenuate K+-induced [3H]dopamine release in isolated bovine retinae, presumably via an indirect action on COX pathway leading to the production of prostanoids, which in turn, activates EP receptors.

UR - http://www.scopus.com/inward/record.url?scp=36949001117&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36949001117&partnerID=8YFLogxK

U2 - 10.1007/s11064-007-9404-z

DO - 10.1007/s11064-007-9404-z

M3 - Article

VL - 33

SP - 37

EP - 42

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 1

ER -