Pseudin-2

An antimicrobial peptide with low hemolytic activity from the skin of the paradoxical frog

Loyd Olson, Ana Maria Soto, Floyd C. Knoop, J. Michael Conlon

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Four structurally related peptides (pseudins 1-4) with antimicrobial activity were isolated from an extract of the skin of the paradoxical frog Pseudis paradoxa (Pseudidae). Pseudin-2 (GLNALKKVFQGIHEAIKLINNHVQ) was the most abundant peptide (22 nmol/g tissue) and also the most potent (minimum inhibitory concentrations, MIC = 2.5 μM against Escherichia coli, 80 μM against Staphylococcus aureus, and 130 μM against Candida albicans). The concentration of pseudin-2 producing 50% hemolysis of human erythrocytes was >300 μM. Circular dichroism studies showed that the pseudins belong to the class of cationic, amphipathic α-helical antimicrobial peptides but their amino acid sequences are not similar to any previously characterized peptides from frog skin. The pseudins do, however, show sequence similarity with a region at the C-terminus of DEFT, a death effector domain-containing protein expressed in mammalian testicular germ cells that is involved in the regulation of apoptosis.

Original languageEnglish (US)
Pages (from-to)1001-1005
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume288
Issue number4
DOIs
StatePublished - Nov 9 2001
Externally publishedYes

Fingerprint

Anura
Skin
Peptides
Death Domain Receptor Signaling Adaptor Proteins
Antimicrobial Cationic Peptides
Microbial Sensitivity Tests
Circular Dichroism
Hemolysis
Candida albicans
Germ Cells
Staphylococcus aureus
Candida
Amino Acid Sequence
Erythrocytes
Escherichia coli
Apoptosis
Cells
Tissue
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Pseudin-2 : An antimicrobial peptide with low hemolytic activity from the skin of the paradoxical frog. / Olson, Loyd; Soto, Ana Maria; Knoop, Floyd C.; Conlon, J. Michael.

In: Biochemical and Biophysical Research Communications, Vol. 288, No. 4, 09.11.2001, p. 1001-1005.

Research output: Contribution to journalArticle

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