Prejunctional inhibitory effects of isoprostanes on dopaminergic neurotransmission in bovine retinae, in vitro

Hong Liu, Min Zhao, Catherine A. Opere

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Abstract

We investigated the effect of isoprostanes (IsoPs) on potassium (K +)-depolarization-evoked release of [3H]dopamine from isolated bovine retinae. Isolated retinae were preloaded with [ 3H]dopamine and then prepared for studies of [3H]dopamine release using the superfusion method. 8-iso(15R)PGF, 8-isoPGE2, 8-isoPGE1 and 8-isoPGF attenuated [3H]dopamine release from isolated bovine retinae. At a concentration of 1 μM, the rank order of activity displayed by IsoP agonists was: 8-iso(15R)PGF > 8-isoPGE2 > 8-isoPGE1 > 8-isoPGF. Inhibition of cyclooxygenase (COX) with flurbiprofen reversed the effects caused by 8-isoPGE2 (10 nM and 10 μM), 8-iso(15R)PGF (1 μM) and 8-isoPGE1 (1 μM). Although the EP1/EP2 antagonist, AH 6809 (10 μM) had no significant effect on K+-induced [ 3H]dopamine release, it blocked the inhibitory effect of both 8-isoPGE1 (10 μM) and 8-isoPGE2 (10 μM). In conclusion, IsoPs attenuate K+-induced [3H]dopamine release in isolated bovine retinae, presumably via an indirect action on COX pathway leading to the production of prostanoids, which in turn, activates EP receptors.

Original languageEnglish
Pages (from-to)37-42
Number of pages6
JournalNeurochemical Research
Volume33
Issue number1
DOIs
Publication statusPublished - Jan 2008

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All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry

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