Primary structure and biological activity of a third gonadotropin-releasing hormone from lamprey brain

Stacia A. Sower, Yueh Chin Chiang, Sándor Lovas, J. Michael Conlon

Research output: Contribution to journalArticle

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Abstract

Previous studies have led to the identification of two molecular forms of GnRH (GnRH-I and II) in the brain of the sea lamprey, Petromyzon marinus. From analysis of these two forms, the primary structure of GnRH-I and the amino acid composition of GnRH-II were determined. We have now isolated a third molecular form of GnRH (GnRH-III) from the brain of this species that is different from GnRH-I and -II. The primary structure of GnRH-III is pGlu-His-Trp-Ser-His-Asp-Trp-Lys-Pro-Gly-NH2. A synthetic decapeptide with this amino acid sequence was chromatographically identical to natural GnRH-III. Intraperitoneal injection of synthetic lamprey GnRH-III (0.1 μg/g) produced a significant (P <0.05) elevation of plasma progesterone (31% over basal values) in female lampreys that was comparable to that produced by the same dose of lamprey GnRH-I (36% over basal). The elevation in plasma estradiol produced by lamprey GnRH-III (244% over basal) was significantly (P <0.05) less than the elevation produced by GnRH-I (322% over basal). We propose based on the biological activity of lamprey GnRH-III in these studies and the occurrence of this peptide during metamorphosis in lampreys, that both lamprey GnRH-I and -III are neurohormones involved in reproduction in lampreys.

Original languageEnglish
Pages (from-to)1125-1131
Number of pages7
JournalEndocrinology
Volume132
Issue number3
StatePublished - Mar 1993

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Lampreys
Gonadotropin-Releasing Hormone
Brain
Petromyzon
gonadotropin-releasing hormone-III
Intraperitoneal Injections
Reproduction
Progesterone
Neurotransmitter Agents
Amino Acid Sequence
Estradiol
Amino Acids
Peptides

All Science Journal Classification (ASJC) codes

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Primary structure and biological activity of a third gonadotropin-releasing hormone from lamprey brain. / Sower, Stacia A.; Chiang, Yueh Chin; Lovas, Sándor; Conlon, J. Michael.

In: Endocrinology, Vol. 132, No. 3, 03.1993, p. 1125-1131.

Research output: Contribution to journalArticle

Sower, Stacia A. ; Chiang, Yueh Chin ; Lovas, Sándor ; Conlon, J. Michael. / Primary structure and biological activity of a third gonadotropin-releasing hormone from lamprey brain. In: Endocrinology. 1993 ; Vol. 132, No. 3. pp. 1125-1131.
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