Primary structure and pharmacological activity of a nonapeptide related to neuromedin U isolated from chicken intestine

Finbarr O'Harte, Charles Bockman, Wanyun Zeng, Peter W. Abel, Steven Harvey, J. Michael Conlon

Research output: Contribution to journalArticle

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Abstract

An extract of chicken intestine contained neuromedin U-like immunoreactivity (36 pmol/g wet tissue weight). The primary structure of the predominant molecular form (NMU-9), comprising 94% of the total immunoreactivity, was established as: Gly-Tyr-Phe-Phe-Phe-Arg-Pro-Arg-Asn-NH2. This sequence differs from that of pig neuromedin U-8 (NMU-8) by the substitution of Leu3 by Phe and, like the corresponding peptide from the guinea pig, is extended from the NH2-terminus by a Gly residue. A minor component of neuromedin U comprised 25 amino acid residues. An extract of chicken whole brain contained much less NMU-like immunoreactivity (1.5 pmol/g) and the nonapeptide was the only molecular form detected. Synthetic chicken NMU-9 produced a concentration-dependent contraction of smooth muscle from the rat uterus and its effect was unchanged in the presence of tetrodotoxin, atropine and indomethacin. The potency of chicken NMU-9 (EC50 360±60 nM; mean±S.E., n=6) was approximately 8-fold less than that of pig NMU-8 (EC50 46±8 nM) but the maximum contraction produced by both agonists was not significantly different.

Original languageEnglish
Pages (from-to)809-812
Number of pages4
JournalPeptides
Volume12
Issue number4
DOIs
StatePublished - 1991

Fingerprint

neuromedin U 8
Intestines
Chickens
Pharmacology
Swine
Tetrodotoxin
Molecular Structure
Atropine
Indomethacin
Uterus
Smooth Muscle
Muscle
Rats
Brain
Guinea Pigs
Substitution reactions
Tissue
Amino Acids
Weights and Measures
Peptides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Primary structure and pharmacological activity of a nonapeptide related to neuromedin U isolated from chicken intestine. / O'Harte, Finbarr; Bockman, Charles; Zeng, Wanyun; Abel, Peter W.; Harvey, Steven; Conlon, J. Michael.

In: Peptides, Vol. 12, No. 4, 1991, p. 809-812.

Research output: Contribution to journalArticle

O'Harte, Finbarr ; Bockman, Charles ; Zeng, Wanyun ; Abel, Peter W. ; Harvey, Steven ; Conlon, J. Michael. / Primary structure and pharmacological activity of a nonapeptide related to neuromedin U isolated from chicken intestine. In: Peptides. 1991 ; Vol. 12, No. 4. pp. 809-812.
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