Proteomic fingerprinting of HIV-1-infected human monocyte-derived macrophages: A preliminary report

Kimberly A. Carlson, Pawel Ciborowski, Courtney N. Schellpeper, Toni M. Biskup, Rong Fong Shen, Xiaoguang Luo, Christopher J. Destache, Howard E. Gendelman

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Abstract

Mononuclear phagocytes (MP; blood monocytes, alveolar, lymph node, and brain macrophages and microglia) are vehicles for dissemination and principle target cells for human immunodeficiency virus type 1 (HIV-1) infection. Notably, viral persistence in macrophages occurs despite ongoing phagocytic, intracellular killing, innate and adaptive immune responses. To assess potential pathways for how HIV-1 may bypass antiviral MP responses, we used proteomic tests to evaluate protein fingerprints of HIV-1-infected human monocyte-derived macrophages 7 days after viral infection. By using weak cation exchange chips, 58 proteins were found up- or down-regulated after HIV-1 ADA infection. Several of these proteins were identified by microsequencing. It is probable that cellular proteins identified by proteomic fingerprinting could assist in unraveling how persistent viral infection occurs in MP lineage cells. Moreover, this evolving technology can be utilized to unravel changes in immune activities initiated by interactions between virus, environmental cues and drugs of abuse.

Original languageEnglish
Pages (from-to)35-42
Number of pages8
JournalJournal of Neuroimmunology
Volume147
Issue number1-2
DOIs
Publication statusPublished - Feb 2004

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All Science Journal Classification (ASJC) codes

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Carlson, K. A., Ciborowski, P., Schellpeper, C. N., Biskup, T. M., Shen, R. F., Luo, X., ... Gendelman, H. E. (2004). Proteomic fingerprinting of HIV-1-infected human monocyte-derived macrophages: A preliminary report. Journal of Neuroimmunology, 147(1-2), 35-42. https://doi.org/10.1016/j.jneuroim.2003.10.039