Prestin and the dynamic stiffness of cochlear outer hair cells

David Z. He, Shuping Jia, Peter Dallos

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The outer hair cell (OHC) lateral wall is a unique trilaminate structure consisting of the plasma membrane, the cortical lattice, and subsurface cisternae. OHCs are capable of altering their length in response to transmembrane voltage change. This so-called electromotile response is presumed to result from conformational changes of membrane-bound protein molecules, named prestin. OHC motility is accompanied by axial stiffness changes when the membrane potential of the cell is altered. During length changes, intracellular anions (mainly Cl-) act as extrinsic voltage sensors. In this study, we inquired whether the motor proteins are responsible for the voltage-dependent axial stiffness of OHCs, and whether ACh, the neurotransmitter of efferent neurons, modulates the stiffness of the cortical lattice and/or the stiffness of the motor protein. The experiments were done on isolated guinea pig OHCs in the whole-cell voltage-clamp mode. Axial stiffness was determined by loading a fiber of known stiffness onto the apical surface of the cells. Voltage-dependent stiffness and cell motility disappeared, and the axial stiffness of the cells significantly decreased after removal of intracellular Cl-. The result suggests that the stiffness of the motor protein is a major contributor to the global axial stiffness of OHCs. ACh was found to affect both the motor protein and other lateral wall stiffness components.

Original languageEnglish
Pages (from-to)9089-9096
Number of pages8
JournalJournal of Neuroscience
Volume23
Issue number27
StatePublished - Oct 8 2003

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Outer Auditory Hair Cells
Cell Movement
Proteins
Efferent Neurons
Membrane Potentials
Cell Wall
Anions
Neurotransmitter Agents
Guinea Pigs
Membrane Proteins
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Prestin and the dynamic stiffness of cochlear outer hair cells. / He, David Z.; Jia, Shuping; Dallos, Peter.

In: Journal of Neuroscience, Vol. 23, No. 27, 08.10.2003, p. 9089-9096.

Research output: Contribution to journalArticle

He, DZ, Jia, S & Dallos, P 2003, 'Prestin and the dynamic stiffness of cochlear outer hair cells', Journal of Neuroscience, vol. 23, no. 27, pp. 9089-9096.
He, David Z. ; Jia, Shuping ; Dallos, Peter. / Prestin and the dynamic stiffness of cochlear outer hair cells. In: Journal of Neuroscience. 2003 ; Vol. 23, No. 27. pp. 9089-9096.
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