Quantitation of leuprolide acetate by high performance liquid chromatography

J. Singh; S. K. Rastogi; S. N. Singh; J. S. Bhatia

doi:10.1081/JLC-100101840

Quantitation of leuprolide acetate by high performance liquid chromatography

J. Singh, S. K. Rastogi, S. N. Singh, J. S. Bhatia

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

An isocratic technique was developed for the analysis of leuprolide acetate by high performance liquid chromatography (HPLC) using 220 nm UV detection, C₁₈ MICROSORB-MV™ column (4.6 mm×15 cm), mobile phase (0.03 M, 77% dibasic ammonium phosphate buffer: 23% acetonitrile), and 2.0 mL/min flow rate. The coefficient of variation (C.V.) for precision and proportionality assays was lower than 2% for all concentrations studied. The detection limit and tailing factor for leuprolide acetate were 100 ng/mL (signal-to-noise ratio of 3:1) and 1.16-1.26, respectively.

Original language	English (US)
Pages (from-to)	3023-3031
Number of pages	9
Journal	Journal of Liquid Chromatography and Related Technologies
Volume	23
Issue number	19
DOIs	https://doi.org/10.1081/JLC-100101840
State	Published - Dec 1 2000
Externally published	Yes

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Pharmaceutical Science
Clinical Biochemistry

Access to Document

10.1081/JLC-100101840

Fingerprint Dive into the research topics of 'Quantitation of leuprolide acetate by high performance liquid chromatography'. Together they form a unique fingerprint.

Cite this

@article{98942cc1a690450598d3927565426f34,

title = "Quantitation of leuprolide acetate by high performance liquid chromatography",

abstract = "An isocratic technique was developed for the analysis of leuprolide acetate by high performance liquid chromatography (HPLC) using 220 nm UV detection, C18 MICROSORB-MV{\texttrademark} column (4.6 mm×15 cm), mobile phase (0.03 M, 77% dibasic ammonium phosphate buffer: 23% acetonitrile), and 2.0 mL/min flow rate. The coefficient of variation (C.V.) for precision and proportionality assays was lower than 2% for all concentrations studied. The detection limit and tailing factor for leuprolide acetate were 100 ng/mL (signal-to-noise ratio of 3:1) and 1.16-1.26, respectively.",

author = "J. Singh and Rastogi, {S. K.} and Singh, {S. N.} and Bhatia, {J. S.}",

year = "2000",

month = dec,

day = "1",

doi = "10.1081/JLC-100101840",

language = "English (US)",

volume = "23",

pages = "3023--3031",

journal = "Journal of Liquid Chromatography and Related Technologies",

issn = "1082-6076",

publisher = "Taylor and Francis Ltd.",

number = "19",

}

TY - JOUR

T1 - Quantitation of leuprolide acetate by high performance liquid chromatography

AU - Singh, J.

AU - Rastogi, S. K.

AU - Singh, S. N.

AU - Bhatia, J. S.

PY - 2000/12/1

Y1 - 2000/12/1

N2 - An isocratic technique was developed for the analysis of leuprolide acetate by high performance liquid chromatography (HPLC) using 220 nm UV detection, C18 MICROSORB-MV™ column (4.6 mm×15 cm), mobile phase (0.03 M, 77% dibasic ammonium phosphate buffer: 23% acetonitrile), and 2.0 mL/min flow rate. The coefficient of variation (C.V.) for precision and proportionality assays was lower than 2% for all concentrations studied. The detection limit and tailing factor for leuprolide acetate were 100 ng/mL (signal-to-noise ratio of 3:1) and 1.16-1.26, respectively.

AB - An isocratic technique was developed for the analysis of leuprolide acetate by high performance liquid chromatography (HPLC) using 220 nm UV detection, C18 MICROSORB-MV™ column (4.6 mm×15 cm), mobile phase (0.03 M, 77% dibasic ammonium phosphate buffer: 23% acetonitrile), and 2.0 mL/min flow rate. The coefficient of variation (C.V.) for precision and proportionality assays was lower than 2% for all concentrations studied. The detection limit and tailing factor for leuprolide acetate were 100 ng/mL (signal-to-noise ratio of 3:1) and 1.16-1.26, respectively.

UR - http://www.scopus.com/inward/record.url?scp=0034424890&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034424890&partnerID=8YFLogxK

U2 - 10.1081/JLC-100101840

DO - 10.1081/JLC-100101840

M3 - Article

AN - SCOPUS:0034424890

VL - 23

SP - 3023

EP - 3031

JO - Journal of Liquid Chromatography and Related Technologies

JF - Journal of Liquid Chromatography and Related Technologies

SN - 1082-6076

IS - 19

ER -

Quantitation of leuprolide acetate by high performance liquid chromatography

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Cite this