Prion infection of skeletal muscle cells and papillae in the tongue

Ellyn R. Mulcahy, Jason C. Bartz, Anthony Kincaid, Richard A. Bessen

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The presence of the prion agent in skeletal muscle is thought to be due to the infection of nerve fibers located within the muscle. We report here that the pathological isoform of the prion protein, PrPSc, accumulates within skeletal muscle cells, in addition to axons, in the tongue of hamsters following intralingual and intracerebral inoculation of the HY strain of the transmissible mink encephalopathy agent. Localization of PrPSc to the neuromuscular junction suggests that this synapse is a site for prion agent spread between motor axon terminals and muscle cells. Following intracerebral inoculation, the majority of PrPSc in the tongue was found in the lamina propria, where it was associated with sensory nerve fibers in the core of the lingual papillae. PrPSc staining was also identified in the stratified squamous epithelium of the lingual mucosa. These findings indicate that prion infection of skeletal muscle cells and the epithelial layer in the tongue can be established following the spread of the prion agent from nerve terminals and/or axons that innervate the tongue. Our data suggest that ingestion of meat products containing prion-infected tongue could result in human exposure to the prion agent, while sloughing of prion-infected epithelial cells at the mucosal surface of the tongue could be a mechanism for prion agent shedding and subsequent prion transmission in animals.

Original languageEnglish
Pages (from-to)6792-6798
Number of pages7
JournalJournal of Virology
Volume78
Issue number13
DOIs
StatePublished - Jul 2004

Fingerprint

Prions
prions
tongue
Tongue
myocytes
Muscle Cells
skeletal muscle
Skeletal Muscle
PrPSc proteins
Infection
infection
axons
Presynaptic Terminals
Nerve Fibers
nerve fibers
Mucous Membrane
transmissible mink encephalopathy
Mink
Meat Products
Neuromuscular Junction

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Prion infection of skeletal muscle cells and papillae in the tongue. / Mulcahy, Ellyn R.; Bartz, Jason C.; Kincaid, Anthony; Bessen, Richard A.

In: Journal of Virology, Vol. 78, No. 13, 07.2004, p. 6792-6798.

Research output: Contribution to journalArticle

Mulcahy, Ellyn R. ; Bartz, Jason C. ; Kincaid, Anthony ; Bessen, Richard A. / Prion infection of skeletal muscle cells and papillae in the tongue. In: Journal of Virology. 2004 ; Vol. 78, No. 13. pp. 6792-6798.
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