Proteomic analysis of the organ of corti using nanoscale liquid chromatography coupled with tandem mass spectrometry

Hong Peng, Miao Liu, Jason Pecka, Kirk Beisel, Shi Jian Ding

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The organ of Corti (OC) in the cochlea plays an essential role in auditory signal transduction in the inner ear. For its minute size and trace amount of proteins, the identification of the molecules in pathophysiologic processes in the bone-encapsulated OC requires both delicate separation and a highly sensitive analytical tool. Previously, we reported the development of a high resolution metal-free nanoscale liquid chromatography system for highly sensitive phosphoproteomic analysis. Here this system was coupled with a LTQ-Orbitrap XL mass spectrometer to investigate the OC proteome from normal hearing FVB/N male mice. A total of 628 proteins were identified from six replicates of single LC-MS/MS analysis, with a false discovery rate of 1% using the decoy database approach by the OMSSA search engine. This is currently the largest proteome dataset for the OC. A total of 11 proteins, including cochlin, myosin VI, and myosin IX, were identified that when defective are associated with hearing impairment or loss. This study demonstrated the effectiveness of our nanoLC-MS/MS platform for sensitive identification of hearing loss-associated proteins from minute amount of tissue samples.

Original languageEnglish
Pages (from-to)8171-8188
Number of pages18
JournalInternational Journal of Molecular Sciences
Volume13
Issue number7
DOIs
StatePublished - 2012

Fingerprint

Organ of Corti
Liquid chromatography
liquid chromatography
Tandem Mass Spectrometry
Liquid Chromatography
organs
Proteomics
Mass spectrometry
Audition
mass spectroscopy
Hearing Loss
proteome
proteins
Proteins
myosins
Proteome
hearing
auditory signals
decoys
Search Engine

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Medicine(all)

Cite this

Proteomic analysis of the organ of corti using nanoscale liquid chromatography coupled with tandem mass spectrometry. / Peng, Hong; Liu, Miao; Pecka, Jason; Beisel, Kirk; Ding, Shi Jian.

In: International Journal of Molecular Sciences, Vol. 13, No. 7, 2012, p. 8171-8188.

Research output: Contribution to journalArticle

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