Quantitation of leuprolide acetate by high performance liquid chromatography

J. Singh; S. K. Rastogi; S. N. Singh; J. S. Bhatia

doi:10.1081/JLC-100101840

Quantitation of leuprolide acetate by high performance liquid chromatography

J. Singh, S. K. Rastogi, S. N. Singh, J. S. Bhatia

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8 Scopus citations

Abstract

An isocratic technique was developed for the analysis of leuprolide acetate by high performance liquid chromatography (HPLC) using 220 nm UV detection, C₁₈ MICROSORB-MV™ column (4.6 mm×15 cm), mobile phase (0.03 M, 77% dibasic ammonium phosphate buffer: 23% acetonitrile), and 2.0 mL/min flow rate. The coefficient of variation (C.V.) for precision and proportionality assays was lower than 2% for all concentrations studied. The detection limit and tailing factor for leuprolide acetate were 100 ng/mL (signal-to-noise ratio of 3:1) and 1.16-1.26, respectively.

Original language	English (US)
Pages (from-to)	3023-3031
Number of pages	9
Journal	Journal of Liquid Chromatography and Related Technologies
Volume	23
Issue number	19
DOIs	https://doi.org/10.1081/JLC-100101840
State	Published - Dec 1 2000
Externally published	Yes

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Pharmaceutical Science
Clinical Biochemistry

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10.1081/JLC-100101840

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AU - Rastogi, S. K.

AU - Singh, S. N.

AU - Bhatia, J. S.

PY - 2000/12/1

Y1 - 2000/12/1

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AB - An isocratic technique was developed for the analysis of leuprolide acetate by high performance liquid chromatography (HPLC) using 220 nm UV detection, C18 MICROSORB-MV™ column (4.6 mm×15 cm), mobile phase (0.03 M, 77% dibasic ammonium phosphate buffer: 23% acetonitrile), and 2.0 mL/min flow rate. The coefficient of variation (C.V.) for precision and proportionality assays was lower than 2% for all concentrations studied. The detection limit and tailing factor for leuprolide acetate were 100 ng/mL (signal-to-noise ratio of 3:1) and 1.16-1.26, respectively.

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Quantitation of leuprolide acetate by high performance liquid chromatography

Abstract

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