Abstract
Class B β-lactamases are known as metallo-β-lactamases (MBLs) and they hydrolyze most β-lactams, including carbapenems. IMP-18, an MBL cloned from Pseudomonas aeruginosa, was overexpressed, purified and crystallized by vapour diffusion for X-ray crystallographic analysis. Preliminary X-ray analysis showed that the crystal diffracted to 2.4Å resolution and belonged to the tetragonal space group P41212, with unit-cell parameters a = b = 120.77, c = 96.54Å, = β = γ = 90°, suggesting the presence of two molecules in the asymmetric unit.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1397-1400 |
| Number of pages | 4 |
| Journal | Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
| Volume | 69 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 1 2013 |
All Science Journal Classification (ASJC) codes
- Biophysics
- Structural Biology
- Biochemistry
- Genetics
- Condensed Matter Physics
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Furuyama, T., Ishii, Y., Ohya, N., Tateda, K., Hanson, N. D., & Shimizu-Ibuka, A. (2013). Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 69(12), 1397-1400. https://doi.org/10.1107/S1744309113030480