Processing of mouse proglucagon by recombinant prohormone convertase 1 and immunopurified prohormone convertase 2 in vitro

M. E. Rothenberg, C. D. Eilertson, K. Klein, Y. Zhou, I. Lindberg, J. K. McDonald, Robert Mackin, B. D. Noe

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The mouse tumor cell line αrTC1-6 was used as a model system to examine the post-translational processing of proglucagon. Determination of the mouse preproglucagon cDNA sequence and comparison with the published sequences of rat and human preproglucagons revealed nucleic acid homologies of 89.1 and 84%, respectively, and amino acid homologies of 94 and 89.4%, respectively. Immunohistochemical analyses with antibodies directed against PC2 and glucagon colocalized both the enzyme and substrate within the same secretory granules. PC1 was also immunolocalized in secretory granules. Cells were metabolically labeled with [3H]tryptophan, and extracts were analyzed by reverse-phase high pressure liquid chromatography. Radioactive peptides with retention times identical to those of synthetic peptide standards were recovered and subjected to peptide mapping to verify their identities. To determine the potential role of PC1 and PC2 in proglucagon processing, 3H- labeled proglucagon was incubated in vitro with recombinant PC1 and/or immunopurified PC2. Both enzymes cleaved proglucagon to yield the major proglucagon fragment, glicentin, and oxyntomodulin, whereas only PC1 released glucagon-like peptide-1 from the major proglucagon fragment. Neither PC1 nor PC2 processed glucagon from proglucagon in vitro. These results suggest a potential role for PC1 and/or PC2 in cleaving several of the normal products, excluding glucagon, from the mouse proglucagon precursor.

Original languageEnglish
Pages (from-to)10136-10146
Number of pages11
JournalJournal of Biological Chemistry
Volume270
Issue number17
DOIs
StatePublished - 1995
Externally publishedYes

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Proprotein Convertase 2
Proprotein Convertase 1
Proglucagon
Processing
Glucagon
Secretory Vesicles
Peptides
Glicentin
Oxyntomodulin
In Vitro Techniques
High pressure liquid chromatography
Glucagon-Like Peptide 1
Peptide Mapping
Reverse-Phase Chromatography
Enzymes
Tumor Cell Line
Tryptophan
Nucleic Acids
Rats
Tumors

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Rothenberg, M. E., Eilertson, C. D., Klein, K., Zhou, Y., Lindberg, I., McDonald, J. K., ... Noe, B. D. (1995). Processing of mouse proglucagon by recombinant prohormone convertase 1 and immunopurified prohormone convertase 2 in vitro. Journal of Biological Chemistry, 270(17), 10136-10146. https://doi.org/10.1074/jbc.270.17.10136

Processing of mouse proglucagon by recombinant prohormone convertase 1 and immunopurified prohormone convertase 2 in vitro. / Rothenberg, M. E.; Eilertson, C. D.; Klein, K.; Zhou, Y.; Lindberg, I.; McDonald, J. K.; Mackin, Robert; Noe, B. D.

In: Journal of Biological Chemistry, Vol. 270, No. 17, 1995, p. 10136-10146.

Research output: Contribution to journalArticle

Rothenberg, M. E. ; Eilertson, C. D. ; Klein, K. ; Zhou, Y. ; Lindberg, I. ; McDonald, J. K. ; Mackin, Robert ; Noe, B. D. / Processing of mouse proglucagon by recombinant prohormone convertase 1 and immunopurified prohormone convertase 2 in vitro. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 17. pp. 10136-10146.
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