Quantitation of leuprolide acetate by high performance liquid chromatography

J. Singh, S. K. Rastogi, S. N. Singh, J. S. Bhatia

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

An isocratic technique was developed for the analysis of leuprolide acetate by high performance liquid chromatography (HPLC) using 220 nm UV detection, C18 MICROSORB-MV™ column (4.6 mm×15 cm), mobile phase (0.03 M, 77% dibasic ammonium phosphate buffer: 23% acetonitrile), and 2.0 mL/min flow rate. The coefficient of variation (C.V.) for precision and proportionality assays was lower than 2% for all concentrations studied. The detection limit and tailing factor for leuprolide acetate were 100 ng/mL (signal-to-noise ratio of 3:1) and 1.16-1.26, respectively.

Original languageEnglish (US)
Pages (from-to)3023-3031
Number of pages9
JournalJournal of Liquid Chromatography and Related Technologies
Volume23
Issue number19
DOIs
StatePublished - Dec 1 2000

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

Cite this