Abstract
Class B β-lactamases are known as metallo-β-lactamases (MBLs) and they hydrolyze most β-lactams, including carbapenems. IMP-18, an MBL cloned from Pseudomonas aeruginosa, was overexpressed, purified and crystallized by vapour diffusion for X-ray crystallographic analysis. Preliminary X-ray analysis showed that the crystal diffracted to 2.4Å resolution and belonged to the tetragonal space group P41212, with unit-cell parameters a = b = 120.77, c = 96.54Å, = β = γ = 90°, suggesting the presence of two molecules in the asymmetric unit.
| Original language | English |
|---|---|
| Pages (from-to) | 1397-1400 |
| Number of pages | 4 |
| Journal | Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
| Volume | 69 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 2013 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Biophysics
- Structural Biology
- Genetics
- Condensed Matter Physics
Cite this
Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa. / Furuyama, Takamitsu; Ishii, Yoshikazu; Ohya, Norimasa; Tateda, Kazuhiro; Hanson, Nancy D.; Shimizu-Ibuka, Akiko.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 69, No. 12, 12.2013, p. 1397-1400.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa
AU - Furuyama, Takamitsu
AU - Ishii, Yoshikazu
AU - Ohya, Norimasa
AU - Tateda, Kazuhiro
AU - Hanson, Nancy D.
AU - Shimizu-Ibuka, Akiko
PY - 2013/12
Y1 - 2013/12
N2 - Class B β-lactamases are known as metallo-β-lactamases (MBLs) and they hydrolyze most β-lactams, including carbapenems. IMP-18, an MBL cloned from Pseudomonas aeruginosa, was overexpressed, purified and crystallized by vapour diffusion for X-ray crystallographic analysis. Preliminary X-ray analysis showed that the crystal diffracted to 2.4Å resolution and belonged to the tetragonal space group P41212, with unit-cell parameters a = b = 120.77, c = 96.54Å, = β = γ = 90°, suggesting the presence of two molecules in the asymmetric unit.
AB - Class B β-lactamases are known as metallo-β-lactamases (MBLs) and they hydrolyze most β-lactams, including carbapenems. IMP-18, an MBL cloned from Pseudomonas aeruginosa, was overexpressed, purified and crystallized by vapour diffusion for X-ray crystallographic analysis. Preliminary X-ray analysis showed that the crystal diffracted to 2.4Å resolution and belonged to the tetragonal space group P41212, with unit-cell parameters a = b = 120.77, c = 96.54Å, = β = γ = 90°, suggesting the presence of two molecules in the asymmetric unit.
UR - http://www.scopus.com/inward/record.url?scp=84890079769&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84890079769&partnerID=8YFLogxK
U2 - 10.1107/S1744309113030480
DO - 10.1107/S1744309113030480
M3 - Article
C2 - 24316839
AN - SCOPUS:84890079769
VL - 69
SP - 1397
EP - 1400
JO - Acta Crystallographica Section F:Structural Biology Communications
JF - Acta Crystallographica Section F:Structural Biology Communications
SN - 1744-3091
IS - 12
ER -