Abstract
The enzymes responsible for performing cleavage of propeptides at basic amino acids have proven difficult to characterize. Using the processing of anglerfish islet prosomatostatin (PSS) as a model system, we are pursuing the characterization of both a single basic amino acid-specific and a dibasic amino acid-specific converting enzyme. We describe here the model system and protein isolation methods that have allowed significant progress toward complete characterization of the somatostatin-generating propeptide converting enzymes (PCEs).
| Original language | English |
|---|---|
| Pages (from-to) | 30-32 |
| Number of pages | 3 |
| Journal | Metabolism: Clinical and Experimental |
| Volume | 39 |
| Issue number | 9 SUPPL. 2 |
| DOIs | |
| State | Published - 1990 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Endocrinology
- Endocrinology, Diabetes and Metabolism
Cite this
Purification of prosomatostatin-converting enzymes. / Mackin, Robert; Noe, Bryan D.; Spiess, Joachim.
In: Metabolism: Clinical and Experimental, Vol. 39, No. 9 SUPPL. 2, 1990, p. 30-32.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Purification of prosomatostatin-converting enzymes
AU - Mackin, Robert
AU - Noe, Bryan D.
AU - Spiess, Joachim
PY - 1990
Y1 - 1990
N2 - The enzymes responsible for performing cleavage of propeptides at basic amino acids have proven difficult to characterize. Using the processing of anglerfish islet prosomatostatin (PSS) as a model system, we are pursuing the characterization of both a single basic amino acid-specific and a dibasic amino acid-specific converting enzyme. We describe here the model system and protein isolation methods that have allowed significant progress toward complete characterization of the somatostatin-generating propeptide converting enzymes (PCEs).
AB - The enzymes responsible for performing cleavage of propeptides at basic amino acids have proven difficult to characterize. Using the processing of anglerfish islet prosomatostatin (PSS) as a model system, we are pursuing the characterization of both a single basic amino acid-specific and a dibasic amino acid-specific converting enzyme. We describe here the model system and protein isolation methods that have allowed significant progress toward complete characterization of the somatostatin-generating propeptide converting enzymes (PCEs).
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U2 - 10.1016/0026-0495(90)90204-P
DO - 10.1016/0026-0495(90)90204-P
M3 - Article
VL - 39
SP - 30
EP - 32
JO - Metabolism: Clinical and Experimental
JF - Metabolism: Clinical and Experimental
SN - 0026-0495
IS - 9 SUPPL. 2
ER -