Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa

Takamitsu Furuyama; Yoshikazu Ishii; Norimasa Ohya; Kazuhiro Tateda; Nancy D. Hanson; Akiko Shimizu-Ibuka

doi:10.1107/S1744309113030480

Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa

Takamitsu Furuyama, Yoshikazu Ishii, Norimasa Ohya, Kazuhiro Tateda, Nancy D. Hanson, Akiko Shimizu-Ibuka

Department of Medical Microbiology and Immunology

Research output: Contribution to journal › Article

1 Scopus citations

Abstract

Class B β-lactamases are known as metallo-β-lactamases (MBLs) and they hydrolyze most β-lactams, including carbapenems. IMP-18, an MBL cloned from Pseudomonas aeruginosa, was overexpressed, purified and crystallized by vapour diffusion for X-ray crystallographic analysis. Preliminary X-ray analysis showed that the crystal diffracted to 2.4Å resolution and belonged to the tetragonal space group P41212, with unit-cell parameters a = b = 120.77, c = 96.54Å, = β = γ = 90°, suggesting the presence of two molecules in the asymmetric unit.

Original language	English (US)
Pages (from-to)	1397-1400
Number of pages	4
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	69
Issue number	12
DOIs	https://doi.org/10.1107/S1744309113030480
State	Published - Dec 1 2013

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All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

Cite this

Furuyama, T., Ishii, Y., Ohya, N., Tateda, K., Hanson, N. D., & Shimizu-Ibuka, A. (2013). Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 69(12), 1397-1400. https://doi.org/10.1107/S1744309113030480

Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa. / Furuyama, Takamitsu; Ishii, Yoshikazu; Ohya, Norimasa; Tateda, Kazuhiro; Hanson, Nancy D.; Shimizu-Ibuka, Akiko.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 69, No. 12, 01.12.2013, p. 1397-1400.

Research output: Contribution to journal › Article

Furuyama, Takamitsu ; Ishii, Yoshikazu ; Ohya, Norimasa ; Tateda, Kazuhiro ; Hanson, Nancy D. ; Shimizu-Ibuka, Akiko. / Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2013 ; Vol. 69, No. 12. pp. 1397-1400.

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10.1107/S1744309113030480