Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa

Takamitsu Furuyama, Yoshikazu Ishii, Norimasa Ohya, Kazuhiro Tateda, Nancy D. Hanson, Akiko Shimizu-Ibuka

Research output: Contribution to journalArticle

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Abstract

Class B β-lactamases are known as metallo-β-lactamases (MBLs) and they hydrolyze most β-lactams, including carbapenems. IMP-18, an MBL cloned from Pseudomonas aeruginosa, was overexpressed, purified and crystallized by vapour diffusion for X-ray crystallographic analysis. Preliminary X-ray analysis showed that the crystal diffracted to 2.4Å resolution and belonged to the tetragonal space group P41212, with unit-cell parameters a = b = 120.77, c = 96.54Å, = β = γ = 90°, suggesting the presence of two molecules in the asymmetric unit.

Original languageEnglish (US)
Pages (from-to)1397-1400
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume69
Issue number12
DOIs
StatePublished - Dec 1 2013

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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