Quantifying cellular differentiation by physical phenotype using digital holographic microscopy.

Kevin J. Chalut, Andrew Ekpenyong, Warren L. Clegg, Isabel C. Melhuish, Jochen Guck

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Although the biochemical changes that occur during cell differentiation are well-known, less known is that there are significant, cell-wide physical changes that also occur. Understanding and quantifying these changes can help to better understand the process of differentiation as well as ways to monitor it. Digital holographic microscopy (DHM) is a marker-free quantitative phase microscopy technique for measuring biological processes such as cellular differentiation, alleviating the need for introduction of foreign markers. We found significant changes in subcellular structure and refractive index of differentiating myeloid precursor cells within one day of differentiation induction, and significant differences depending on the type of lineage commitment. We augmented our results by showing significant changes in the softness of myeloid precursor cell differentiation within one day using optical stretching, a laser trap-based marker-free technique. DHM and optical stretching therefore provide consequential parameterization of cellular differentiation with sensitivity otherwise difficult to achieve. Therefore, we provide a way forward to quantify and understand cell differentiation with minimal perturbation using biophotonics. This journal is

Original languageEnglish
Pages (from-to)280-284
Number of pages5
JournalIntegrative Biology
Volume4
Issue number3
DOIs
StatePublished - Mar 2012

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Cell Differentiation
Microscopy
Microscopic examination
Myeloid Cells
Phenotype
Stretching
Biological Phenomena
Refractometry
Parameterization
Refractive index
Lasers

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Quantifying cellular differentiation by physical phenotype using digital holographic microscopy. / Chalut, Kevin J.; Ekpenyong, Andrew; Clegg, Warren L.; Melhuish, Isabel C.; Guck, Jochen.

In: Integrative Biology, Vol. 4, No. 3, 03.2012, p. 280-284.

Research output: Contribution to journalArticle

Chalut, Kevin J. ; Ekpenyong, Andrew ; Clegg, Warren L. ; Melhuish, Isabel C. ; Guck, Jochen. / Quantifying cellular differentiation by physical phenotype using digital holographic microscopy. In: Integrative Biology. 2012 ; Vol. 4, No. 3. pp. 280-284.
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