Abstract
An isocratic technique was developed for the analysis of leuprolide acetate by high performance liquid chromatography (HPLC) using 220 nm UV detection, C18 MICROSORB-MV™ column (4.6 mm×15 cm), mobile phase (0.03 M, 77% dibasic ammonium phosphate buffer: 23% acetonitrile), and 2.0 mL/min flow rate. The coefficient of variation (C.V.) for precision and proportionality assays was lower than 2% for all concentrations studied. The detection limit and tailing factor for leuprolide acetate were 100 ng/mL (signal-to-noise ratio of 3:1) and 1.16-1.26, respectively.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3023-3031 |
| Number of pages | 9 |
| Journal | Journal of Liquid Chromatography and Related Technologies |
| Volume | 23 |
| Issue number | 19 |
| DOIs | |
| State | Published - Dec 1 2000 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Pharmaceutical Science
- Clinical Biochemistry
Cite this
Singh, J., Rastogi, S. K., Singh, S. N., & Bhatia, J. S. (2000). Quantitation of leuprolide acetate by high performance liquid chromatography. Journal of Liquid Chromatography and Related Technologies, 23(19), 3023-3031. https://doi.org/10.1081/JLC-100101840