Proteomic fingerprints distinguish microglia, bone marrow, and spleen macrophage populations

Yoshimi Enose, Christopher J. Destache, Andrea L. Mack, James R. Anderson, Fred Ullrich, Pawel S. Ciborowski, Howard E. Gendelman

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Mononuclear phagocytes (MP; dendritic cells, monocytes, tissue macrophages, and microglia) maintain tissue homeostasis and provide a first line of defense against invading pathogens. In specific circumstances, MPs also induce inflammatory responses and as such affect disease onset and progression. Despite intensive research into MP biology, little is known of the functional and molecular properties of individual MP subtypes. Using a novel proteomics platform, unique protein patterns and protein identities were observed among populations of spleen and bone marrow macrophages and microglia. Cells were obtained from C57BL/6 mice and were cultivated in macrophage colony-stimulating factor. MP subtypes were indistinguishable by morphological or antigenic criteria. Protein profiling by Surface Enhanced Laser Desorption Ionization-Time of Flight (SELDI-TOF) ProteinChip® assays with weak cationic exchange chips showed unique MP spectral profiles. Corresponding protein fractions were recovered by high performance liquid chromatography and identified by liquid chromatography tandem mass spectrometry. The results provide a unique means to distinguish microglia from other MP subtypes.

Original languageEnglish
Pages (from-to)161-172
Number of pages12
JournalGLIA
Volume51
Issue number3
DOIs
StatePublished - Aug 15 2005

Fingerprint

Dermatoglyphics
Microglia
Proteomics
Spleen
Bone Marrow
Macrophages
Population
Protein Array Analysis
Proteins
Macrophage Colony-Stimulating Factor
Phagocytes
Tandem Mass Spectrometry
Inbred C57BL Mouse
Liquid Chromatography
Dendritic Cells
Disease Progression
Monocytes
Membrane Proteins
Lasers
Homeostasis

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Enose, Y., Destache, C. J., Mack, A. L., Anderson, J. R., Ullrich, F., Ciborowski, P. S., & Gendelman, H. E. (2005). Proteomic fingerprints distinguish microglia, bone marrow, and spleen macrophage populations. GLIA, 51(3), 161-172. https://doi.org/10.1002/glia.20193

Proteomic fingerprints distinguish microglia, bone marrow, and spleen macrophage populations. / Enose, Yoshimi; Destache, Christopher J.; Mack, Andrea L.; Anderson, James R.; Ullrich, Fred; Ciborowski, Pawel S.; Gendelman, Howard E.

In: GLIA, Vol. 51, No. 3, 15.08.2005, p. 161-172.

Research output: Contribution to journalArticle

Enose, Y, Destache, CJ, Mack, AL, Anderson, JR, Ullrich, F, Ciborowski, PS & Gendelman, HE 2005, 'Proteomic fingerprints distinguish microglia, bone marrow, and spleen macrophage populations', GLIA, vol. 51, no. 3, pp. 161-172. https://doi.org/10.1002/glia.20193
Enose, Yoshimi ; Destache, Christopher J. ; Mack, Andrea L. ; Anderson, James R. ; Ullrich, Fred ; Ciborowski, Pawel S. ; Gendelman, Howard E. / Proteomic fingerprints distinguish microglia, bone marrow, and spleen macrophage populations. In: GLIA. 2005 ; Vol. 51, No. 3. pp. 161-172.
@article{83fa652f244a4ad1848649bfa345770b,
title = "Proteomic fingerprints distinguish microglia, bone marrow, and spleen macrophage populations",
abstract = "Mononuclear phagocytes (MP; dendritic cells, monocytes, tissue macrophages, and microglia) maintain tissue homeostasis and provide a first line of defense against invading pathogens. In specific circumstances, MPs also induce inflammatory responses and as such affect disease onset and progression. Despite intensive research into MP biology, little is known of the functional and molecular properties of individual MP subtypes. Using a novel proteomics platform, unique protein patterns and protein identities were observed among populations of spleen and bone marrow macrophages and microglia. Cells were obtained from C57BL/6 mice and were cultivated in macrophage colony-stimulating factor. MP subtypes were indistinguishable by morphological or antigenic criteria. Protein profiling by Surface Enhanced Laser Desorption Ionization-Time of Flight (SELDI-TOF) ProteinChip{\circledR} assays with weak cationic exchange chips showed unique MP spectral profiles. Corresponding protein fractions were recovered by high performance liquid chromatography and identified by liquid chromatography tandem mass spectrometry. The results provide a unique means to distinguish microglia from other MP subtypes.",
author = "Yoshimi Enose and Destache, {Christopher J.} and Mack, {Andrea L.} and Anderson, {James R.} and Fred Ullrich and Ciborowski, {Pawel S.} and Gendelman, {Howard E.}",
year = "2005",
month = "8",
day = "15",
doi = "10.1002/glia.20193",
language = "English",
volume = "51",
pages = "161--172",
journal = "GLIA",
issn = "0894-1491",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

TY - JOUR

T1 - Proteomic fingerprints distinguish microglia, bone marrow, and spleen macrophage populations

AU - Enose, Yoshimi

AU - Destache, Christopher J.

AU - Mack, Andrea L.

AU - Anderson, James R.

AU - Ullrich, Fred

AU - Ciborowski, Pawel S.

AU - Gendelman, Howard E.

PY - 2005/8/15

Y1 - 2005/8/15

N2 - Mononuclear phagocytes (MP; dendritic cells, monocytes, tissue macrophages, and microglia) maintain tissue homeostasis and provide a first line of defense against invading pathogens. In specific circumstances, MPs also induce inflammatory responses and as such affect disease onset and progression. Despite intensive research into MP biology, little is known of the functional and molecular properties of individual MP subtypes. Using a novel proteomics platform, unique protein patterns and protein identities were observed among populations of spleen and bone marrow macrophages and microglia. Cells were obtained from C57BL/6 mice and were cultivated in macrophage colony-stimulating factor. MP subtypes were indistinguishable by morphological or antigenic criteria. Protein profiling by Surface Enhanced Laser Desorption Ionization-Time of Flight (SELDI-TOF) ProteinChip® assays with weak cationic exchange chips showed unique MP spectral profiles. Corresponding protein fractions were recovered by high performance liquid chromatography and identified by liquid chromatography tandem mass spectrometry. The results provide a unique means to distinguish microglia from other MP subtypes.

AB - Mononuclear phagocytes (MP; dendritic cells, monocytes, tissue macrophages, and microglia) maintain tissue homeostasis and provide a first line of defense against invading pathogens. In specific circumstances, MPs also induce inflammatory responses and as such affect disease onset and progression. Despite intensive research into MP biology, little is known of the functional and molecular properties of individual MP subtypes. Using a novel proteomics platform, unique protein patterns and protein identities were observed among populations of spleen and bone marrow macrophages and microglia. Cells were obtained from C57BL/6 mice and were cultivated in macrophage colony-stimulating factor. MP subtypes were indistinguishable by morphological or antigenic criteria. Protein profiling by Surface Enhanced Laser Desorption Ionization-Time of Flight (SELDI-TOF) ProteinChip® assays with weak cationic exchange chips showed unique MP spectral profiles. Corresponding protein fractions were recovered by high performance liquid chromatography and identified by liquid chromatography tandem mass spectrometry. The results provide a unique means to distinguish microglia from other MP subtypes.

UR - http://www.scopus.com/inward/record.url?scp=24144454254&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24144454254&partnerID=8YFLogxK

U2 - 10.1002/glia.20193

DO - 10.1002/glia.20193

M3 - Article

VL - 51

SP - 161

EP - 172

JO - GLIA

JF - GLIA

SN - 0894-1491

IS - 3

ER -