Abstract
An isocratic technique was developed for the analysis of leuprolide acetate by high performance liquid chromatography (HPLC) using 220 nm UV detection, C18 MICROSORB-MV™ column (4.6 mm×15 cm), mobile phase (0.03 M, 77% dibasic ammonium phosphate buffer: 23% acetonitrile), and 2.0 mL/min flow rate. The coefficient of variation (C.V.) for precision and proportionality assays was lower than 2% for all concentrations studied. The detection limit and tailing factor for leuprolide acetate were 100 ng/mL (signal-to-noise ratio of 3:1) and 1.16-1.26, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 3023-3031 |
| Number of pages | 9 |
| Journal | Journal of Liquid Chromatography and Related Technologies |
| Volume | 23 |
| Issue number | 19 |
| DOIs | |
| State | Published - 2000 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Clinical Biochemistry
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Quantitation of leuprolide acetate by high performance liquid chromatography. / Singh, J.; Rastogi, S. K.; Singh, Somnath; Bhatia, J. S.
In: Journal of Liquid Chromatography and Related Technologies, Vol. 23, No. 19, 2000, p. 3023-3031.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitation of leuprolide acetate by high performance liquid chromatography
AU - Singh, J.
AU - Rastogi, S. K.
AU - Singh, Somnath
AU - Bhatia, J. S.
PY - 2000
Y1 - 2000
N2 - An isocratic technique was developed for the analysis of leuprolide acetate by high performance liquid chromatography (HPLC) using 220 nm UV detection, C18 MICROSORB-MV™ column (4.6 mm×15 cm), mobile phase (0.03 M, 77% dibasic ammonium phosphate buffer: 23% acetonitrile), and 2.0 mL/min flow rate. The coefficient of variation (C.V.) for precision and proportionality assays was lower than 2% for all concentrations studied. The detection limit and tailing factor for leuprolide acetate were 100 ng/mL (signal-to-noise ratio of 3:1) and 1.16-1.26, respectively.
AB - An isocratic technique was developed for the analysis of leuprolide acetate by high performance liquid chromatography (HPLC) using 220 nm UV detection, C18 MICROSORB-MV™ column (4.6 mm×15 cm), mobile phase (0.03 M, 77% dibasic ammonium phosphate buffer: 23% acetonitrile), and 2.0 mL/min flow rate. The coefficient of variation (C.V.) for precision and proportionality assays was lower than 2% for all concentrations studied. The detection limit and tailing factor for leuprolide acetate were 100 ng/mL (signal-to-noise ratio of 3:1) and 1.16-1.26, respectively.
UR - http://www.scopus.com/inward/record.url?scp=0034424890&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034424890&partnerID=8YFLogxK
U2 - 10.1081/JLC-100101840
DO - 10.1081/JLC-100101840
M3 - Article
VL - 23
SP - 3023
EP - 3031
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
SN - 1082-6076
IS - 19
ER -